Anisimov, Mikhail N Korshunova, Alena V Popov, Vladimir V Gudimchuk, Nikita B
Published in
European journal of cell biology
Microtubules are essential cytoskeletal polymers, which exhibit stochastic transitions between assembly and disassembly, known as catastrophes and rescues. Understanding of catastrophes, rescues, and their control by drugs and microtubule associated proteins (MAPs) has been informed by in vitro reconstitutions of microtubule dynamics. In such exper...
Ahmed, Jawad Sajjad, Yasar Latif, Aasia Lodhi, Mohammad Saeed Huzafa, Muhammad Situ, Chen Ahmad, Raza Shah, Muhammad Maroof Hassan, Amjad
Published in
Journal of plant research
Wall-associated kinases (WAKs) are a unique family of proteins that are predominantly localized on the plasma membrane and simultaneously bound to the cell wall. WAKs play a pivotal role in signal transduction to regulate growth, defense, and response to environmental stimuli in plants. These kinases have been identified and characterized in variou...
Reimann, Hauke Stopper, Helga Hintzsche, Henning
Published in
Archives of toxicology
Although micronuclei are well-known biomarkers of genotoxic damage, the biological consequences of micronucleus induction are only poorly understood. To further elucidate these consequences, HeLa cells stably expressing histone 2B coupled with green fluorescent protein were used for long-term live cell imaging to investigate the fate of micronuclei...
Fahmy, Maha A Hassan, Entesar E Farghaly, Ayman A Hassan, Zeinab M
Published in
Molecular biology reports
The treatment with chemotherapy may develop secondary tumors as a result of chemo genotoxicity. Sperm defects is another complication associated with chemo treatment. In this study the genotoxicity of vinblastine (VB) was estimated in both somatic and germ cells. 85 mice were taken. Four single doses of VB at 3, 4.5, 6 and 10 mg/kg and three succes...
Witta, Sandra Collins, Keagan P Ramirez, Dominique A Mannheimer, Joshua D Wittenburg, Luke A Gustafson, Daniel L
Vinblastine (VBL) is a vinca alkaloid-class cytotoxic chemotherapeutic that causes microtubule disruption and is typically used to treat hematologic malignancies. VBL is characterized by a narrow therapeutic index, with key dose-limiting toxicities being myelosuppression and neurotoxicity. Pharmacokinetics (PK) of VBL is primarily driven by ABCB1-m...
De la Garza-Salazar, Fernando Gómez-De León, Andrés Gómez-Almaguer, David Colunga-Pedraza, Perla R
Published in
Annals of hematology
Fornecker, Luc-Matthieu Lazarovici, Julien Aurer, Igor Casasnovas, René-Olivier Gac, Anne-Claire Bonnet, Christophe Bouabdallah, Krimo Feugier, Pierre Specht, Lena Molina, Lysiane
...
peer reviewed
Heydari, Fatemeh Matty, Kristina Löfstrand, Lina Ekeroth, Måns Marzban, Maryam Wigert, Ella
The purpose of this project was to develop and optimise a protocol for live-cell imaging with the Nikon Eclipse Ti2-E Live-cell Fluorescence Imaging microscope by using two types of blood cancers cell lines, K562 and L428. The clearest visualisation for dual staining of the K562 was achieved with the concentrations 0.05 μg/ml of Hoechst dye and 2.0...
Zhang, Jie Hansen, Lea G Gudich, Olga Viehrig, Konrad Lassen, Lærke MM Schrübbers, Lars Adhikari, Khem B Rubaszka, Paulina Carrasquer-Alvarez, Elena Chen, Ling
...
Monoterpene indole alkaloids (MIAs) are a diverse family of complex plant secondary metabolites with many medicinal properties, including the essential anti-cancer therapeutics vinblastine and vincristine1. As MIAs are difficult to chemically synthesize, the world's supply chain for vinblastine relies on low-yielding extraction and purification of ...
Chew, Katherine Lee, Branden van Haren, Simon D Nanishi, Etsuro O'Meara, Timothy Splaine, Jennifer B DeLeon, Maria Soni, Dheeraj Seo, Hyuk-Soo Dhe-Paganon, Sirano
...
Published in
bioRxiv : the preprint server for biology
We describe a methodology utilizing high-throughput and high-content screening for novel adjuvant candidates that was used to screen a library of ∼2,500 small molecules via a 384-well quantitative combined cytokine and flow cytometry immunoassay in primary human peripheral blood mononuclear cells (PBMCs) from 4 healthy adult study participants. Hit...
