In the context of widespread public and political concern around the use of animals in research, we sought to examine the scientific, ethical and economic arguments around the replacement of animals with New Approach Methodologies (NAMs) and to situate this within a regulatory context. We also analyzed the extent to which animal replacement aligns ...
Published in Frontiers in Toxicology
Per- and polyfluoroalkyl substances (PFAS) have become ubiquitous environmental contaminants that have been associated with adverse pregnancy outcomes in women and experimental research models. Adverse developmental and reproductive outcomes have been investigated for relatively few PFAS, and such studies are not scalable to address the thousands o...
(1) Background: In cystic fibrosis (CF), the oral glucose tolerance test (OGTT) is recommended from 10 years old annually to screen and diagnose cystic fibrosis-related diabetes (CFRD). Alternative OGTT characteristics (glucose curve shape, time to glucose peak, one-hour glucose value, and three-hour glucose value with the new shape curve) were stu...
Firefly luciferase is susceptible to inhibition and stabilization by compounds under investigation for biological activity and toxicity. This can lead to false-positive results in in vitro cell-based assays. However, firefly luciferase remains one of the most commonly used reporter genes. Here, we evaluated isoflavonoids for inhibition of firefly l...
Published in Przegla̜d Menopauzalny = Menopause Review
The aim of the study was to assess the prevalence of menopausal symptoms, and the use of menopausal hormone therapy (MHT) and nonconventional methods of alleviating menopausal symptoms and their health benefits in peri- and postmenopausal women. A sample of 349 peri- or postmenopausal women were studied, all of whom had experienced menopausal sympt...
V laboratorijskih razmerah smo preučevali insekticidno delovanje prahov štirih invazivnih tujerodnih rastlinskih vrst (navadne amorfe [Amorpha fruticosa], velikega pajesena [Ailanthus altissima], kanadske zlate rozge [Solidago canadensis] in octovca [Rhus typhina]) v samostojni uporabi in v kombinacijah z lesnim pepelom in diatomejsko zemljo. Cilj ...
(1) Background: Three-dimensional (3D) collagen I-based skin models are commonly used in drug development and substance testing but have major drawbacks such as batch-to-batch variations and ethical concerns. Recently, synthetic nanofibrous scaffolds created by electrospinning have received increasing interest as potential alternatives due to their...
Prerequisite to any biological laboratory assay employing living animals is consideration about its necessity, feasibility, ethics and the potential harm caused during an experiment. The imperative of these thoughts has led to the formulation of the 3R-principle, which today is a pivotal scientific standard of animal experimentation worldwide. The ...
Published in Frontiers in Toxicology
Most cell culture models are static, but the cellular microenvironment in the body is dynamic. Here, we established a microfluidic-based in vitro model of human bronchial epithelial cells in which cells are stationary, but nutrient supply is dynamic, and we used this system to evaluate cellular uptake of nanoparticles. The cells were maintained in ...
Senzorična analiza je poleg fizikalno kemijske in mikrobiološke analize pomembna metoda za določanje kakovosti živil. Delimo jo na hedonske in analitične preizkuse, kamor spada tudi tradicionalna opisna analiza, ki pa je zelo zamudna in draga, zato se za ocenjevanje s potrošniki poslužujemo tako imenovanih hitrih ali alternativnih senzoričnih metod...
