Tarar, Ammar Alyami, Esmael M. Peng, Ching-An
Published in
Methods and Protocols
The limited amount of fusion protein transported into cytosol milieu has made it challenging to obtain a sufficient amount for further applications. To avoid the laborious and expensive task, T7 promoter-driving pET-30a(+) coding for chimeric gene of thymidine phosphorylase and core streptavidin as a model system was constructed and transformed int...
Cserjan-Puschmann, Monika Lingg, Nico Engele, Petra Kröß, Christina Loibl, Julian Fischer, Andreas Bacher, Florian Frank, Anna-Carina Öhlknecht, Christoph Brocard, Cécile
...
Published in
Biomolecules
Caspase-2 is the most specific protease of all caspases and therefore highly suitable as tag removal enzyme creating an authentic N-terminus of overexpressed tagged proteins of interest. The wild type human caspase-2 is a dimer of heterodimers generated by autocatalytic processing which is required for its enzymatic activity. We designed a circular...
Musarrat, Farhana Chouljenko, Vladimir Dahal, Achyut Nabi, Rafiq Chouljenko, Tamara Jois, Seetharama D Kousoulas, Konstantin G
Published in
Journal of medical virology
Severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) is the causative agent of the coronavirus disease-2019 (COVID-19) pandemic. Coronaviruses enter cells via fusion of the viral envelope with the plasma membrane and/or via fusion of the viral envelope with endosomal membranes after virion endocytosis. The spike (S) glycoprotein is a major ...
Fischer, Kerstin Groschup, Martin H. Diederich, Sandra
Published in
Cells
Endocytosis plays a particular role in the proteolytic activation of highly pathogenic henipaviruses Hendra (HeV) and Nipah virus (NiV) fusion (F) protein precursors. These proteins require endocytic uptake from the cell surface to be cleaved by cellular proteases within the endosomal compartment, followed by recycling to the plasma membrane for in...
Jašovič, Vita
V celični in molekularni biologiji pogosto uporabljamo poročevalne proteine, s katerimi označujemo strukture v celicah ali tkivih. Gre pravzaprav za fluorokrome, pri katerih je ob osvetljevanju značilen pojav fluorescence, ko molekula absorbira svetlobo določene valovne dolžine in nato izseva svetlobo nižje energije, torej daljše valovne dolžine. Z...
Xu, Feng Zhou, Huifen Wu, Man Zhang, Hong Zhang, Yixian Zhao, Qingbin Brown, Robert Gong, Da-Wei Miao, Lining
Published in
Bioscience Reports
Endotoxemia-induced acute kidney injury (AKI) is a common clinical condition that lacks effective treatments. Elabela (ELA) is a recently discovered kidney peptide hormone, encoded by the gene apela , and has been reported to improve cardio-renal outcomes in sepsis. However, ELA is a small peptide and is largely unsuitable for clinical use because ...
Nakano, Ayana Hirata, Isao Pham, Binh Vinh Shakya, Ajay Tanimoto, Kotaro Kato, Koichi
Published in
Journal of biomaterials science. Polymer edition
In search for peptide motifs that allow us to efficiently tether fusion proteins onto polymer surfaces, we designed a KLKLKLKLKL (KL5) decapeptide in which basic and hydrophobic amino acids are alternately linked. By means of genetic engineering technology together with a bacterial expression system, the KL5 fusions of epidermal growth factor (EGF)...
Akimov, Sergey A. Kondrashov, Oleg V. Zimmerberg, Joshua Batishchev, Oleg V.
Published in
International Journal of Molecular Sciences
Enveloped viruses include the most dangerous human and animal pathogens, in particular coronavirus, influenza virus, and human immunodeficiency virus (HIV). For these viruses, receptor binding and entry are accomplished by a single viral envelope protein (termed the fusion protein), the structural changes of which trigger the remodeling and merger ...
Belsare, Ketaki D. Ruff, Anna Joëlle Martinez, Ronny Schwaneberg, Ulrich
Published in
Biological Chemistry
Cytochrome P450s are an important group of enzymes catalyzing hydroxylation, and epoxidations reactions. In this work we describe the characterization of the CinA–CinC fusion enzyme system of a previously reported P450 using genetically fused heme (CinA) and FMN (CinC) enzyme domains from Citrobacter braaki. We observed that mixing individually ina...
LIU, Yu-Pin CHANG, Chiu-Yen LEE, Fan CHIOU, Chwei-Jang TSAI, Hsiang-Jung
Published in
The Journal of Veterinary Medical Science
Avian paramyxovirus 1 (APMV-1), synonymous with Newcastle disease virus (NDV), is a worldwide viral agent that infects various avian species and responsible for outbreaks of Newcastle disease. In this study, 40 APMV-1 isolates collected from poultry, migratory birds, and resident birds during 2010–2018 in Taiwan were characterized genetically. Our ...