Freire, Claudia A Santos, Ana Carolina M Pignatari, Antonio C Silva, Rosa M Elias, Waldir P
Published in
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Extraintestinal pathogenic Escherichia coli (ExPEC) is the major cause of Gram-negative-related sepsis. Bacterial survival in the bloodstream is mediated by a variety of virulence traits, including those mediating immune system evasion. Serine protease autotransporters of Enterobacteriaceae (SPATE) constitute a superfamily of virulence factors that...
Sanchez-Villamil, Javier I. Navarro-Garcia, Fernando Castillo-Romero, Araceli Gutierrez-Gutierrez, Filiberto Tapia, Daniel Tapia-Pastrana, Gabriela
Published in
Frontiers in Cellular and Infection Microbiology
Pet and EspC are toxins secreted by enteroaggregative (EAEC) and enteropathogenic (EPEC) diarrheagenic Escherichia coli pathotypes, respectively. Both toxins are members of the Serine Protease Autotransporters of Enterobacteriaceae (SPATEs) family. Pet and EspC are important virulence factors that produce cytotoxic and enterotoxic effects on entero...
Habouria, Hajer Pokharel, Pravil Maris, Segolène Garénaux, Amélie Bessaiah, Hicham Houle, Sébastien Veyrier, Frédéric J. Guyomard-Rabenirina, Stéphanie Talarmin, Antoine Dozois, Charles M.
...
Published in
Virulence
Serine protease autotransporters of Enterobacteriaceae (SPATEs) are secreted proteins that contribute to virulence and function as proteases, toxins, adhesins, and/or immunomodulators. An extra-intestinal pathogenic E. coli (ExPEC) O1:K1 strain, QT598, isolated from a turkey, was shown to contain vat, tsh , and three uncharacterized SPATE-encoding ...
Abreu, Afonso G Abe, Cecilia M Nunes, Kamila O Moraes, Claudia T P Chavez-Dueñas, Lucia Navarro-Garcia, Fernando Barbosa, Angela S Piazza, Roxane M F Elias, Waldir P
Published in
Gut microbes
Autotransporter proteins (AT) are associated with bacterial virulence attributes. Originally identified in enteroaggregative Escherichia coli (EAEC), Shigella flexneri 2a and uropathogenic E. coli, the serine protease Pic is one of these AT. We have previously detected one atypical enteropathogenic E. coli strain (BA589) carrying the pic gene. In t...
weiss, andré kortemeier, david brockmeyer, jens
The activity of serine proteases is influenced by their substrate specificity as well as by the physicochemical conditions. Here, we present the characterization of key biochemical features of the two SPATE members EspPα and EspI from Shiga-toxin producing Escherichia coli (STEC) and enterohemorrhagic E. coli (EHEC). Both proteases show high activi...
Ruiz-Perez, Fernando Nataro, James P.
Published in
Cellular and Molecular Life Sciences
Serine proteases exist in eukaryotic and prokaryotic organisms and have emerged during evolution as the most abundant and functionally diverse group. In Gram-negative bacteria, there is a growing family of high molecular weight serine proteases secreted to the external milieu by a fascinating and widely employed bacterial secretion mechanism, known...
Weiss, André Brockmeyer, Jens
Published in
Toxins
Enterohemorrhagic E. coli (EHEC) causes severe diseases in humans worldwide. One of its virulence factors is EspP, which belongs to the serine protease autotransporters of Enterobacteriaceae (SPATE) family. In this review we recapitulate the current data on prevalence, biogenesis, structural properties and functionality. EspP has been used to inves...
Dautin, Nathalie
Published in
Toxins
Serine Protease Autotransporters of Enterobacteriaceae (SPATEs) constitute a large family of proteases secreted by Escherichia coli and Shigella. SPATEs exhibit two distinct proteolytic activities. First, a C-terminal catalytic site triggers an intra-molecular cleavage that releases the N-terminal portion of these proteins in the extracellular medi...
