An ion transporter in sperm that has features of a channel.
Published in Nature
Two-sample goodness-of-fit tests on the flat torus based on Wasserstein distance and their relevance to structural biolo...
This work is motivated by the study of local protein structure, which is defined by two variable dihedral angles that take values from probability distributions on the flat torus. Our goal is to provide the space $\mathcal{P}(\mathbb{R}^2/\mathbb{Z}^2)$ with a metric that quantifies local structural modifications due to changes in the protein seque...
Nucleotide-free structures of KIF20A illuminate atypical mechanochemistry in this kinesin-6
KIF20A is a critical kinesin for cell division and a promising anti-cancer drug target. The mechanisms underlying its cellular roles remain elusive. Interestingly, unusual coupling between the nucleotide- and microtubule-binding sites of this kinesin-6 has been reported, but little is known about how its divergent sequence leads to atypical motilit...
Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn²⁺ uptake into the Golgi apparatus
...
クライオ電子顕微鏡により、ゴルジ体の亜鉛輸送体による亜鉛輸送機構の全容を解明 細胞の亜鉛恒常性維持機構の理解に大きな進展. 京都大学プレスリリース. 2023-08-29. / Zinc ions (Zn²⁺) are vital to most cells, with the intracellular concentrations of Zn²⁺ being tightly regulated by multiple zinc transporters located at the plasma and organelle membranes. We herein present the 2.2-3.1 Å-resolution cryo-EM structures of a Golgi...
1H, 15N and 13C backbone resonance assignments of the acidic domain of the human MDM2 protein.
Published in Biomolecular NMR assignments
The human MDM2 protein regulates the tumor suppressor protein p53 by restricting its transcriptional activity and by promoting p53 degradation. MDM2 is ubiquitously expressed, with its overexpression implicated in many forms of cancer. The inhibitory effects of MDM2 on p53 have been shown to involve its N-terminal p53-binding domain and its C-termi...
A metal ion-dependent mechanism of RAD51 nucleoprotein filament disassembly
The RAD51 ATPase polymerises on single-stranded DNA to form nucleoprotein filaments (NPFs) that are critical intermediates in the reaction of Homologous Recombination. ATP binding maintains the NPF in a competent conformation for strand pairing and exchange. Once strand exchange is completed, ATP hydrolysis licenses the filament for disassembly. He...
What Rosalind Franklin truly contributed to the discovery of DNA's structure.
Published in Nature
Caractérisation structurale des protéines P et C encodées par des séquences chevauchantes chez les Orthoparamyxovirinae
La famille des Paramyxoviridae est une grande famille de virus à ARN négatif non-segmenté dont certains sont pathogènes pour l’homme ou les animaux domestiques. Leur génome de taille réduite comprend six unités de transcription, dont celle codant pour la phosphoprotéine P qui est polycistronique et permet la production, d’une part, de la phosphopro...
Collecting Data for Generating Distance Geometry Graphs for Protein Structure Determination
We summarize some ideas for converting different types of information about protein molecules into pure distance information, so that to employ a discrete distance geometry approach for the identification of potential protein conformations.
Structural and functional study of the HEV ORF3 protein by solution NMR
Hepatitis E Virus (HEV) is the most common cause of acute viral hepatitis worldwide with over 20 million infections and around 44,000 deaths recorded annually. Until today, 8 HEV genotypes are reported with only the first four genotypes infecting humans. In developing countries, HEV is transmitted via the fecal-oral route through contaminated drink...
