Anthoney, Niki Tainton-Heap, Lucy Luong, Hang Notaras, Eleni Kewin, Amber B Zhao, Qiongyi Perry, Trent Batterham, Philip Shaw, Paul J van Swinderen, Bruno
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Published in
eLife
Sleep in mammals can be broadly classified into two different physiological categories: rapid eye movement (REM) sleep and slow-wave sleep (SWS), and accordingly REM and SWS are thought to achieve a different set of functions. The fruit fly Drosophila melanogaster is increasingly being used as a model to understand sleep functions, although it rema...
Wang, Camille S Monteggia, Lisa M Kavalali, Ege T
Published in
Cell reports
Calcium (Ca2+) signaling is tightly regulated within a presynaptic bouton. Here, we visualize Ca2+ signals within hippocampal presynaptic boutons using GCaMP8s tagged to synaptobrevin, a synaptic vesicle protein. We identify evoked presynaptic Ca2+ transients (ePreCTs) that derive from synchronized voltage-gated Ca2+ channel openings, spontaneous p...
Ito, Hiroto Fukatsu, Noriaki Rahaman, Sheikh Mizanur Mukai, Yasutaka Izawa, Shuntaro Ono, Daisuke Kilduff, Thomas S Yamanaka, Akihiro
Published in
Proceedings of the National Academy of Sciences of the United States of America
Narcolepsy is a sleep disorder caused by deficiency of orexin signaling. However, the neural mechanisms by which deficient orexin signaling causes the abnormal rapid eye movement (REM) sleep characteristics of narcolepsy, such as cataplexy and frequent transitions to REM states, are not fully understood. Here, we determined the activity dynamics of...
Foubert, David Cookson, Finnley Ruthazer, Edward S
Published in
Neurophotonics
The increasingly widespread use of calcium imaging to explore the nature of neuronal activity and circuits has unexpectedly revealed the ubiquitous presence and significance of astrocytic activity. Here, we present a brief review of visual system development, placing it in the context of recently identified roles of astrocytes in the modulation of ...
Patel, Niraj Ouellet, Vincent Paquet-Mercier, François Chetoui, Nizar Bélanger, Erik Paquet, Marie-Eve Godin, Antoine G. Marquet, Pierre
Published in
Frontiers in Neuroscience
Introduction Human induced pluripotent stem cells (iPSCs), with their ability to generate human neural cells (astrocytes and neurons) from patients, hold great promise for understanding the pathophysiology of major neuropsychiatric diseases such as schizophrenia and bipolar disorders, which includes alterations in cerebral development. Indeed, the ...
Pasarkar, Amol Kinsella, Ian Zhou, Pengcheng Wu, Melissa Pan, Daisong Fan, Jiang Lan Wang, Zhen Abdeladim, Lamiae Peterka, Darcy S Adesnik, Hillel
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Published in
bioRxiv : the preprint server for biology
A number of calcium imaging methods have been developed to monitor the activity of large populations of neurons. One particularly promising approach, Bessel imaging, captures neural activity from a volume by projecting within the imaged volume onto a single imaging plane, therefore effectively mixing signals and increasing the number of neurons ima...
Talmon, Maria Massara, Erika Quaregna, Martina De Battisti, Marta Boccafoschi, Francesca Lecchi, Giulia Puppo, Federico Bettega Cajandab, Michele A. Salamone, Stefano Bovio, Enrica
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Published in
Frontiers in Pharmacology
Bitter taste receptors are involved not only in taste perception but in various physiological functions as their anatomical location is not restricted to the gustatory system. We previously demonstrated expression and activity of the subtype hTAS2R46 in human airway smooth muscle and broncho-epithelial cells, and here we show its expression and fun...
Kourdougli, Nazim Suresh, Anand Liu, Benjamin Juarez, Pablo Lin, Ashley Chung, David T Graven Sams, Anette Gandal, Michael J Martínez-Cerdeño, Verónica Buonomano, Dean V
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Changes in the function of inhibitory interneurons (INs) during cortical development could contribute to the pathophysiology of neurodevelopmental disorders. Using all-optical in vivo approaches, we find that parvalbumin (PV) INs and their immature precursors are hypoactive and transiently decoupled from excitatory neurons in postnatal mouse somato...
Skelin Klemen, Maša Dolenšek, Jurij Križančić Bombek, Lidija Pohorec, Viljem Gosak, Marko Slak Rupnik, Marjan Stožer, Andraž
Published in
Frontiers in Endocrinology
Beta cells couple stimulation by glucose with insulin secretion and impairments in this coupling play a central role in diabetes mellitus. Cyclic adenosine monophosphate (cAMP) amplifies stimulus-secretion coupling via protein kinase A and guanine nucleotide exchange protein 2 (Epac2A). With the present research, we aimed to clarify the influence o...
Amedzrovi Agbesi, Richard Spencer, Nick Hebberd, Tim Chevalier, Nicolas R.
Gut motility undergoes a switch from myogenic to neurogenic control in late embryonic development. Here, we report on the first electrical events underlying neurogenic motility in the developing mouse, using a transgenic line expressing the GCamp6f reporter in neural crest cell derivatives. We find that both nitrergic inhibitory and cholinergic exc...
