Raddaoui, Nada Croce, Stefano Geiger, Florian Borodavka, Alexander Möckl, Leonhard Stazzoni, Samuele Viverge, Bastien Bräuchle, Christoph Frischmuth, Thomas Engelke, Hanna
...
Published in
Chembiochem
More is less ! Increasing the number of fluorophores per oligonucleotide probe allows superb sensitivity, as the fewer the number of probes needed, the lower the background noise. With just a small number of triply labeled strands, RNA‐FISH‐based detection of RNA transcripts was possible in live cells, and a virus could be detected after only 4 hou...
Stachecka, Joanna Nowacka-Woszuk, Joanna Kolodziejski, Pawel A Szczerbal, Izabela
Published in
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Proper expression of the PPARG gene, which encodes a key transcription factor of adipogenesis, is indispensable in the formation of mature adipocytes. The positioning of a gene within the nuclear space has been implicated in gene regulation. We here report on the significance of the PPARG gene's nuclear positioning for its activity during in vitro ...
Walzer, Katelyn A Kubicki, Danielle M Tang, Xiaohu Chi, Jen-Tsan Ashley
Published in
mSphere
Sexual reproduction is an obligate step in the Plasmodium falciparum life cycle, with mature gametocytes being the only form of the parasite capable of human-to-mosquito transmission. Development of male and female gametocytes takes 9 to 12 days, and although more than 300 genes are thought to be specific to gametocytes, only a few have been postul...
Chen, Christopher P Chuang, Frank Izumiya, Yoshihiro
It is well known that spatial and temporal regulation of genes is an integral part of governing proper gene expression. Consequently, it is invaluable to understand where and when transcription is taking place within nuclear space and to visualize the relationship between episomes infected within the same cell's nucleus. Here, both immunofluorescen...
Chen, Christopher P. Chuang, Frank Izumiya, Yoshihiro
Published in
Journal of Visualized Experiments : JoVE
It is well known that spatial and temporal regulation of genes is an integral part of governing proper gene expression. Consequently, it is invaluable to understand where and when transcription is taking place within nuclear space and to visualize the relationship between episomes infected within the same cell's nucleus. Here, both immunofluorescen...
Billman, MR Rueda, D Bangham, CRM
Background: The human leukaemia virus HTLV-1 expresses essential accessory genes that manipulate the expression, splicing and transport of viral mRNAs. Two of these genes, tax and hbz, also promote proliferation of the infected cell, and both genes are thought to contribute to oncogenesis in adult T-cell leukaemia/lymphoma. The regulation of HTLV...
González-Pérez, Marina Brinco, Catarina Vieira, Ricardo Rosado, Tânia Mauran, Guilhem Pereira, António Candeias, António Caldeira, Ana Teresa
Published in
Applied Physics A
The detection and analysis of metabolically active microorganisms are useful to determine those directly involved in the biodeterioration of cultural heritage (CH). Fluorescence in situ hybridization with oligonucleotide probes targeted at rRNA (RNA-FISH) has demonstrated to be a powerful tool for signaling them. However, more efforts are required ...
Koo, Dal-Hoe Zhao, Hainan Jiang, Jiming
Published in
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
Tandemly repetitive DNA sequences, also named satellite repeats, are major DNA components of heterochromatin and are often organized as long arrays in the pericentromeric, centromeric, and subtelomeric regions of eukaryotic chromosomes. An increasing amount of evidence indicates that transcripts derived from some satellite repeats play important ro...
Koo, Dal-Hoe Zhao, Hainan Jiang, Jiming
Published in
Chromosome Research
Tandemly repetitive DNA sequences, also named satellite repeats, are major DNA components of heterochromatin and are often organized as long arrays in the pericentromeric, centromeric, and subtelomeric regions of eukaryotic chromosomes. An increasing amount of evidence indicates that transcripts derived from some satellite repeats play important ro...
Zhang, Xizhe Li, Haitang Burnett, John C Rossi, John J
Published in
RNA (New York, N.Y.)
Long noncoding RNAs (lncRNAs) are known to regulate neighboring protein-coding genes by directing chromatin remodeling complexes, imprinting, and X-chromosome inactivation. In this study, we explore the function of lncRNAs in small RNA-triggered transcriptional gene activation (TGA), a process in which microRNAs (miRNAs) or small interfering RNAs (...