Tan, Ya Gan, Mailin Shen, Linyuan Li, Liang Fan, Yuan Chen, Ying Chen, Lei Niu, Lili Zhao, Ye Jiang, Anan
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Published in
International journal of molecular sciences
Gene transcripts or mRNAs and long noncoding RNAs (lncRNAs) are differentially expressed during porcine skeletal muscle development. However, only a few studies have been conducted on skeletal muscle transcriptome in pigs based on timepoints according to the growth curve for porcine. Here, we investigated gene expression in Qingyu pigs at three dif...
Miyasaka, Yuhei Komatsu, Shuichiro Abe, Takanori Kubo, Nobuteru Okano, Naoko Shibuya, Kei Shirai, Katsuyuki Kawamura, Hidemasa Saitoh, Jun-Ichi Ebara, Takeshi
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Published in
Cancers
Lung cancer is a leading cause of cancer-related deaths worldwide. Radiotherapy is an essential treatment modality for inoperable non-small cell lung cancer (NSCLC). Stereotactic body radiotherapy (SBRT) is the standard treatment for early-stage NSCLC because of its favorable local control (LC) compared to conventional radiotherapy. Carbon ion radi...
Matboli, Marwa Saad, Maha Hasanin, Amany Helmy A Saleh, Lobna Baher, Walaa Bekhet, Miram M Eissa, Sanaa
Published in
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
We intended to examine the molecular mechanism of action of isorhamnetin (IHN) to regulate the pathway of insulin signaling. Molecular analysis, immunofluorescence, and histopathological examination were used to assess the anti-hyperglycemic and insulin resistance lowering effects of IHN in streptozotocin /high fat diet-induced type 2 diabetes usin...
Sharma, Deepika Hilton, Matthew J Karner, Courtney M
Published in
Methods in molecular biology (Clifton, N.J.)
Whole mount in situ hybridization is a sensitive method used to characterize the spatial and temporal expression of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying gene expression during embryonic development. Here, we describe a procedure for digoxigenin labeled in situ hybridization on whole embryos.
Tuck, Alex C Bühler, Marc
Published in
Methods in molecular biology (Clifton, N.J.)
Some long non-coding RNA (lncRNA) genes encode a functional RNA product, whereas others act as DNA elements or via the act of transcription . We describe here a ribozyme-based approach to deplete an endogenous lncRNA in mouse embryonic stem cells, with minimal disruption of its gene. This enables the role of the lncRNA product to be tested.
Hettinger, Zachary R Confides, Amy L Vanderklish, Peter W Sidhom, Silvana Masternak, Michal M Dupont-Versteegden, Esther E
Published in
Experimental gerontology
RNA binding protein motif 3 (RBM3) is an RNA-binding and cold shock protein that protects myoblasts and promotes skeletal muscle hypertrophy by enhancing mRNA stability and translation. Muscle size is decreased during aging; however, it is typically delayed in models of extended lifespan such as the long-lived Ames Dwarf (df/df) mice and calorie re...
Miller, Jeremy A Gouwens, Nathan W Tasic, Bosiljka Collman, Forrest van Velthoven, Cindy Tj Bakken, Trygve E Hawrylycz, Michael J Zeng, Hongkui Lein, Ed S Bernard, Amy
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Published in
eLife
The advancement of single-cell RNA-sequencing technologies has led to an explosion of cell type definitions across multiple organs and organisms. While standards for data and metadata intake are arising, organization of cell types has largely been left to individual investigators, resulting in widely varying nomenclature and limited alignment betwe...
Xu, Yang Rather, Adil M Song, Shuang Fang, Jen-Chun Dupont, Robert L Kara, Ufuoma I Chang, Yun Paulson, Joel A Qin, Rongjun Bao, Xiaoping
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Published in
Cell reports. Physical science
Rapid, robust virus-detection techniques with ultrahigh sensitivity and selectivity are required for the outbreak of the pandemic coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Here, we report that the femtomolar concentrations of single-stranded ribonucleic acid (ssRNA) of SARS-CoV-2...
Tosolini, Damiano Antoniali, Giulia Dalla, Emiliano Tell, Gianluca
Published in
Biomolecular Concepts
Liquid-liquid phase separation (LLPS) is a way to concentrate biochemical reactions while excluding noninteracting components. Disordered domains of proteins, as well as interaction with RNA, favor condensation but are not mandatory for modulating this process. Recent insights about phase-separation mechanisms pointed to new fascinating models that...
Elcheva, Irina A Spiegelman, Vladimir S
Published in
Cancers
RNA molecules are a source of phenotypic diversity and an operating system that connects multiple genetic and metabolic processes in the cell. A dysregulated RNA network is a common feature of cancer. Aberrant expression of long non-coding RNA (lncRNA), micro RNA (miRNA), and circular RNA (circRNA) in tumors compared to their normal counterparts, a...
