We report the photolysis of biomolecules in a Fourier-transform ion cyclotron resonance mass spectrometer by intense near-infrared femtosecond laser pulses. Photo-fragmentation was accompanied by photo-ionization and created a large number and variety of charged fragments, which could be identified with high confidence due to the outstanding resolv...
We report the photolysis of biomolecules in a Fourier-transform ion cyclotron resonance mass spectrometer by intense near-infrared femtosecond laser pulses. Photo-fragmentation was accompanied by photo-ionization and created a large number and variety of charged fragments, which could be identified with high confidence due to the outstanding resolv...
Published in AAPS PharmSciTech
Protein biologics are prone to conformational changes during formulation development. Limited methods are available for conformational analysis of proteins in solid state and in the presences of formulation excipients. The aim of this study was to investigate the secondary structures of proteins encased in solid lipid matrices as a novel indicator ...
The methylotrophic yeast Komagataella (Pichia) pastoris has become one of the most utilized cell factories for the production of recombinant proteins over the last three decades. This success story is linked to its specific physiological traits, i.e., the ability to grow at high cell density in inexpensive culture medium and to secrete proteins at ...
Key modifying roles of microbial transglutaminase (MTGase) in the development of innovative probiotic and non-probiotic yogurts with improved functional and quality characteristics have been comprehensively reviewed. MTGase crosslinking reactions with milk proteins stabilize the three-dimensional structure of yogurt. Yogurts treated with MTGase sho...
The nuclear magnetic relaxation dispersion (NMRD) technique consists of measurement of the magnetic-field dependence of the longitudinal nuclear-spin-lattice relaxation rate 1/T 1. Usually, the acquisition of the NMRD profiles is made using a fast field cycling (FFC) NMR technique that varies the magnetic field and explores a very large range of La...
The nuclear magnetic relaxation dispersion (NMRD) technique consists of measurement of the magnetic-field dependence of the longitudinal nuclear-spin-lattice relaxation rate 1/T 1. Usually, the acquisition of the NMRD profiles is made using a fast field cycling (FFC) NMR technique that varies the magnetic field and explores a very large range of La...
Yeast is very well known eukaryotic organism for its remarkable biodiversity and extensive industrial applications. Saccharomyces cerevisiae is one of the most widely used microorganisms in biotechnology with successful applications in the biochemical production. Biological conversion with the focus on the different utilization of renewable feedsto...
Ionic liquids (ILs) have attracted tremendous interest in the last decades. This is due to their high chemical variability and their interesting and useful chemical, physical, and biological properties. The current chapter provides an overview on the use and the potential of ILs for biopolymer dissolution and processing, for chemical modification o...
