Hoppe, Berthold Schwedler, Christian Haibel, Hildrun Verba, Maryna Proft, Fabian Protopopov, Mikhail Heuft, Hans-Gert Rios Rodriguez, Valeria Edelmann, Anke Rudwaleit, Martin
...
Published in
International journal of molecular sciences
Coagulation and fibrinolysis are interrelated with the expression of vascular endothelial growth factor (VEGF), which frequently is increased in axial spondyloarthritis (axSpA). We tested whether (i) α2-antiplasmin (A2AP) Arg6Trp, (ii) fibrinogen, factor XIII A-subunit or B-subunit genotypes are associated with VEGF levels and assessed whether the ...
Stieler, Martin Büchold, Christian Schmitt, Marisa Heine, Andreas Hils, Martin Pasternack, Ralf Klebe, Gerhard
Published in
Chemmedchem
Life can be so easy : Blood coagulation factor XIII represents a promising target for safer anticoagulants. A crystal structure of blood coagulation factor XIII in complex with a methyl thiazole serves as starting point to address comfortably a novel transient hydrophobic pocket leading to a significantly improvement in binding affinity.
Bouttefroy, Séverine Meunier, Sandrine Milien, Vanessa Boucekine, Mohamed Chamouni, Pierre Desprez, Dominique Harroche, Annie Hochart, Audrey Thiercelin-Legrand, Marie Françoise Wibaut, Bénédicte
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Published in
British journal of haematology
This FranceCoag network study assessed 33 patients with congenital factor XIII (FXIII) deficiency presenting FXIII levels
Orosz, Zsuzsanna Z. Bárdos, Helga Shemirani, Amir H. Beke Debreceni, Ildikó Lassila, Riitta Riikonen, Antti S. Kremer Hovinga, Johanna A. Seiler, Theo G. van Dorland, Hendrika A. Schroeder, Verena
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Published in
International Journal of Molecular Sciences
Cellular factor XIII (cFXIII, FXIII-A2), a transglutaminase, has been demonstrated in a few cell types. Its main function is to cross-link proteins by isopeptide bonds. Here, we investigated the presence of cFXIII in cells of human cornea. Tissue sections of the cornea were immunostained for FXIII-A in combination with staining for CD34 antigen or ...
Protopopova, Anna D Ramirez, Andrea Klinov, Dmitry V Litvinov, Rustem I Weisel, John W
Published in
Journal of thrombosis and haemostasis : JTH
Essentials Factor XIII is a heterotetramer with 2 catalytic A subunits and 2 non-catalytic B subunits. Structure of active and inactive factor XIII was studied with atomic force microscopy. Inactive factor XIII is made of an A2 globule and 2 flexible B subunits extending from it. Activated factor XIII separates into a B2 homodimer and 2 monomeric a...
Schmitt, Lauren R. Henderson, Rachel Barrett, Alexander Darula, Zsuzsanna Issaian, Aaron D'Alessandro, Angelo Clendenen, Nathan Hansen, Kirk C.
Published in
The Journal of Biological Chemistry
The roles of factor XIIIa–specific cross-links in thrombus formation, regression, or probability for embolization are largely unknown. A molecular understanding of fibrin architecture at the level of these cross-links could inform the development of therapeutic strategies to prevent the sequelae of thromboembolism. Here, we present an MS-based meth...
Top, Oguz Geisen, Ulrich Decker, Eva L Reski, Ralf
Published in
Frontiers in plant science
The use of plants as production platforms for pharmaceutical proteins has been on the rise for the past two decades. The first marketed plant-made pharmaceutical, taliglucerase alfa against Gaucher's disease produced in carrot cells by Pfizer/Protalix Biotherapeutics, was approved by the US Food and Drug Administration (FDA) in 2012. The advantages...
Zhuang, Zhen W Huang, Yang Ju, Rong Maxfield, Mark W Ren, Yongming Wang, Xiangning Wang, Xinlu Stacy, Mitchel R Hwa, John
Published in
Theranostics
Coronary microvascular disease (MVD) remains a major clinical problem due to limited mechanistic understanding and a challenging diagnosis. In the present study we evaluated the utility of targeted imaging of active factor XIII (FXIII) for detection of coronary MVD associated with thrombus. We hypothesized that a high specificity and sensitivity FX...
Kotova, Yana N. Podoplelova, Nadezhda A. Obydennyy, Sergey Kostanova, Elizaveta A. Ryabykh, Alexander A. Demyanova, Aleksandra S. Biriukova, Maria Rosenfeld, Mark A. Sokolov, Alexey Chambost, Hervé
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Factor XIIIa (fXIIIa) is a transglutaminase that plays a crucial role in fibrin clot stabilization and regulation of fibrinolysis. It is known to bind to procoagulant platelets. In contrast, the zymogen fXIII interaction with platelets is not well characterized. We investigated the interaction of zymogen fXIII with activated platelet subpopulations...
Mitchell, J L Mutch, N J
Published in
Journal of thrombosis and haemostasis : JTH
Essentials Plasma Factor XIII, a heterodimer of A and B subunits FXIIIA2 B2 , is a transglutaminase enzyme with a well-established role in haemostasis. Cells of bone marrow and mesenchymal lineage express the FXIII-A gene (F13A1) that encodes the cellular form of the transglutaminase, a homodimer of the A subunits, FXIII-A. FXIII-A was presumed to ...
