Che, Shiyi Feng, Xue Li, Zhengjun Su, Zhiguo Ma, Guanghui Li, Zhikao Yu, Aibing Liu, Minsu Zhang, Songping
Published in
Biotechnology and bioengineering
The messenger RNA (mRNA) 5'-cap structure is indispensable for mRNA translation initiation and stability. Despite its importance, large-scale production of capped mRNA through in vitro transcription (IVT) synthesis using vaccinia capping enzyme (VCE) is challenging, due to the requirement of tedious and multiple pre-and-post separation steps causin...
Ogando, Natacha S. El Kazzi, Priscila Zevenhoven-Dobbe, Jessika C. Bontes, Brenda W. Decombe, Alice Posthuma, Clara C. Thiel, Volker Canard, Bruno Ferron, François Decroly, Etienne
...
Published in
Proceedings of the National Academy of Sciences of the United States of America
As coronaviruses (CoVs) replicate in the host cell cytoplasm, they rely on their own capping machinery to ensure the efficient translation of their messenger RNAs (mRNAs), protect them from degradation by cellular 5′ exoribonucleases (ExoNs), and escape innate immune sensing. The CoV nonstructural protein 14 (nsp14) is a bifunctional replicase subu...
Yan, Liming Yang, Yunxiang Li, Mingyu Zhang, Ying Zheng, Litao Ge, Ji Huang, Yucen C Liu, Zhenyu Wang, Tao Gao, Shan
...
Published in
Cell
The capping of mRNA and the proofreading play essential roles in SARS-CoV-2 replication and transcription. Here, we present the cryo-EM structure of the SARS-CoV-2 replication-transcription complex (RTC) in a form identified as Cap(0)-RTC, which couples a co-transcriptional capping complex (CCC) composed of nsp12 NiRAN, nsp9, the bifunctional nsp14...
Du, Xuejian Gao, Zeng-Qiang Geng, Zhi Dong, Yu-Hui Zhang, Heng
Published in
Journal of virology
African swine fever virus (ASFV) is a complex nucleocytoplasmic large DNA virus (NCLDV) that causes a devastating swine disease and it is urgently needed to develop effective anti-ASFV vaccines and drugs. The process of mRNA 5'-end capping is a common characteristic in eukaryotes and many viruses, and the cap structure is required for mRNA stabilit...
Alshiraihi, Ilham M. Klein, Gerald L. Brown, Mark A.
Published in
Diseases
With the approval and distribution of demonstrably safe COVID-19 vaccines bearing exceptionally high efficacy profiles, it may be tempting to envision a return to “normal” in the coming months. However, if there is one lesson to be learned from the ongoing pandemic, it is that, in a world of evolving zoonotic viruses, we must be better prepared for...
Bell, Philip J L
Published in
Virus research
The defining feature of the eukaryotic cell is the possession of a nucleus that uncouples transcription from translation. According to the updated Viral Eukaryogenesis (VE) hypothesis presented here, the eukaryotic nucleus descends from the viral factory of a DNA virus that infected the archaeal ancestor of the eukaryotes. The VE hypothesis implies...
Aoi, Yuki Smith, Edwin R Shah, Avani P Rendleman, Emily J Marshall, Stacy A Woodfin, Ashley R Chen, Fei X Shiekhattar, Ramin Shilatifard, Ali
Published in
Molecular cell
RNA polymerase II (RNA Pol II) is generally paused at promoter-proximal regions in most metazoans, and based on in vitro studies, this function has been attributed to the negative elongation factor (NELF). Here, we show that upon rapid depletion of NELF, RNA Pol II fails to be released into gene bodies, stopping instead around the +1 nucleosomal dy...
Kachaev, Zaur M. Lebedeva, Lyubov A. Kozlov, Eugene N. Shidlovskii, Yulii V.
Published in
Bioscience Reports
Early stages of transcription from eukaryotic promoters include two principal events: the capping of newly synthesized mRNA and the transition of RNA polymerase II from the preinitiation complex to the productive elongation state. The capping checkpoint model implies that these events are tightly coupled, which is necessary for ensuring the proper ...
Ogino, Tomoaki Green, Todd J.
Published in
Frontiers in Microbiology
Non-segmented negative strand (NNS) RNA viruses belonging to the order Mononegavirales are highly diversified eukaryotic viruses including significant human pathogens, such as rabies, measles, Nipah, and Ebola. Elucidation of their unique strategies to replicate in eukaryotic cells is crucial to aid in developing anti-NNS RNA viral agents. Over the...
Ogino, Tomoaki Green, Todd J.
Published in
Viruses
Rabies virus (RABV) is a causative agent of a fatal neurological disease in humans and animals. The large (L) protein of RABV is a multifunctional RNA-dependent RNA polymerase, which is one of the most attractive targets for developing antiviral agents. A remarkable homology of the RABV L protein to a counterpart in vesicular stomatitis virus, a we...
