Binici, Burcu Rattray, Zahra Zinger, Assaf Perrie, Yvonne
Published in
Journal of controlled release : official journal of the Controlled Release Society
Ionizable lipids are widely recognized as the crucial component of lipid nanoparticles (LNPs). They enable mRNA encapsulation, shield it from enzymatic degradation, facilitate cellular uptake, and foster its cytosolic release for subsequent translation into proteins. In addition, PEGylated lipids are added to stabilize the particles in storage and ...
niazi, sarfaraz k.
mRNA technology can replace the expensive recombinant technology for every type of protein, making biological drugs more affordable. It can also expedite the entry of new biological drugs, and copies of approved mRNA products can be treated as generic or biosimilar products due to their chemical nature. The introduction of hundreds of new protein d...
zhang, rui chen, jing zhang, yongbing xiumei, lu cheng, chunyan li, ji lou, qunfeng wang, yuhui qian, chuntao
Gummy stem blight (GSB), caused by Stagonosporopsis cucurbitacearum (Didymella bryoniae), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of melon against S. cucurbitacearum remains poorly understood. In this study, by employing electron microscopy and transcriptome sequencing, we investigated the cellular...
Bounas, Anastasios Talioura, Artemis Komini, Chrysoula Toli, Elisavet-Aspasia Sotiropoulos, Konstantinos Barboutis, Christos
Published in
Journal of experimental zoology. Part A, Ecological and integrative physiology
Migratory birds undertake long journeys across continents to reach breeding habitats with abundant resources. These migrations are essential for their survival and are shaped by a complex interplay of physiological adaptations, behavioral cues, and gene expression patterns. Central to migration are stopovers, critical resting points where birds rep...
zhang, rui peng, siyuan zhang, xuejuan huang, zhengwei pan, xin
Objective: The objective of this study was to explore the possibility of treating heart failure in rats by delivering mRNA of 24-dehydrocholesterol reductase (DHCR24) into the body through lipid nanoparticles (LNPs). Methods: We established a heart failure rat model using doxorubicin. The experiment was divided into blank, model, mRNA stock solutio...
saldes, erick b. erdmier, alexandra velpula, jai koeltzow, timothy e. zhu, michael x. asuthkar, swapna
Background/Objectives: Transient Receptor Potential Melastatin 8 (TRPM8) is a non-selective, Ca2+-permeable cation channel involved in thermoregulation and other physiological processes, such as basal tear secretion, cell differentiation, and insulin homeostasis. The activation and deactivation of TRPM8 occur through genetic modifications, channel ...
chentoufi, aziz a. ulmer, jeffrey b. benmohamed, lbachir
The COVID-19 pandemic, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is in its sixth year and is being maintained by the inability of current spike-alone-based COVID-19 vaccines to prevent transmission leading to the continuous emergence of variants and sub-variants of concern (VOCs). This underscores the critical need...
dawar, pranav adhikari, indra mandal, swarupa nanda jayee, bhumika
RNA metabolism is focused on RNA molecules and encompasses all the crucial processes an RNA molecule may or will undergo throughout its life cycle. It is an essential cellular process that allows all cells to function effectively. The transcriptomic landscape of a cell is shaped by the processes such as RNA biosynthesis, maturation (RNA processing,...
Mullineaux, Conrad W. Wang, Kexin Mahbub, Moontaha
Published in
Frontiers in Photobiology
The abundance and subcellular location of specific mRNA molecules can give rich information on bacterial cell biology and gene expression at the single-cell level. We have been using RNA Fluorescent in situ Hybridization (RNA-FISH) to probe for specific mRNA species in both unicellular and filamentous cyanobacteria. We have shown that the technique...
Mikec, Špela
Debelost, ki je posledica kompleksne etiologije zaradi okoljskih in genetskih dejavnikov, predstavlja velik izziv v raziskavah in zdravstvu. Nekateri genetski dejavniki, ki prispevajo k debelosti in še niso bili podrobno raziskani, vključujejo poliadenilacijo mRNA (PA), ključen proces pri zorenju in uravnavanju mRNA. Geni z več mesti PA lahko zarad...
