Rajoria, Sakshi Nissa, Mehar Un Suvarna, Kruthi Khatri, Harsh Srivastava, Sanjeeva
Published in
Data in brief
Elucidation of molecular markers related to the mounted immune response is crucial for understanding the disease pathogenesis. In this article, we present the mass-spectrometry-based metabolomic and proteomic data of blood plasma of COVID-19 patients collected at two-time points, which showed a transition from non-severe to severe conditions during...
Stangler, Luke A Nicolai, Evan N Mivalt, Filip Chang, Su-Youne Kim, Inyong Kouzani, Abbas Z Bennet, Kevin Berk, Michael Uthamaraj, Susheil Burns, Terry C
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Published in
Journal of Neural Engineering
Objective. To modify off-the-shelf components to build a device for collecting electroencephalography (EEG) from macroelectrodes surrounded by large fluid access ports sampled by an integrated microperfusion system in order to establish a method for sampling brain interstitial fluid (ISF) at the site of stimulation or seizure activity with no bias ...
Strefeler, Abigail Jan, Maxime Quadroni, Manfredo Teav, Tony Rosenberg, Nadia Chatton, Jean-Yves Guex, Nicolas Gallart-Ayala, Hector Ivanisevic, Julijana
Published in
Alzheimer's research & therapy
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that is characterized by altered cellular metabolism in the brain. Several of these alterations have been found to be exacerbated in females, known to be disproportionately affected by AD. We aimed to unravel metabolic alterations in AD at the metabolic pathway level and evaluate ...
Zhang, Fan Cleaver, Sophie Gardner, Matthew Briones, Andrea Soloviev, Mikhail
Published in
Methods in molecular biology (Clifton, N.J.)
This chapter describes the principles for selection of antigenic peptides for the development of anti-peptide antibodies suitable for microarray-based multiplex affinity assays and optional mass spectrometry detection. The methods described here are mostly applicable to small- and medium-scale multiplex affinity assay and microarrays. Although the ...
Dunphy, Katie Dowling, Paul
Published in
Methods in molecular biology (Clifton, N.J.)
Cancer of blood or bone marrow-derived cells dysregulates normal hematopoiesis and accounts for over 6% of all cancer cases annually. Proteomic analyses of blood cancers have improved our understanding of disease mechanisms and identified numerous proteins of clinical relevance. For many years, gel-based proteomic studies have aided in the discover...
Dani, Diksha Dencher, Norbert A
Published in
Methods in molecular biology (Clifton, N.J.)
Protein-protein interactions and multiprotein assemblies of water-soluble and membrane proteins are inherent features of the proteome, which also impart functional heterogeneity. One needs to consider this aspect while studying changes in abundance and activities of proteins in response to any physiological stimulus. Abundance changes in the compon...
Kamata, Shotaro Ishii, Isao
Published in
Methods in molecular biology (Clifton, N.J.)
Several years have passed since LC (liquid chromatography)-MS (mass spectrometry) became the mainstream for proteomic analysis; however, conventional fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) continues to be an important technology that enables rapid and direct visualization of hundreds to thousands of proteins and their...
O'Sullivan, Eleanor M Dowling, Paul Swandulla, Dieter Ohlendieck, Kay
Published in
Methods in molecular biology (Clifton, N.J.)
Many biomedically relevant biomarkers are proteins with characteristic biochemical properties and a relatively restricted subcellular distribution. The comparative and mass spectrometry-based proteomic analysis of body fluids can be particularly instrumental for the targeted identification of novel protein biomarkers with pathological relevance. In...
Steffen, Brian T Tang, Weihong Lutsey, Pamela L Demmer, Ryan T Selvin, Elizabeth Matsushita, Kunihiro Morrison, Alanna C Guan, Weihua Rooney, Mary R Norby, Faye L
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Published in
Diabetologia
Genetic predisposition to type 2 diabetes is well-established, and genetic risk scores (GRS) have been developed that capture heritable liabilities for type 2 diabetes phenotypes. However, the proteins through which these genetic variants influence risk have not been thoroughly investigated. This study aimed to identify proteins and pathways throug...
Ohlendieck, Kay
Published in
Methods in molecular biology (Clifton, N.J.)
The skeletal muscle proteome consists of a large number of diverse protein species with a broad and dynamic concentration range. Since mature skeletal muscles are characterized by a distinctive combination of contractile cells with differing physiological and biochemical properties, it is essential to determine specific differences in the protein c...
