Hu, Congcong Wei, Xinlei Song, Yunhong
Published in
Enzyme and microbial technology
Phosphatases catalyze the irreversible dephosphorylation of phosphate-containing compounds, and hence can be applied as the final enzymatic step for the synthesis of various biochemicals. However, the extensive substrate spectrums of phosphatases impose a great challenge for efficient biomanufacturing. Characterization of phosphatases is therefore ...
Panwar, Varsha Sheikh, Javed Nabibaksha Dutta, Tanmay
Published in
Applied biochemistry and biotechnology
Laccases have emerged as environment-friendly multifaceted biocatalysts for diverse biotechnological applications. Here, we isolated a high molecular weight (88 kDa) extremophilic laccase (LacT) from Brevibacillus agri, with the aim to exploit its extreme characters in denim bleaching. LacT has been characterized as a thermostable, acidophilic enzy...
Kamijo, Junya Sakai, Kiyota Suzuki, Hiromitsu Suzuki, Kengo Kunitake, Emi Shimizu, Motoyuki Kato, Masashi
Published in
Food chemistry
Pectinolytic enzymes are used in diverse industrial applications. We sought to isolate a pectate lyase from Aspergillus luchuensis var. saitoi, a filamentous fungus used in traditional food and beverage preparation in Japan. The identified enzyme, named AsPelA, is orthologous to PelA from A. luchuensis mut. kawachii (AkPelA); the enzymes exhibit 99...
Satomura, Takenori Hayashi, Junji Ohshida, Tatsuya Sakuraba, Haruhiko Ohshima, Toshihisa Suye, Shin-ichiro
Published in
Extremophiles
A gene-encoding a dye-linked d-lactate dehydrogenase (Dye-DLDH) homolog was identified in the genome of the hyperthermophilic archaeon Thermoproteus tenax. The gene was expressed in Escherichia coli and the product was purified to homogeneity. The recombinant protein exhibited highly thermostable Dye-DLDH activity. To date, four types of Dye-DLDH h...
Navas, Laura Emilce Florin-Christensen, Mónica Benintende, Graciela Beatriz Zandomeni, Rubén Oreste Berretta, Marcelo Facundo
Published in
Journal of Molecular Microbiology and Biotechnology
Phospholipases are classified in different enzyme families according to the ester bond they cleave within phospholipids. The use of phospholipases in industrial processes has prompted the search for new enzymes with differential properties. A gene encoding a novel phospholipase (PLP_2.9) was identified in the genome of the thermophilic strain Therm...
Washio, Tsubasa Oikawa, Tadao
Published in
Extremophiles
We successfully expressed the l-aspartate oxidase homolog gene (accession no: OCC_06611) of Thermococcus litoralis DSM 5473 in the soluble fraction of Escherichia coli BL21 (DE3) using a pET21b vector with 6X His tag at its C-terminus. The gene product (Tl-LASPO) showed l-aspartate oxidase activity in the presence of FAD in vitro, and this report i...
Lorillière, Marion
La transcétolase thermostable de Geobacillus stearothermophilus (TKgst, EC 2.2.1.1) permet de synthétiser efficacement à haute température des cétoses à 4, 5 et 6 atomes de carbone de configuration d-thréo (3S, 4R), par formation stéréosélective d’une liaison C-C, à partir d’aldéhydes α-hydroxylés (2R) à courte chaîne. L’objectif de ces travaux est...
Kumari, Arpana Kishor, Nitin Guptasarma, Purnananda
Published in
Biotechnology Letters
ObjectiveTo examine the potential for applications of TthLAC, a monomeric (~ 53 kDa) laccase encoded by the genome of Thermus thermophilus (strain HB 27) which can be produced at low cost in Escherichia coli.ResultFunctional, thermostable and mildly alkalophilic TthLAC of high purity (> 90%) was produced through simple heating of suspended (TthLAC ...
Jensen, Torbjørn Ølshøj Pogrebnyakov, Ivan Falkenberg, Kristoffer Bach Redl, Stephanie Nielsen, Alex Toftgaard
Published in
AMB Express
Use of thermophilic organisms has a range of advantages, but the significant lack of engineering tools limits their applications. Here we show that β-galactosidase from Geobacillus stearothermophilus (BgaB) can be applicable in a range of conditions, including different temperatures and oxygen concentrations. This protein functions both as a marker...
Pek, Han Bin Lim, Pei Yu Liu, Chengcheng Lee, Dong-Yup Bi, Xuezhi Wong, Fong Tian Ow, Dave Siak-Wei
Published in
Biotechnology Letters
ObjectivesTo evaluate the secretory and cytoplasmic expression of a thermostable Thermogata maritima invertase in Lactococcus lactis.ResultsThe thermostable invertase from T. maritima was cloned with and without the USP45 secretory peptide into the pNZ8148 vector for nisin-inducible expression in L. lactis. The introduction of an USP45 secretion pe...
