Modulators of Innate Gut Immunity to Enteric Viral Infections: Murine Norovirus (MNV) as a Model
IDB Cambridge International Scholarship
First experimental assessment of protein intrinsic disorder involvement in an RNA virus natural adaptive process
Intrinsic disorder (ID) in proteins is defined as a lack of stable structure in physiological conditions. Intrinsically disordered regions (IDRs) are highly abundant in some RNA virus proteomes. Low topological constraints exerted on IDRs are expected to buffer the effect of numerous deleterious mutations and could be related to the remarkable adap...
Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume).
With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyzed by Illumina sequencing. Blast comparisons of re...
RSV hijacks cellular protein phosphatase 1 to regulate M2-1 phosphorylation and viral transcription
Respiratory syncytial virus (RSV) RNA synthesis occurs in cytoplasmic inclusion bodies (IBs) in which all the components of the viral RNA polymerase are concentrated. In this work, we show that RSV P protein recruits the essential RSV transcription factor M2-1 to IBs independently of the phosphorylation state of M2-1. We also show that M2-1 dephosp...
A novel badnavirus discovered from Betula sp. affected by birch leaf-roll disease.
In declining birches (Betula sp.) from different European stands affected by the "birch leaf-roll disease" (BLRD) a novel virus is identified by means of RNA-Seq virome analysis. The virus represents a new member in the genus Badnavirus, family Caulimoviridae, tentatively named Birch leaf roll-associated virus (BLRaV) and it is the first badnavirus...
Evidence of porcine epidemic diarrhea virus (PEDV) shedding in semen from infected specific pathogen-free boars
...
In 2013, PED emerged for the first time in the United States (US). The porcine epidemic diarrhea virus (PEDV) spread quickly throughout North America. Infection with PEDV causes watery diarrhea and up to 100% mortality in piglets, particularly for highly pathogenic non-InDel strains circulating in the US. PEDV is mainly transmitted by the fecal-ora...
Metagenomic-based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome.
...
For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus-resistant transgenic plants based on the pathogen-derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of a...
Major contribution of the RNA-binding domain of NS1 in the pathogenicity and replication potential of an avian H7N1 infl...
...
Background: Non-structural protein NS1 of influenza A viruses harbours several determinants of pathogenicity and host-range. However it is still unclear to what extent each of its two structured domains (i.e. RNA-binding domain, RBD, and effector domain, ED) contribute to its various activities. Methods: To evaluate the respective contributions of ...
Revisiting the roles of tobamovirus replicase complex proteins in viral replication and silencing suppression
Tobamoviral replicase possesses an RNA-dependent RNA polymerase (RDR) domain and is translated from genomic (g)RNA via a stop codon readthrough mechanism at a one-to-ten ratio relative to a shorter protein lacking the RDR domain. The two proteins share methyltransferase and helicase domains and form a heterodimer implicated in gRNA replication. The...
Translation-Mediated Stress Responses: Mining of Ribosome Profiling Data
Gates-Cambridge Scholarship
