Cisneros, Irma E. Ghorpade, Anuja Borgmann, Kathleen
Published in
Frontiers in Neurology
Methamphetamine (METH) use, referred to as methamphetamine use disorder (MUD), results in neurocognitive decline, a characteristic shared with HIV-associated neurocognitive disorders (HAND). MUD exacerbates HAND partly through glutamate dysregulation. Astrocyte excitatory amino acid transporter (EAAT)-2 is responsible for >90% of glutamate uptake f...
Ávila-Mendoza, José Subramani, Arasakumar Denver, Robert J.
Published in
Frontiers in Molecular Neuroscience
Krüppel-like factors (KLFs) are zinc finger transcription factors implicated in diverse biological processes, including differentiation of neural cells. The ability of mammalian neurons to elongate axons decreases during postnatal development in parallel with a decrease in cAMP, and increase in expression of several Klf genes. The paralogous KLFs 9...
Paramonov, Valeriy M. Sahlgren, Cecilia Rivero-Müller, Adolfo Pulliainen, Arto T.
Published in
ACS Sensors
Detection of pertussis toxin (PTX) activity is instrumental for the development and manufacturing of pertussis vaccines. These quality and safety measures require thousands of mice annually. Here, we describe I nterference in G α i -mediated S ignal T ransduction (iGIST), an animal-free kinetic bioassay for detection of PTX, by measuring its effect...
Wasner, Heinrich K
Published in
IUBMB life
While searching for a counterpart to cyclic AMP, a new compound was found to inhibit adenylate cyclase. It was identified as prostaglandyl-(15-4')-myo-inositol (1':2'-cyclic)-phosphate (cyclic PIP). The substrates for its biosynthesis are prostaglandin E (PGE) and the novel inositol phosphate, guanosine diphospho-4-myo-inositol 1:2-cyclic phosphate...
Yarwood, Stephen John
Published in
Cells
The cyclic nucleotides 3′,5′-adenosine monophosphate (cyclic AMP) signalling system underlies the control of many biological events and disease processes in man. Cyclic AMP is synthesised by adenylate cyclase (AC) enzymes in order to activate effector proteins and it is then degraded by phosphodiesterase (PDE) enzymes. Research in recent years has ...
Agarwal, Shalini Rath, Pragyan Parimita Anand, Gaurav Gourinath, Samudrala
Published in
Frontiers in Cellular and Infection Microbiology
Second messenger signaling controls a surprisingly diverse range of processes in several eukaryotic pathogens. Molecular machinery and pathways involving these messengers thus hold tremendous opportunities for therapeutic interventions. Relative to Ca2+ signaling, the knowledge of a crucial second messenger cyclic AMP (cAMP) and its signaling pathw...
Drougat, Ludivine Settas, Nikolaos Ronchi, Cristina L. Bathon, Kerstin Calebiro, Davide Maria, Andrea Gutierrez Haydar, Sara Voutetakis, Antonios London, Edra Faucz, Fabio R.
...
Published in
Genetics in Medicine
PurposeProtein kinase A (PKA) subunit defects (in PRKAR1A and PRKACA) are known to contribute to adrenal tumor pathogenesis. We studied the PRKAR1B gene for any genetic changes in bilateral adrenocortical hyperplasia (BAH) and cortisol-producing adrenal adenomas (CPA).MethodsExome sequencing and PRKAR1B copy-number variant (CNV) analysis were perfo...
Lannoy, Morgane Valluru, Manoj K Chang, Lijun Abdela-Ali, Fatima Peters, Dorien J M Streets, Andrew J Ong, Albert C M
Published in
Kidney international
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a major cause of end-stage kidney disease in man. The central role of cyclic adenosine monophosphate (cAMP) in ADPKD pathogenesis has been confirmed by numerous studies including positive clinical trial data. Here, we investigated the potential role of another major regulator of renal cAMP, pr...
Porro, Alessandro Thiel, Gerhard Moroni, Anna Saponaro, Andrea
Published in
Frontiers in Physiology
Direct regulation of the pacemaker “funny” current (If) by cyclic AMP (cAMP) underlies heart rate modulation by the autonomic nervous system. At the molecular level, cAMP activates hyperpolarization-activated cyclic nucleotide-gated (HCN) channels that drive If in sinoatrial node (SAN) myocytes. Even though HCN channel genes were identified more th...
Giacomelli, Elisa Meraviglia, Viviana Campostrini, Giulia Cochrane, Amy Cao, Xu van Helden, Ruben W.J. Krotenberg Garcia, Ana Mircea, Maria Kostidis, Sarantos Davis, Richard P.
...
Published in
Cell Stem Cell
Orlova, Bellin, Mummery, and colleagues combined three hiPSC-derived cardiac cell types in 3D microtissues. Cardiomyocytes matured structurally and functionally. Replacing healthy hiPSC-cardiac fibroblasts with patient fibroblasts recapitulated aspects of arrhythmogenic cardiomyopathy. Single-cell transcriptomics, electrophysiology, metabolomics, a...
