The yeast Ty1 retrotransposon integrates upstream of genes transcribed by RNA polymerase III (Pol III). Specificity of integration is mediated by an interaction between the Ty1 integrase (IN1) and Pol III, currently uncharacterized at the atomic level. We report cryo-EM structures of Pol III in complex with IN1, revealing a 16-residue segment at th...
Published in Gene
Several covalent modifications are found associated with the transcriptionally active chromatin regions constituted by the genes transcribed by RNA polymerase (pol) II. Pol III-transcribed genes code for the small, stable RNA species, which participate in many cellular processes, essential for survival. Pol III transcription is repressed under most...
Published in Transcription
Eukaryotic cells rely upon dynamic, multifaceted regulation at each step of RNA biogenesis to maintain mRNA pools and ensure normal protein synthesis. Studies in budding yeast indicate a buffering phenomenon that preserves global mRNA levels through the reciprocal balancing of RNA synthesis rates and mRNA decay. In short, changes in transcription i...
Direct multiple shooting (DMS) and direct collocation (DC) are two standard transcription methods for solving optimal control problems (OCP) in biomechanics and robotics. They have yet to be compared in terms of solution and speed. Through five examples of predictive simulations solved using five transcription methods and 100 initial guesses in the...
Published in BMC biology
The FACT complex is a conserved histone chaperone with critical roles in transcription and histone deposition. FACT is essential in pluripotent and cancer cells, but otherwise dispensable for most mammalian cell types. FACT deletion or inhibition can block induction of pluripotent stem cells, yet the mechanism through which FACT regulates cell fate...
Identifying therapeutics to delay, and potentially reverse, age-related cognitive decline is critical in light of the increased incidence of dementia-related disorders forecasted in the growing older population1. Here we show that platelet factors transfer the benefits of young blood to the ageing brain. Systemic exposure of aged male mice to a fra...
Iron (Fe) is a micronutrient that is essential for plant growth and development as well as for crop productivity and the quality of their derived products. Despite its high abundance in Earth's crust, Fe is poorly available to plants in one-third of the cultivated land. This is because at neutral to alkaline pH, Fe is mostly present in the form of ...
International audience
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