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BackgroundSchisandra chinensis Turcz Baill bee pollen extract SCBPE is often used as a functional food in China due to its good antioxidant property However its chemical compositions and effects on H9c2 cardiomyocytes against H2O2-induced cell injury still lacks of reports thus far This study aimed to characterize the main components of SCBPE and investigate its protective effects against H2O2-induced H9c2 cardiomyocyte injury MethodsThe main components of SCBPE were analyzed via ultraperformance liquid chromatography quadrupole time-of-flight tandem mass spectrometry UPLC QTOF MS MS The three main nucleosides in SCBPE were quantitatively analyzed via ultraperformance liquid chromatography diode array detection Furthermore the potential mechanism by which SCBPE exerts protective effects against H2O2-induced H9c2 cardiomyocyte injury was explored for the first time via cell survival rate measurements cell morphological observation myocardial superoxide dismutase SOD activity and malondialdehyde MDA and glutathione GSH level determination flow cytometry and quantitative polymerase chain reaction ResultsTwo carbohydrates three nucleosides and nine quinic acid nitrogen-containing derivatives in SCBPE were identified or tentatively characterized via UPLC QTOF MS MS The nine quinic acid nitrogen-containing derivatives were first reported in bee pollen The contents of uridine guanosine and adenosine were 2 4945 0 0185 0 1896 0 0049 and 1 8418 0 0157 g mg respectively Results of in vitro experiments showed that cell survival rate myocardial SOD activity and GSH level significantly increased and myocardial MDA level significantly decreased in SCBPE groups compared with those in H2O2 group Cell morphology in SCBPE groups also markedly improved compared with that in H2O2 group Results indicated that SCBPE protected H9c2 cardiomyocytes from H2O2-induced apoptosis by downregulating the mRNA expressions of Bax cytochrome C and caspase-3 and upregulating the Bcl-2 mRNA expression ConclusionsThis study is the first to report that SCBPE could protect against oxidative stress injury and apoptosis in H2O2-injured H9c2 cells Results indicated that the nucleosides and quinic acid nitrogen-containing derivatives could be the main substances that exert protective effects against H2O2-induced H9c2 cardiomyocyte injury

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Schisandra chinensis bee pollen’s chemical profiles and protective effect against H2O2-induced apoptosis in H9c2 cardiom...

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with Quinic acid nitrogen-containing derivatives as keyword