Kwok, Sze Hang Liu, Yuejiang Bilder, David Kim, Jung
Tumors can induce systemic disturbances in distant organs, leading to physiological changes that enhance host morbidity. In Drosophila cancer models, tumors have been known for decades to cause hypervolemic "bloating" of the abdominal cavity. Here we use allograft and transgenic tumors to show that hosts display fluid retention associated with auto...
Hutchinson, Ashlee M Appeltant, Ruth Burdon, Tom Bao, Qiuye Bargaje, Rhishikesh Bodnar, Andrea Chambers, Stuart Comizzoli, Pierre Cook, Laura Endo, Yoshinori
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Wildlife biodiversity is essential for healthy, resilient and sustainable ecosystems. For biologists, this diversity also represents a treasure trove of genetic, molecular and developmental mechanisms that deepen our understanding of the origins and rules of life. However, the rapid decline in biodiversity reported recently foreshadows a potentiall...
Hutchinson, Ashlee M Appeltant, Ruth Burdon, Tom Bao, Qiuye Bargaje, Rhishikesh Bodnar, Andrea Chambers, Stuart Comizzoli, Pierre Cook, Laura Endo, Yoshinori
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Peer reviewed: True / Publication status: Published / Funder: University of Cambridge; doi: http://dx.doi.org/10.13039/501100000735 / Wildlife biodiversity is essential for healthy, resilient and sustainable ecosystems. For biologists, this diversity also represents a treasure trove of genetic, molecular and developmental mechanisms that deepen our...
Shahbazi, Marta N.; Pasque, Vincent; 102349;
The use of stem cells to model the early human embryo promises to transform our understanding of developmental biology and human reproduction. In this review, we present our current knowledge of the first 2 weeks of human embryo development. We first focus on the distinct cell lineages of the embryo and the derivation of stem cell lines. We then di...
Siriwardena, Dylan Munger, Clara Penfold, Christopher Kohler, Timo N Weberling, Antonia Linneberg-Agerholm, Madeleine Slatery, Erin Ellermann, Anna L Bergmann, Sophie Clark, Stephen J
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Early human trophoblast development has remained elusive due to the inaccessibility of the early conceptus. Non-human primate models recapitulate many features of human development and allow access to early postimplantation stages. Here, we tracked the pre- to postimplantation transition of the trophoblast lineage in superficially implanting marmos...
Negueruela, Javier Vandenbempt, Valerie Talamantes, Stephanie Ribeiro-Costa, Francisco Nunes, Mariana Dias, André Bansal, Mayank Gurzov, Esteban N
Published in
STAR protocols
Gene editing of human pluripotent stem cells is a promising approach for developing targeted gene therapies for metabolic diseases. Here, we present a protocol for generating a CRISPR-Cas12a gene knockout of protein tyrosine phosphatases in human embryonic stem cells. We describe steps for differentiating the edited clones into pancreatic islet-lik...
Ciwinska, Marta; Messal, Hendrik A.; Hristova, Hristina R.; Lutz, Catrin; Bornes, Laura; Chalkiadakis, Theofilos; Harkes, Rolf; Langedijk, Nathalia S.M.; Hutten, Stefan J.; Menezes, Renee X.;
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Oncogenic mutations are abundant in the tissues of healthy individuals, but rarely form tumours1-3. Yet, the underlying protection mechanisms are largely unknown. To resolve these mechanisms in mouse mammary tissue, we use lineage tracing to map the fate of wild-type and Brca1-/-;Trp53-/- cells, and find that both follow a similar pattern of loss a...
Babey, Muriel E Krause, William C Chen, Kun Herber, Candice B Torok, Zsofia Nikkanen, Joni Rodriguez, Ruben Zhang, Xiao Castro-Navarro, Fernanda Wang, Yuting
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In lactating mothers, the high calcium (Ca2+) demand for milk production triggers significant bone loss1. Although oestrogen normally counteracts excessive bone resorption by promoting bone formation, this sex steroid drops precipitously during this postpartum period. Here we report that brain-derived cellular communication network factor 3 (CCN3) ...
Yang, Jing Lewis, Geoffrey Hsiang, Chin-Hui Menges, Steven Luna, Gabriel Cho, William Turovets, Nikolay Fisher, Steven Klassen, Henry
Photoreceptor degeneration is a major cause of untreatable blindness worldwide and has recently been targeted by emerging technologies, including cell- and gene-based therapies. Cell types of neural lineage have shown promise for replacing either photoreceptors or retinal pigment epithelial cells following delivery to the subretinal space, while ce...
Tsukui, Tatsuya Wolters, Paul J Sheppard, Dean
Fibroblasts are present throughout the body and function to maintain tissue homeostasis. Recent studies have identified diverse fibroblast subsets in healthy and injured tissues1,2, but the origins and functional roles of injury-induced fibroblast lineages remain unclear. Here we show that lung-specialized alveolar fibroblasts take on multiple mole...