Boroumand, Mozhgan Iavarone, Federica Manconi, Barbara Pieroni, Luisa Greco, Viviana Vento, Giovanni Tirone, Chiara Desiderio, Claudia Fiorita, Antonella Faa, Gavino
...
Published in
Talanta
To have information on the proteolytic activity of convertases and exo-peptidases on human salivary proteins, this study investigated the relative amounts of the truncated proteoforms in the saliva of preterm newborns and compared them with the relative amounts measured in saliva of at-term newborns, of babies (0-10 years old) and of adults. Result...
Rausch, Valentina Sala, Valentina Penna, Fabio Porporato, Paolo Ettore Ghigo, Alessandra
Published in
Oncogenesis
Cachexia is a severe complication of cancer that adversely affects the course of the disease, with currently no effective treatments. It is characterized by a progressive atrophy of skeletal muscle and adipose tissue, resulting in weight loss, a reduced quality of life, and a shortened life expectancy. Although the cachectic condition primarily aff...
Prabudiansyah, I. (author) van der Valk, R.A. (author) Aubin-Tam, M.E. (author)
FtsH is a membrane-bound protease that plays a crucial role in proteolytic regulation of many cellular functions. It is universally conserved in bacteria and responsible for the degradation of misfolded or misassembled proteins. A recent study has determined the structure of bacterial FtsH in detergent micelles. To properly study the function of Ft...
Hofsetz, Eduard Huesgen, Pitter F Trifunovic, Aleksandra
Published in
Methods in molecular biology (Clifton, N.J.)
Mitochondrial proteases constitute a fundamental part of the organellar protein quality control system to ensure the timely removal of damaged or obsolete proteins. The analysis of proteases is often limited to the identification of bona fide substrates that are degraded in the presence and become more abundant in the absence of the respective prot...
Zhao, Di Li, Hao Huang, Mingxuan Wang, Taolin Hu, Yue Wang, Liping Xu, Dening Mao, Shengyong Li, Chunbao Zhou, Guanghong
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Published in
Food chemistry
Enzymatic tenderization is extensively applied in the meat industry, whereas its influence on meat flavor has seldom been evaluated. Proteinase K, papain, bromelain and Flavourzyme® were used to treat beef muscle, and the changes in volatile compounds and odors were subsequently analyzed. Proteolysis by proteinase K was found to elevate the average...
Yesbolatova, Aisha Saito, Yuichiro Kitamoto, Naomi Makino-Itou, Hatsune Ajima, Rieko Nakano, Risako Nakaoka, Hirofumi Fukui, Kosuke Gamo, Kanae Tominari, Yusuke
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Published in
Nature Communications
Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.
Wei, Jingjing Huang, Jiao Kuang, Yunchun Li, Yongkai Zhong, Daiqin Song, Jinlin
Published in
Archives of oral biology
We aimed to explore the effects of metformin on oral squamous cell carcinoma (OSCC) cell proliferation and the associated molecular mechanisms. We established an OSCC model in SCC15 cells overexpressing nerve growth factor receptor (NGFR) or the N-terminal region (aa 1-250; NGFR-N), and assessed cell proliferation by CCK-8 assay, colony formation a...
Strack, Philip R. Brodie, Erica J. Zhan, Hanmiao Schuenemann, Verena J. Valente, Liz J. Saiyed, Tamanna Lowth, Bradley R. Angley, Lauren M. Perugini, Matthew A. Zeth, Kornelius
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Published in
Communications Biology
Strack et al find that Polymerase δ interacting protein 38 (PDIP38) is targeted to the mitochondrial matrix where it colocalises with the mitochondrial AAA + protein CLPXP. PDIP38 modulates the specificity of CLPXP in vitro and alters the stability of CLPX in vitro and in cells. The PDIP38 structure leads the authors to speculate that PDIP38 is a C...
Eckhard, Ulrich Blöchl, Constantin Jenkins, Benjamin G. L. Mansfield, Michael J. Huber, Christian G. Doxey, Andrew C. Brandstetter, Hans
Published in
Scientific Reports
Flagellins are the protein components of bacterial flagella and assemble in up to 20,000 copies to form extracellular flagellar filaments. An unusual family of flagellins was recently discovered that contains a unique metalloprotease domain within its surface-exposed hypervariable region. To date, these proteolytic flagellins (also termed flagellin...
Li, Yan Sun, Xiaohan Gao, Dongqing Ding, Yan Liu, Jinxiao Chen, Jiong Luo, Jun Zhang, Junzheng Liu, Qingxin Zhou, Zizhang
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Published in
Cell Death & Differentiation
Hedgehog (Hh) pathway plays multiple roles in many physiological processes and its dysregulation leads to congenital disorders and cancers. Hh regulates the cellular localization of Smoothened (Smo) and the stability of Cubitus interruptus (Ci) to fine-tune the signal outputs. However, the underlying mechanisms are still unclear. Here, we show that...
