Drouot, Laurent Hantz, Sébastien Jouen, Fabienne Velay, Aurélie Lamia, Bouchra Veber, Benoit Sibilia, Jean Lotellier, Marlène Candon, Sophie Alain, Sophie
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Published in
Frontiers in Microbiology
Despite efforts to develop anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody (Ab) immunoassays, reliable serological methods are still needed. We developed a multiplex addressable laser bead immunoassay (ALBIA) to detect and quantify anti-Spike S1 and nucleocapsid N Abs. Recombinant S1 and N proteins were bound to fluoresce...
Car, Helena Karahan, Gonca E Dreyer, Geertje J Brand-Schaaf, Simone H de Vries, Aiko P J van Kooten, Cees Kramer, Cynthia S M Roelen, Dave L Claas, Frans H J Heidt, Sebastiaan
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Published in
HLA
Human leukocyte antigen (HLA) antibodies are induced by pregnancy, transfusion, or transplantation. Serum from transplant recipients is regularly screened for IgG HLA antibodies because of their clinical relevance for transplant outcome. While other isotypes of HLA antibodies, such as IgA may also contribute to the alloimmune response, validated de...
Sailleau, Corinne Dumarest, Marine Vanhomwegen, Jessica Delaplace, Manon Caro, Valerie Kwasiborski, Aurélia Hourdel, Véronique Chevaillier, Patrick Barbarino, Alix Comtet, Loic
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Published in
Transboundary and emerging diseases
After its first description in Wuhan (China), SARS-CoV-2 the agent of coronavirus disease 2019 (COVID-19) rapidly spread worldwide. Previous studies suggested that pets could be susceptible to SARS-CoV-2. Here, we investigated the putative infection by SARS-CoV-2 in 22 cats and 11 dogs from owners previously infected or suspected of being infected ...
Méhul, Bruno Ménigot, Corinne Fogel, Paul Seraidaris, Alexia Genette, Alexandre Pascual, Thierry Duvic, Madeleine Voegel, Johannes J
Published in
Experimental dermatology
Stratum corneum collected by tape stripping from 10 and 24 subjects with cutaneous T-cell lymphomas (CTCL) or psoriasis, respectively, were compared using quantitative label-free mass spectrometry analysis. A non-supervised statistical analysis (Posneg NMF) based on 352 differentially expressed proteins in both CTCL and psoriasis samples was able t...
Augello, Catherine J Noll, Jessica M Distel, Timothy J Wainright, Jolita D Stout, Charles E Ford, Byron D
Published in
Brain research
The use of blood biomarkers for stroke has been long considered an excellent method to determine the occurrence, timing, subtype, and severity of stroke. In this study, venous blood was obtained from ischemic stroke patients after stroke onset and compared with age and sex-matched controls. We used a multiplex panel of 37 inflammatory molecules, an...
Karahan, Gonca E Claas, Frans H J Heidt, Sebastiaan
Published in
Transplant international : official journal of the European Society for Organ Transplantation
Luminex single antigen bead assays revolutionized human leukocyte antigen (HLA) antibody detection owing to their superior sensitivity compared to conventional methods. Nevertheless, the advent of higher sensitivity came at the expense of difficulty in clinical decision-making, since not all luminex detectable antibodies are clinically relevant. Th...
Chowdhry, Mohit Makroo, Raj Nath Kakkar, Brinda Thakur, Yogita Kumar, Manoj Singh, Mandhata
Published in
Asian Journal of Transfusion Science
Transplant recipients are always at a risk of developing anti-human leukocyte antigen (HLA) antibodies due to prior sensitizing events such as blood transfusions, multiple pregnancies, or transplantation. Unexpected positive outcomes can be seen in complement dependent cytotoxicity (CDC) based assays due to underlying autoimmune disorders or pharma...
Rimington, Tracy L Hodge, Emily Billington, Charlotte K Bhaker, Sangita K C, Binaya Kilty, Iain Jelinsky, Scott Hall, Ian P Sayers, Ian
Published in
F1000Research
Airway inflammation is a feature of many respiratory diseases and there is a need for newer, more effective anti-inflammatory compounds. The aim of this study was to develop an ex vivo human lung explant model which can be used to help study the mechanisms underlying inflammatory responses and which can provide a tool to aid drug discovery for infl...
Creaney, Jenette Dick, Ian M Musk, A W Bill Olsen, Nola J Robinson, Bruce W S
Published in
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
The asbestos induced cancer malignant mesothelioma (MM) is difficult to diagnose and has a poor prognosis. MM is an immunological cancer, therefore autoantibodies may be suitable biomarkers and associated with prognosis. We used Protoarray(®) microarrays to determine immune responses to 8798 antigens in 10 MM and 10 asbestos exposed controls and de...
Garrigan, Charles Han, Jennifer Tolomeo, Pam Johnson, Katherine J Master, Stephen R Lautenbach, Ebbing Nachamkin, Irving
Published in
American journal of translational research
Research use only (RUO) assays do not undergo a validation process similar to test kits used for clinical purposes. Several studies have suggested that RUO assays need to be validated prior to use in any research studies. We evaluated a research use only Luminex platform based assay for measuring serum procalcitonin levels (Bio-Plex ProTM Human Acu...
