Claus, Maren Pieris, Naomi Urlaub, Doris Bröde, Peter Schaaf, Bernhard Durak, Deniz Renken, Frank Watzl, Carsten
Published in
Frontiers in Cellular and Infection Microbiology
The analysis of immunological parameters during the course of a SARS-CoV-2 infection is of great importance, both to identify diagnostic markers for the risk of a severe course of COVID-19, and to better understand the role of the immune system during the infection. By using multicolor flow cytometry we compared the phenotype of Natural Killer (NK)...
Newton, Hannah S. Zhang, Jenny Donohue, Duncan Unnithan, Ragi Cedrone, Edward Xu, Jie Vermilya, Alison Malys, Tyler Clogston, Jeffrey D. Dobrovolskaia, Marina A.
...
Published in
Frontiers in Allergy
Introduction Immunophenotyping, which is the identification of immune cell subsets based on antigen expression, is an integral technique used to determine changes of cell composition and activation in various disease states or as a response to different stimuli. As nanoparticles are increasingly utilized for diagnostic and therapeutic applications,...
Parakh, Sagun Leong, Tracy L. Best, Sarah A. Poh, Ashleigh R.
Published in
Frontiers in Oncology
Ren, Yuan Jia, Congwei Chen, Miao Wang, Wei Zhang, Wei
Published in
Frontiers in Oncology
Heubeck, Alexander Savage, Adam Henderson, Katherine Roll, Charles Hernandez, Veronica Torgerson, Troy Bumol, Thomas Reading, Julian
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Immunophenotyping using high dimensional flow cytometry is a central component of human immune system multi-omic studies. We present four high parameter flow cytometry panels for deep immunophenotyping of human peripheral blood mononuclear cells (PBMC). This set of four 25+ color panels include 64 cell surface markers to resolve broad immune compar...
Diray-Arce, Joann Fourati, Slim Jayavelu, Naresh Doni Patel, Ravi Maguire, Cole Chang, Ana C Dandekar, Ravi Qi, Jingjing Lee, Brian H van Zalm, Patrick
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The IMPACC cohort, composed of >1,000 hospitalized COVID-19 participants, contains five illness trajectory groups (TGs) during acute infection (first 28 days), ranging from milder (TG1-3) to more severe disease course (TG4) and death (TG5). Here, we report deep immunophenotyping, profiling of >15,000 longitudinal blood and nasal samples from 540 pa...
Grandoni, Francesco Signorelli, Federica Martucciello, Alessandra Napolitano, Francesco De Donato, Immacolata Donniacuo, Anna Di Vuolo, Gabriele De Matteis, Giovanna Del Zotto, Genny Davis, William C
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Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Water buffalo (Bubalus bubalis) has a prominent position in the livestock industry worldwide but still suffers from limited knowledge on the mechanisms regulating the immune against infections, including brucellosis (BRC), one of the most significant neglected zoonotic diseases of livestock. Seventy-three buffalo were recruited for the study. Thirt...
Gu, Yu-Mei Liang, Xiao-Long Zhang, Yun-Gang Zhao, Hong-Ying Jin, Mu-Lan Li, Xue Lu, Jun
Published in
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
目的 探讨原发性膀胱小细胞神经内分泌癌临床病理特征、免疫组织化学特点、诊断及与偶发前列腺癌的关系。方法 回顾性分析2013年1月至2022年9月在首都医科大学附属北京朝阳医院就诊的12例原发性膀胱小细胞神经内分泌癌患者的临床资料,经病理科2位副主任医师重新阅片后,按照2022版泌尿系统及男性生殖器官肿瘤世界卫生组织分类进行复核。通过检索电子病历及电话进行随访,随访自病理确诊开始至患者死亡或最后一次随访结束,截止日期2023年1月31日。结果 12例患者中,7例为pT3期,1例为pT4期;8例合并膀胱高级别尿路上皮癌、鳞状细胞癌等其他类型肿瘤,5例合并偶发前列腺癌。免疫组织化学染色结果显示,12例患者中,12例(100.0%)CD56阳性,10例(83.3%)Syn阳性,8例(66.7%)Cg...
Tadros, James Davis, Amanda Awoleye, Oreoluwa Vassiliou, Evros
Published in
Frontiers in Immunology
Hairy Cell Leukemia is an infrequent leukemia that can be recognized both microscopically and flow cytometrically once the patient develops symptoms. We present a case where early diagnosis was achieved using flow cytometry long before the patient became symptomatic. This was achieved by focusing on a small percentage (0.9%) of total leukocytes tha...
van der Pan, Kyra Khatri, Indu de Jager, Anniek L. Louis, Alesha Kassem, Sara Naber, Brigitta A.E. de Laat, Inge F. Hameetman, Marjolijn Comans, Suzanne E.T. Orfao, Alberto
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Published in
Frontiers in Immunology
Introduction Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the si...
