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Nair, Rohini R. Haimovich, Gal Gerst, Jeffrey E.
Published in
Bio-protocol
RNA-RNA and RNA-protein interactions are involved in the regulation of gene expression. Here, we describe an updated and extended version of our RNA purification and protein identification (RaPID) protocol for the pulldown of aptamer-tagged mRNAs by affinity purification. The method takes advantage of the high affinity interaction between the MS2 R...
Wu, Yuteng Bertran, M. Teresa Rowley, James Calder, Ewen D. D. Joshi, Dhira Walport, Louise J.
Published in
Chemmedchem
Without a trace : Fluorescent amino acids ClAc−4CNW, ClAc−AzAla were incorporated into in vitro translated macrocyclic peptides by genetic code reprogramming. The minimally perturbing dyes were found to be good tools for tracking peptide penetration into cells by fluorescence imaging and flow cytometry.
Wu, Y Bertran, MT Rowley, J Calder, EDD Joshi, D Walport, LJ
The major obstacle in applying peptides to intracellular targets is their low inherent cell permeability. Standard approaches to attach a fluorophore (e.g. FITC, TAMRA) can change the physicochemical properties of the parent peptide and influence their ability to penetrate and localize in cells. We report a label-free strategy for evaluating the ce...
Naseri, Ardalan Tang, Kecong Geng, Xin Shi, Junjie Zhang, Jing Shakya, Pramesh Liu, Xiaoming Zhang, Shaojie Zhi, Degui
Published in
BMC biology
The genealogical histories of individuals within populations are of interest to studies aiming both to uncover detailed pedigree information and overall quantitative population demographic histories. However, the analysis of quantitative details of individual genealogical histories has faced challenges from incomplete available pedigree records and...
