Jin, Li-Qun Shentu, Jun-Kang Liu, Han-Lin Shao, Tian-Chen Liu, Zhi-Qiang Xue, Ya-Ping Zheng, Yu-Guo
Published in
Journal of biotechnology
Transaminases catalyze the transfer of an amino group from a donor to a keto group of an acceptor substrate and are applicable to the asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). Here, the important residue sites (C390, I22, V52, R141, Y138 and D239) of transaminase from Salmonella enterica (SeTA) were modified at the adjacency of ...
El Harrar, Till Davari, Mehdi D. Jaeger, Karl-Erich Schwaneberg, Ulrich Gohlke, Holger
Published in
Computational and Structural Biotechnology Journal
Ionic liquids (IL) and aqueous ionic liquids (aIL) are attractive (co-)solvents for green industrial processes involving biocatalysts, but often reduce enzyme activity. Experimental and computational methods are applied to predict favorable substitution sites and, most often, subsequent site-directed surface charge modifications are introduced to e...
Steiner, Paul J. Baumer, Zachary T. Whitehead, Timothy A.
Published in
Bio-protocol
Saturation mutagenesis is a fundamental enabling technology for protein engineering and epitope mapping. Nicking mutagenesis (NM) allows the user to rapidly construct libraries of all possible single mutations in a target protein sequence from plasmid DNA in a one-pot procedure. Briefly, one strand of the plasmid DNA is degraded using a nicking res...
Pan, Shiyou Yao, Tiantian Du, Liqin Wei, Yutuo
Published in
Journal of bioscience and bioengineering
Halophilic α-amylases possess optimal activity in high salt concentrations. Therefore, they can be used in many extreme conditions in industrialised production. In the present work, a halophilic α-amylase (KP) from Klebsiella pneumoniae was characterised, and it exhibited a high specific activity of 3512 U/mg under optimal conditions of 2 M NaCl at...
Rosini, Elena Pollegioni, Loredano
Published in
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
Protein engineering represents a modern approach to generate novel proteins for the different fields of biotechnology. Here, we report about an 8-day laboratory activity in which students generate enzyme variants to degrade the herbicide glyphosate. The students conduct a true research experiment in an important field (bioremediation by novel, engi...
Li, Jingmei Yu, Lei Li, Jixuan Xie, Liping Zhang, Rongqing Wang, Hongzhong
Published in
Journal of bioscience and bioengineering
Nucleoside deoxyribosyltransferase II (NDT) catalyzes the transglycosylation reaction of the 2'-deoxyribose moiety between purine and/or pyrimidine bases and has been widely used in the synthesis of nucleoside analogs. The high specificity of NDT for 2'-deoxyribose limits its applications. Because 2'C- and/or 3'C-modified nucleosides have been wide...
Tan, Chunlin Zhang, Xian Zhu, Zhijing Xu, Meijuan Yang, Taowei Osire, Tolbert Yang, Shangtian Rao, Zhiming
Published in
Microbial Cell Factories
BackgroundStyrene monooxygenase (SMO) catalyzes the first step of aromatic alkene degradation yielding the corresponding epoxides. Because of its broad spectrum of substrates, the enzyme harbors a great potential for an application in medicine and chemical industries.ResultsIn this study, we achieved higher enzymatic activity and better stability t...
Gao, Shuaihua Lu, Yingxiu Li, Yuanyuan Huang, Rong Zheng, Guojun
Published in
Applied Microbiology and Biotechnology
(−)-γ-Lactam ((−)-2-azabicyclo[2.2.1]hept-5-en-3-one) has attracted increasing attention as the chiral intermediate of carbocyclic nucleosides most of which serve as pharmaceutical agents such as anti-HIV/HBV drugs abacavir and carbovir. So far, developing in vitro (+)-γ-lactamase-mediated biotransformation has been one of the most efficient approa...
Li, Song Yang, Qian Tang, Bin Chen, Ana
Published in
Enzyme and microbial technology
Optimal pH and ideal functioning temperature for fungal α-amylase can greatly contribute to improving enzyme efficiency in maltose-forming ability. This work aimed to improve the enzymatic properties of Rhizopus oryzae α-amylase by site-saturation mutagenesis of histidine 286. The biochemical properties of selected mutant enzymes were modified to i...
Körfer, Georgette Novoa, Catalina Kern, Janina Balla, Elisabeta Grütering, Carolin Davari, Mehdi D. Martinez, Ronny Vojcic, Ljubica Schwaneberg, Ulrich
Published in
Applied Microbiology and Biotechnology
Phytases are phosphohydrolases that initiate the sequential hydrolysis of phosphate from phytate, which is the main storage form of phosphorous in numerous plant seeds, especially in cereals and grains. Phytate is indigestible for most monogastric animals, such as poultry, swine, fish, and humans; therefore, microbial phytases have been widely used...
