Vukotic, Goran Obradovic, Mina Novovic, Katarina Di Luca, Mariagrazia Jovcic, Branko Fira, Djordje Neve, Horst Kojic, Milan McAuliffe, Olivia
Published in
Frontiers in Medicine
Acinetobacter baumannii is a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to tre...
Wang, Can Li, Puyuan Zhu, Yang Huang, Yong Gao, Mingming Yuan, Xin Niu, Wenkai Liu, Huiying Fan, Hang Qin, Yanhong
...
Published in
Frontiers in microbiology
The rapid expansion of Acinetobacter baumannii clinical isolates exhibiting resistance to most or all available antibiotics is a global concern. Current treatments for infections caused by this bacterium have become less effective, and the need to explore new alternative therapies is urgent. Depolymerases derived from phages are emerging as attract...
Oliveira, Hugo; Pinto, Graca; Mendes, Bruna; Dias, Oscar; Hendrix, Hanne; 99296; Akturk, Ergun; Noben, Jean-Paul; Gawor, Jan; Lobocka, Malgorzata; Lavigne, Rob; 24787;
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Providencia stuartii is emerging as a significant drug-resistant nosocomial pathogen, which encourages the search for alternative therapies. Here, we have isolated Providencia stuartii phage Stuart, a novel podovirus infecting multidrug-resistant hospital isolates of this bacterium. Phage Stuart is a proposed member of a new Autographivirinae subfa...
Knirel, Y. A. Shneider, M. M. Popova, A. V. Kasimova, A. A. Senchenkova, S. N. Shashkov, A. S. Chizhov, A. O.
Published in
Biochemistry (Moscow)
AbstractAerobic gram-negative bacterium Acinetobacter baumannii has recently become one of the most relevant pathogens associated with hospital-acquired infections worldwide. A. baumannii produces a capsule around the cell, which represents a thick viscous layer of structurally variable capsular polysaccharide (CPS). The capsule protects the bacter...
Ru, Jiakang Huo, Yixin Yang, Yu
Published in
Frontiers in Microbiology
A growing accumulation of plastic wastes has become a severe environmental and social issue. It is urgent to develop innovative approaches for the disposal of plastic wastes. In recent years, reports on biodegradation of synthetic plastics by microorganisms or enzymes have sprung up, and these offer a possibility to develop biological treatment tec...
Lee, Seung Hwan Lee, Sang Yup
Published in
Journal of microbiology and biotechnology
We have expressed extracellular poly(3-hydroxybutyrate) (PHB) depolymerase of Ralstonia pickettii T1 on the Escherichia coli surface using Pseudomonas OprF protein as a fusion partner by C-terminal deletion-fusion strategy. Surface display of depolymerase was confirmed by flow cytometry, immunofluorescence microscopy and whole cell hydrolase activi...
Knecht, Leandra E. Veljkovic, Marjan Fieseler, Lars
Published in
Frontiers in Microbiology
Bacteriophages of the Podoviridae family often exhibit so-called depolymerases as structural components of the virion. These enzymes appear as tail spike proteins (TSPs). After specific binding to capsular polysaccharides (CPS), exopolysaccharides (EPS) or lipopolysaccharide (LPS) of the host bacteria, polysaccharide-repeating units are specificall...
Wu, Yunqiang Wang, Rui Xu, Mengsha Liu, Yanan Zhu, Xianchao Qiu, Jiangfeng Liu, Qiming He, Ping Li, Qingtian
Published in
Frontiers in microbiology
The increasing prevalence of infections caused by multidrug-resistant Klebsiella pneumoniae necessitates the development of alternative therapies. Here, we isolated, characterized, and sequenced a K. pneumoniae bacteriophage (SH-KP152226) that specifically infects and lyses K. pneumoniae capsular type K47. The phage SH-KP152226 contains a genome of...
Łątka, Agnieszka Leiman, Petr G Drulis-Kawa, Zuzanna Briers, Yves
Klebsiella pneumoniae carries a thick polysaccharide capsule. This highly variable chemical structure plays an important role in its virulence. Many Klebsiella bacteriophages recognize this capsule with a receptor binding protein (RBP) that contains a depolymerase domain. This domain degrades the capsule to initiate phage infection. RBPs are highly...
Oliveira, Hugo; Costa, Ana Rita; Ferreira, Alice; Konstantinides, Nico; Santos, Silvio B; Boon, Maarten; 105361; Noben, Jean-Paul; Lavigne, Rob; 24787; Azeredo, Joana;
Acinetobacter baumannii is an important pathogen causative of health care-associated infections and is able to rapidly develop resistance to all known antibiotics, including colistin. As an alternative therapeutic agent, we have isolated a novel myovirus (vB_AbaM_B9) which specifically infects and makes lysis from without in strains of the K45 and ...
