Transcriptomic insights on fruit ripening of two varieties of Amazon açaí palm (Euterpe oleracea Mart.).
Research trends and hotspots in the immune microenvironment related to osteosarcoma and tumor cell aging: a bibliometric...
Published in Frontiers in Endocrinology
Background It is well known that cancers have a common feature that even if the environment is extremely poor in nutrients, they can still make good use of them to maintain viability as well as to produce new biomass, which is one of the reasons why tumor cells are powerfully less susceptible to senescence and death. The microenvironment has a prof...
Tho2 is critical for the recruitment of Rrp6 to chromatin in response to perturbed mRNP biogenesis
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The eukaryotic THO complex coordinates the assembly of so-called messenger RNA-ribonucleoprotein particles (mRNPs), a process that involves co-transcriptional coating nascent mRNAs with proteins. Once formed, mRNPs undergo quality control step marks them either for active transport to cytoplasm, or Rrp6/RNA exosome-mediated degradation in nucleus. ...
FUS RRM regulates poly(ADP-ribose) levels after transcriptional arrest and PARP-1 activation on DNA damage.
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Published in Cell reports
PARP-1 activation at DNA damage sites leads to the synthesis of long poly(ADP-ribose) (PAR) chains, which serve as a signal for DNA repair. Here we show that FUS, an RNA-binding protein, is specifically directed to PAR through its RNA recognition motif (RRM) to increase PAR synthesis by PARP-1 in HeLa cells after genotoxic stress. Using a structura...
The Role of Ribosomal Maturation Proteins WBSCR22 and EMG1 Methyltransferase Activity in Cancer
Internal RNA modifications are found both in coding and noncoding RNAs. In the ribosome, two highly conserved proteins catalyse the methylation of guanosine 1639 and pseudouridine 1248 residue of the 18S rRNA and they are known as WBSCR22 and EMG1. Both proteins are key factors for ribosome biogenesis and previous studies have demonstrated that in ...
Models of Hfq interactions with small non-coding RNA in Gram-negative and Gram-positive bacteria
Published in Frontiers in Cellular and Infection Microbiology
Hfq is required by many Gram-negative bacteria to chaperone the interaction between small non-coding RNA (sRNA) and mRNA to facilitate annealing. Conversely and despite the presence of Hfq in many Gram-positive bacteria, sRNAs in Gram-positive bacteria bind the mRNA target independent of Hfq. Details provided by the Hfq structures from both Gram-ne...
Paralog transcriptional differentiation in the D. melanogaster-specific gene family Sdic across populations and spermato...
How recently originated gene copies become stable genomic components remains uncertain as high sequence similarity of young duplicates precludes their functional characterization. The tandem multigene family Sdic is specific to Drosophila melanogaster and has been annotated across multiple reference-quality genome assemblies. Here we show the exist...
Precise Interference of RNA-Protein Interaction by CRISPR-Cas13-Mediated Peptide Competition.
Published in ACS synthetic biology
RNA-protein interactions are essential nodes of cellular regulatory circuits and play critical roles in normal physiology and disease. However, the precise roles of individual RNA-protein interactions remain elusive. Here we report a method for precise interference of endogenous RNA interacting with the RNA binding protein (RBP). TTP is an RBP that...
Senataxin helicase, the causal gene defect in ALS4, is a significant modifier of C9orf72 ALS G4C2 and arginine-containin...
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Identifying genetic modifiers of familial amyotrophic lateral sclerosis (ALS) may reveal targets for therapeutic modulation with potential application to sporadic ALS. GGGGCC (G4C2) repeat expansions in the C9orf72 gene underlie the most common form of familial ALS, and generate toxic arginine-containing dipeptide repeats (DPRs), which interfere wi...
Régulations de l’expression des gènes du Chloroplaste chez Chlamydomonas reinhardtii : ARN et Protéine
Le chloroplaste, siège de la photosynthèse, dériverait d’un évènement d’endosymbiose. Une cyanobactérie aurait été capturée et domestiquée pour devenir l’organite étudié à ce jour. La domestication entraina un transfert d’une majorité de son matériel génétique vers le noyau le réduisant à des gènes photosynthétiques ou essentiels et la rendant dépe...
