© 2019, The Author(s), under exclusive licence to Springer Nature Limited. Innovations in teaching STEM subjects can help build critical science literacy and address global economic needs. Virology teachers and researchers are actively developing and integrating innovative educational materials for students, creating engaging teaching programs and ...
The experimental design that will be carried out to evaluate a nucleic acid quantification hypothesis determines the cost and feasibility of digital polymerase chain reaction (digital PCR) studies. Experiment design involves the calculation of the number of technical measurement replicates and the determination of the characteristics of those repli...
Published in European Journal of Hospital Pharmacy
Background The interference in the immune response induced by biological disease-modifying antirheumatic drugs (bDMARDs) increases the risk of reactivation of infections. Treatment of patients with chronic hepatitis C virus (HCV) infection and psoriasis is complex. The efficacy and safety of the new direct-acting antiviral agents (DAA) when combine...
Published in Journal of neurology, neurosurgery, and psychiatry
Published in Postgraduate medical journal
The opioid epidemic in the USA continues to worsen. Medical providers are faced with the challenge of addressing complications from opioid use disorders and associated injection drug use. Unsafe injection practices among people who inject drugs (PWID) can lead to several complications requiring acute care encounters in the emergency department and ...
Published in BMC Bioinformatics
BackgroundNext generation sequencing enables studying heterogeneous populations of viral infections. When the sequencing is done at high coverage depth (“deep sequencing”), low frequency variants can be detected. Here we present QQ-SNV (http://sourceforge.net/projects/qqsnv), a logistic regression classifier model developed for the Illumina sequenc...
Background Metagenomic analyses have been widely used in the last decade to describe viral communities in various environments or to identify the etiology of human, animal, and plant pathologies. Here, we present a simple and standardized protocol that allows for the purification and sequencing of RNA viromes from complex biological samples with an...
Published in European Journal of Hospital Pharmacy
Background Most studies focus on potential drug interactions, without considering the effect of these on the response to antiretroviral (ARV) therapy. We assess the effect of potential drug–drug interactions (pDDIs) that could have lowered the ARV concentration (pDDI-lowerARV) on HIV viral load. Methods Retrospective observational cohort study was ...
Published in Journal of clinical pathology
Based on the results, the prevalence of herpesviruses and the qualitative bacterial profiles was higher in children with leukaemia and HCMV, HHV-7 and HHV-8 were related to the use of chemotherapy. Moreover, HHV-6 was correlated with an increased bacterial community profile in patients with leukaemia (p
Published in BMJ open
Skin biopsies from local sites of herpes simplex virus 2 (HSV-2)-induced ulcers can show infiltrates of inflammatory cells several months after macroscopic healing. We hypothesise that foreskin tissue samples of asymptomatic HSV-2 seropositive men had remaining signs of inflammation at the molecular level. Even in the absence of clinical lesions, g...
