Wang, Minyang Shang, Yimin Liu, Xiaomeng Chen, Sanfeng
Published in
Frontiers in Microbiology
Nitrogenase in some bacteria and archaea catalyzes conversion of N2 to ammonia. To reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host is still a challenge, since synthesis of nitrogenase requires a large number of nif (nitrogen fixation) genes. Viral 2A peptide mediated “cleavage” of polyprotein is one of strategies for multigene ...
Zhang, Lijuan Liu, Xue Li, Jiawei Meng, Yonghui Zhao, Guang-Rong
Published in
Synthetic and systems biotechnology
Betanin is a member of natural pigment betacyanins family and has extensive application in the food industry as an important natural red food colorant. Its relatively inefficient production in nature however hampers access to this phytochemicals through traditional crop-based manufacturing. Microbial bioproduction therefore represents an attractive...
Ding, Yong-Wen Lu, Chuan-Zhen Zheng, Yan Ma, Han-Zhang Jin, Jin Jia, Bin Yuan, Ying-Jin
Published in
Synthetic and systems biotechnology
The accumulation of the intermediate zeaxanthin and canthaxanthin in the astaxanthin biosynthesis pathway catalyzed by β-carotene hydroxylase (crtZ) and β-carotene ketolase (crtW) decreases the content of the astaxanthin. Here, we exploited directed evolution of the fusion of crtZ and crtW for improving astaxanthin biosynthesis in Saccharomyces cer...
He, Jian-Ju Liu, Xin-Xin Li, Ying Wang, Zhe Shi, Hong-Ling Kan, Yun-Chao Yao, Lun-Guang Tang, Cun-Duo
Published in
Frontiers in Bioengineering and Biotechnology
Bordet, Fanny Romanet, Rémy Bahut, Florian Ballester, Jordi Eicher, Camille Peña, Cristina Ferreira, Vicente Gougeon, Régis Julien-Ortiz, Anne Roullier-Gall, Chloé
...
Published in
Frontiers in Microbiology
Yeast co-inoculations in winemaking are often studied in the framework of modulating the aromatic profiles of wines. Our study aimed to investigate the impact of three cocultures and corresponding pure cultures of Saccharomyces cerevisiae on the chemical composition and the sensory profile of Chardonnay wine. Coculture makes it possible to obtain c...
Santiago-Tirado, Felipe H. Hurtaux, Thomas Geddes-McAlister, Jennifer Nguyen, Duy Helms, Volkhard Doering, Tamara L. Römisch, Karin
Published in
mBio
The fungal pathogen Cryptococcus neoformans is distinguished by a cell-wall-anchored polysaccharide capsule that is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway. Protein secretion begins with polypeptide translocation across the endoplasmic reticulum (ER) membrane through a highly conserved channe...
Pouzet, Sylvain Cruz-Ramón, Jessica Le Bec, Matthias Cordier, Céline Banderas, Alvaro Barral, Simon Castaño-Cerezo, Sara Lautier, Thomas Truan, Gilles Hersen, Pascal
...
Published in
Frontiers in Bioengineering and Biotechnology
Optogenetics arises as a valuable tool to precisely control genetic circuits in microbial cell factories. Light control holds the promise of optimizing bioproduction methods and maximizing yields, but its implementation at different steps of the strain development process and at different culture scales remains challenging. In this study, we aim to...
Giacomini, Rafaela Xavier Barnes Rodrigues Cerqueira, Maristela Primel, Ednei Gilberto Garda-Buffon, Jaqueline
Published in
Ciência e Técnica Vitivinícola
This study monitored concentrations of both pesticides 2,4-dichlorophenoxyacetic acid (2,4-D) and procymidone, and mycotoxin ochratoxin A (OTA) in stages of the winemaking process. Sampling was carried out in the usual vinification process of red wine in a winery between the steps to obtain must and alcoholic fermentation. The highest transference ...
Kuribayashi, Takashi Sakurai, Toshiki Hatakeyama, Akira Joh, Toshio Kaneoke, Mitsuoki
Published in
The Journal of general and applied microbiology
In Saccharomyces cerevisiae, ethyl caprylate is produced by the esterification of caprylic acid, which is synthesized through the action of fatty acid synthase. A recent study reported a yeast mutant with a single nucleotide substitution in the alpha subunit of fatty acid synthase (FAS2) gene (F1279Y; 3836T>A) that produced large amounts of ethyl c...
Fu, Guiming Cai, Wenqin Dong, Biao Wan, Yin Pan, Fei Zheng, Fuping Chen, Yanru Deng, Mengfei Huang, Bingjing
Published in
Journal of the science of food and agriculture
Bio-augmented Daqu is used to improve the microbial community and physicochemical parameters of fermented grains, thus affecting the flavor and quality of baijiu. This study investigated the effects of bio-augmented Daqu inoculated with Aspergillus niger NCUF413.1 and Saccharomyces cerevisiae NCUF304.1 on the microbial community, aroma compounds, a...
