Nguyen, Tyler Al-Juboori, Mohammed Haider Walerstein, Jakub Xiong, Wenhui Jin, Xiaoming
Published in
Journal of neurotrauma
Although mild traumatic brain injury (mTBI) accounts for the majority of TBI patients, the effects and cellular and molecular mechanisms of mTBI on cortical neural circuits are still not well understood. Given the transient and non-specific functional deficits after mTBI, it is important to understand whether mTBI causes functional deficits of the ...
Iannone, Andrew F De Marco García, Natalia V
Published in
Neuroscience
Across mammalian species, patterned activity in neural populations is a prominent feature of developing sensory cortices. Numerous studies have long appreciated the diversity of these patterns, characterizing their differences in spatial and temporal dynamics. In the murine somatosensory cortex, neuronal co-activation is thought to guide the format...
Dzyubenko, Egor Prazuch, Wojciech Pillath-Eilers, Matthias Polanska, Joanna Hermann, Dirk M.
Published in
Frontiers in Cellular Neuroscience
Astrocytic networks are critically involved in regulating the activity of neuronal networks. However, a comprehensive and ready-to-use data analysis tool for investigating functional interactions between the astrocytes is missing. We developed the novel software package named “Astral” to analyse intercellular communication in astrocytic networks ba...
Lohr, Christian Beiersdorfer, Antonia Fischer, Timo Hirnet, Daniela Rotermund, Natalie Sauer, Jessica Schulz, Kristina Gee, Christine E.
Published in
Frontiers in Cellular Neuroscience
Ca2+ imaging is the most frequently used technique to study glial cell physiology. While chemical Ca2+ indicators served to visualize and measure changes in glial cell cytosolic Ca2+ concentration for several decades, genetically encoded Ca2+ indicators (GECIs) have become state of the art in recent years. Great improvements have been made since th...
Nelson, Carl J. Bonner, Stephen
Published in
Frontiers in Neural Circuits
Connected networks are a fundamental structure of neurobiology. Understanding these networks will help us elucidate the neural mechanisms of computation. Mathematically speaking these networks are “graphs”—structures containing objects that are connected. In neuroscience, the objects could be regions of the brain, e.g., fMRI data, or be individual ...
Bjørnstad, Daniel M. Åbjørsbråten, Knut S. Hennestad, Eivind Cunen, Céline Hermansen, Gudmund Horn Bojarskaite, Laura Pettersen, Klas H. Vervaeke, Koen Enger, Rune
Published in
Frontiers in Cellular Neuroscience
Imaging the intact brain of awake behaving mice without the dampening effects of anesthesia, has revealed an exceedingly rich repertoire of astrocytic Ca2+ signals. Analyzing and interpreting such complex signals pose many challenges. Traditional analyses of fluorescent changes typically rely on manually outlined static region-of-interests, but suc...
Niethard, Niels Brodt, Svenja Born, Jan
Published in
The Journal of Neuroscience
Sleep shapes cortical network activity, fostering global homeostatic downregulation of excitability while maintaining or even upregulating excitability in selected networks in a manner that supports memory consolidation. Here, we used two-photon calcium imaging of cortical layer 2/3 neurons in sleeping male mice to examine how these seemingly oppos...
Seidel, Eric Schewe, Julia Zhang, Junhui Dinh, Hoang An Forslund, Sofia K. Markó, Lajos Hellmig, Nicole Peters, Jörg Muller, Dominik N. Lifton, Richard P.
...
Published in
Proceedings of the National Academy of Sciences of the United States of America
Gain-of-function mutations in the CACNA1H gene (encoding the T-type calcium channel CaV3.2) cause autosomal-dominant familial hyperaldosteronism type IV (FH-IV) and early-onset hypertension in humans. We used CRISPR/Cas9 to generate Cacna1h M1560V/+ knockin mice as a model of the most common FH-IV mutation, along with corresponding knockout mice ( ...
Neugornet, Austin O’Donovan, Bernadette Ortinski, Pavel Ivanovich
Published in
Frontiers in Neuroscience
Calcium imaging has gained substantial popularity as a tool to profile the activity of multiple simultaneously active cells at high spatiotemporal resolution. Among the diverse approaches to processing of Ca2+ imaging data is an often subjective decision of how to quantify baseline fluorescence or F0. We examine the effect of popular F0 determinati...
Onimaru, Hiroshi Yazawa, Itaru Takeda, Kotaro Fukushi, Isato Okada, Yasumasa
Published in
Frontiers in Physiology
It is supposed that the nucleus of the solitary tract (NTS) in the dorsal medulla includes gas sensor cells responsive to hypercapnia or hypoxia in the central nervous system. In the present study, we analyzed cellular responses to hypercapnia and hypoxia in the NTS region of newborn rat in vitro preparation. The brainstem and spinal cord were isol...
