Audet, Marie-Claude Mangano, Emily N. Anisman, Hymie
Published in
Frontiers in Behavioral Neuroscience
Psychosocial stressors contribute to the pathophysiology of affective disorders and variations of cytokine functioning have been implicated in this process. The present investigation demonstrated, in mice, the impact of stressful aggressive encounters on activity levels, plasma corticosterone and cytokine concentrations, and on cytokine mRNA expres...
Puri, Deepika Koschorz, Birgit Engist, Bettina Onishi-Seebacher, Megumi Ryan, Devon Soujanya, Mamilla Montavon, Thomas
Published in
EMBO reports
Repeat element transcription plays a vital role in early embryonic development. The expression of repeats such as MERVL characterises mouse embryos at the 2-cell stage and defines a 2-cell-like cell (2CLC) population in a mouse embryonic stem cell culture. Repeat element sequences contain binding sites for numerous transcription factors. We identif...
Padilla-Mejia, Norma E Makarov, Alexandr A Barlow, Lael D Butterfield, Erin R Field, Mark C
Published in
Nucleus (Austin, Tex.)
Eukaryotic cells arose ~1.5 billion years ago, with the endomembrane system a central feature, facilitating evolution of intracellular compartments. Endomembranes include the nuclear envelope (NE) dividing the cytoplasm and nucleoplasm. The NE possesses universal features: a double lipid bilayer membrane, nuclear pore complexes (NPCs), and continui...
Qin, Weihua Stengl, Andreas Ugur, Enes Leidescher, Susanne Ryan, Joel Cardoso, M Cristina Leonhardt, Heinrich
Published in
Nucleus (Austin, Tex.)
Liquid-liquid phase separation (LLPS) mediated formation of membraneless organelles has been proposed to coordinate biological processes in space and time. Previously, the formation of phase-separated droplets was described as a unique property of HP1α. Here, we demonstrate that the positive net charge of the intrinsically disordered hinge region (...
Brand, Cara L. Levine, Mia T.
Repeat-enriched genomic regions evolve rapidly and yet support strictly conserved functions like faithful chromosome transmission and the preservation of genome integrity. The leading resolution to this paradox is that DNA repeat–packaging proteins evolve adaptively to mitigate deleterious changes in DNA repeat copy number, sequence, and organizati...
DeBose-Scarlett, Evon M. Sullivan, Beth A.
Centromeres are essential to genome inheritance, serving as the site of kinetochore assembly and coordinating chromosome segregation during cell division. Abnormal centromere function is associated with birth defects, infertility, and cancer. Normally, centromeres are assembled and maintained at the same chromosomal location. However, ectopic centr...
Sanchez, Nicholas A Kallweit, Lena M Trnka, Michael J Clemmer, Charles L Al-Sady, Bassem
Unique among metazoan repressive histone methyltransferases, G9a and GLP, which chiefly target histone 3 lysine 9 (H3K9), require dimerization for productive H3K9 mono (me1)- and dimethylation (me2) in vivo. Intriguingly, even though each enzyme can independently methylate H3K9, the predominant active form in vivo is a heterodimer of G9a and GLP. H...
Gao, Yuchen Han, Mengting Shang, Stephen Wang, Haifeng Qi, Lei S
Published in
Molecular cell
Eukaryotic chromosomes feature large regions of compact, repressed heterochromatin hallmarked by Heterochromatin Protein 1 (HP1). HP1 proteins play multi-faceted roles in shaping heterochromatin, and in cells, HP1 tethering to individual gene promoters leads to epigenetic modifications and silencing. However, emergent properties of HP1 at supranucl...
Matsuura, Ruka Nakajima, Tatsuro Ichihara, Saya Sado, Takashi
Published in
Frontiers in Cell and Developmental Biology
Non-coding Xist RNA plays an essential role in X chromosome inactivation (XCI) in female mammals. It coats the X chromosome in cis and mediates the recruitment of many proteins involved in gene silencing and heterochromatinization. The molecular basis of how Xist RNA initiates chromosomal silencing and what proteins participate in this process has ...
Schoberleitner, Ines Bauer, Ingo Huang, Anming Andreyeva, Evgeniya N Sebald, Johanna Pascher, Katharina Rieder, Dietmar Brunner, Melanie Podhraski, Valerie Oemer, Gregor
...
Published in
Cell reports
The ATP-dependent chromatin remodeling factor CHD1 is essential for the assembly of variant histone H3.3 into paternal chromatin during sperm chromatin remodeling in fertilized eggs. It remains unclear, however, if CHD1 has a similar role in normal diploid cells. Using a specifically tailored quantitative mass spectrometry approach, we show that Ch...
