Murashko, Oleg N. Yeh, Kun-Hai Yu, Chen-Hsin Albert Kaberdin, Vladimir R. Lin-Chao, Sue
Published in
Microbiology Spectrum
Although fluoride-containing compounds are widely used to inhibit bacterial growth, the reprogramming of gene expression underlying cellular responses to fluoride, especially under anaerobic conditions, is still poorly understood. Here, we compare the genome-wide transcriptomic profiles of E. coli grown in the absence (control) or presence (20 and ...
Grigaitis, Pranas van den Bogaard, Samira L Teusink, Bas
Published in
FEMS Yeast Research
In the article, the authors blended computational modeling with experimental measurements to identify additional energy costs for growth of Saccharomyces cerevisiae when it cannot use respiration for energy generation.
Krause, David J Hittinger, Chris Todd
Published in
Molecular biology and evolution
The amplification and diversification of genes into large multi-gene families often mark key evolutionary innovations, but this process often creates genetic redundancy that hinders functional investigations. When the model budding yeast Saccharomyces cerevisiae transitions to anaerobic growth conditions, the cell massively induces the expression o...
Vanacloig-Pedros, Elena Fisher, Kaitlin J Liu, Lisa Debrauske, Derek J Young, Megan K M Place, Michael Hittinger, Chris Todd Sato, Trey K Gasch, Audrey P
Published in
FEMS Yeast Research
This work quantifies the fitness contributions of
Dekker, Wijbrand J.C.; Jurgens, Hannes; Ortiz-Merino, Raul A.; 145799; Mooiman, Christiaan; van den Berg, Remon; Kaljouw, Astrid; Mans, Robert; Pronk, Jack T.;
While thermotolerance is an attractive trait for yeasts used in industrial ethanol production, oxygen requirements of known thermotolerant species are incompatible with process requirements. Analysis of oxygen-sufficient and oxygen-limited chemostat cultures of the facultatively fermentative, thermotolerant species Ogataea parapolymorpha showed its...
Dekker, Wijbrand J C Jürgens, Hannes Ortiz-Merino, Raúl A Mooiman, Christiaan van den Berg, Remon Kaljouw, Astrid Mans, Robert Pronk, Jack T
Published in
FEMS Yeast Research
Large oxygen requirements of the industrially relevant yeast O. parapolymorpha in oxygen-limited cultures were attributed to a necessity to reoxidize surplus NADH from biosynthetic reactions by mitochondrial respiration (‘Custers effect’).
Aranda-Díaz, Andrés Rodrigues, Cecilia Grote, Alexandra Sun, Jiawei Schreck, Carl Hallatschek, Oskar Souslov, Anton Möbius, Wolfram Huang, Kerwyn Casey
Published in
mBio
Chirality is ubiquitous in nature, with consequences at the cellular and tissue scales. As Escherichia coli colonies expand radially, an orthogonal component of growth creates a pinwheel-like pattern that can be revealed by fluorescent markers. To elucidate the mechanistic basis of this colony chirality, we investigated its link to left-handed, sin...
Song, Xuejiao Cronan, John E
Published in
Molecular microbiology
Biotin is an essential metabolic cofactor and de novo biotin biosynthetic pathways are widespread in microorganisms and plants. Biotin synthetic genes are generally found clustered into bio operons to facilitate tight regulation since biotin synthesis is a metabolically expensive process. Dethiobiotin synthetase (DTBS) catalyzes the penultimate ste...
Hobbs, Laura Allen, Leah Bias, Megan Johnson, Stephanie DeRespiris, Hannah Diallo, Chantal Bui, Loan Sun, Yvonne
Published in
Frontiers in Microbiology
Listeria monocytogenes is a Gram-positive, intracellular pathogen responsible for the highly fatal foodborne illness listeriosis. Establishing intracellular infections requires the coordinated expressions of a variety of virulence factors, such as the pore-forming toxin listeriolysin O (LLO), in response to various intra- and extracellular signals....
Wu, Chih-Hung Rismondo, Jeanine Morgan, Rhodri M L Shen, Yang Loessner, Martin J Larrouy-Maumus, Gerald Freemont, Paul S Gründling, Angelika
Published in
The Journal of biological chemistry
UTP-glucose-1-phosphate uridylyltransferases are enzymes that produce UDP-glucose from UTP and glucose-1-phosphate. In Bacillus subtilis 168, UDP-glucose is required for the decoration of wall teichoic acid (WTA) with glucose residues and the formation of glucolipids. The B. subtilis UGPase GtaB is essential for UDP-glucose production under standar...
