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Efficient Expression of Soluble Recombinant Protein Fused with Core-Streptavidin in Bacterial Strain with T7 Expression ...

Published in Methods and Protocols

The limited amount of fusion protein transported into cytosol milieu has made it challenging to obtain a sufficient amount for further applications. To avoid the laborious and expensive task, T7 promoter-driving pET-30a(+) coding for chimeric gene of thymidine phosphorylase and core streptavidin as a model system was constructed and transformed int...

Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli , P...

Published in Biomolecules

Caspase-2 is the most specific protease of all caspases and therefore highly suitable as tag removal enzyme creating an authentic N-terminus of overexpressed tagged proteins of interest. The wild type human caspase-2 is a dimer of heterodimers generated by autocatalytic processing which is required for its enzymatic activity. We designed a circular...

Importance of Endocytosis for the Biological Activity of Cedar Virus Fusion Protein

Published in Cells

Endocytosis plays a particular role in the proteolytic activation of highly pathogenic henipaviruses Hendra (HeV) and Nipah virus (NiV) fusion (F) protein precursors. These proteins require endocytic uptake from the cell surface to be cleaved by cellular proteases within the endosomal compartment, followed by recycling to the plasma membrane for in...

Priprava rdečega fluorescenčnega proteina z N-končnim imunoglobulin-vezavnim peptidom

V celični in molekularni biologiji pogosto uporabljamo poročevalne proteine, s katerimi označujemo strukture v celicah ali tkivih. Gre pravzaprav za fluorokrome, pri katerih je ob osvetljevanju značilen pojav fluorescence, ko molekula absorbira svetlobo določene valovne dolžine in nato izseva svetlobo nižje energije, torej daljše valovne dolžine. Z...

Fc-Elabela fusion protein attenuates lipopolysaccharide-induced kidney injury in mice

Published in Bioscience Reports

Endotoxemia-induced acute kidney injury (AKI) is a common clinical condition that lacks effective treatments. Elabela (ELA) is a recently discovered kidney peptide hormone, encoded by the gene apela , and has been reported to improve cardio-renal outcomes in sepsis. However, ELA is a small peptide and is largely unsuitable for clinical use because ...

Ectodomain Pulling Combines with Fusion Peptide Inserting to Provide Cooperative Fusion for Influenza Virus and HIV

Published in International Journal of Molecular Sciences

Enveloped viruses include the most dangerous human and animal pathogens, in particular coronavirus, influenza virus, and human immunodeficiency virus (HIV). For these viruses, receptor binding and entry are accomplished by a single viral envelope protein (termed the fusion protein), the structural changes of which trigger the remodeling and merger ...

Insights on intermolecular FMN-heme domain interaction and the role of linker length in cytochrome P450cin fusion protei...

Published in Biological Chemistry

Cytochrome P450s are an important group of enzymes catalyzing hydroxylation, and epoxidations reactions. In this work we describe the characterization of the CinA–CinC fusion enzyme system of a previously reported P450 using genetically fused heme (CinA) and FMN (CinC) enzyme domains from Citrobacter braaki. We observed that mixing individually ina...

Phylogenetic analysis of avian paramyxoviruses 1 isolated in Taiwan from 2010 to 2018 and evidence for their intercontin...

Published in The Journal of Veterinary Medical Science

Avian paramyxovirus 1 (APMV-1), synonymous with Newcastle disease virus (NDV), is a worldwide viral agent that infects various avian species and responsible for outbreaks of Newcastle disease. In this study, 40 APMV-1 isolates collected from poultry, migratory birds, and resident birds during 2010–2018 in Taiwan were characterized genetically. Our ...