Les thioltransférases à domaine rhodanèse sont des enzymes ubiquitaires qui catalysent une réaction de transfert de soufre d’un substrat donneur vers un accepteur, via un mécanisme à deux étapes impliquant la formation d’un intermédiaire persulfure sur la cystéine catalytique. Alors que ces enzymes sont classées en deux groupes distincts, les thios...
Published in Informatics in Medicine Unlocked
Coronavirus disease 2019 (COVID-19)-driven global pandemic triggered innumerable health complications, imposing great challenges in managing other respiratory diseases like asthma. Furthermore, increases in the underlying inflammation involved in the fatality of COVID-19 have been linked with lack of vitamin D. In this research work, we intend to i...
The modeling of protein assemblies at the atomic level remains a central issue in structuralbiology, as protein interactions play a key role in numerous cellular processes. This problem istraditionally addressed using docking tools, where the quality of the models is based on theirsimilarity to a single reference experimental structure. However, us...
BRCA2 is an oncoprotein frequently mutated in hereditary breast cancers. To improve the diagnosis of these cancers, several molecular studies have identified BRCA2 key positions and characterized mutations in these regions causing a BRCA2 loss of function. However, these studies mainly focused on the C-terminal globular domain of BRCA2. Here, we ch...
Published in Cytokine & growth factor reviews
Fibroblast growth factors 1 and 2 (FGF1 and FGF2) are mainly considered as ligands of surface receptors through which they regulate a broad spectrum of biological processes. They are secreted in non-canonical way and, unlike other growth factors, they are able to translocate from the endosome to the cell interior. These unique features, as well as ...
Published in Angewandte Chemie (International Ed. in English)
A modified implementation of DNA‐PAINT microscopy is used to detect spatial proximity of biomolecules with super‐resolution capabilities. The new technique, called Proximity‐PAINT, features a precisely tunable detection range, high sensitivity and low false‐positive rates. The implementation can be applied to visualize cellular protein‐protein inte...
Increasing evidence support the idea that proteins involved in RNA metabolism such as RNA binding proteins (RBPs) and RNA helicases are directly implicated in the DNA damage response (DDR). This activity is generally achieved through their interaction with DNA repair factors.BRCA2 is a tumor suppressor protein that plays an important role in the re...
Published in Bio-protocol
The mechanisms of virulence and immunity are often governed by molecular interactions between pathogens and host proteins. The study of these interactions has major implications on understanding virulence activities, and how the host immune system recognizes the presence of pathogens to initiate an immune response. Frequently, the association betwe...
Published in Molecular & cellular proteomics : MCP
We present a technological advancement for the estimation of the affinities of Protein-Protein Interactions (PPIs) in living cells. A novel set of vectors is introduced that enables a quantitative yeast two-hybrid system based on fluorescent fusion proteins. The vectors allow simultaneous quantification of the reaction partners (Bait and Prey) and ...
Published in Methods in molecular biology (Clifton, N.J.)
Few approaches are currently available that allow the detection of protein-protein interactions (PPIs) in super-resolution, and the observation of the assembly of protein complexes in living cells has been particularly challenging. We developed reconstituted fluorescence-based stochastic optical fluctuation imaging (refSOFI), which is based on bimo...
