PurposeTo compare the diagnostic utility of metagenomic deep sequencing (MDS) to cytology, flow cytometry and gene rearrangement by PCR in ocular samples of patients with suspected vitreoretinal lymphoma (VRL).MethodsPatients with suspected VRL underwent ocular sampling of one or both eyes at the Emory Eye Center from September 2017 to June 2022. O...
Published in Basic and clinical andrology
RéSUMé: CONTEXTE: Malgré de nombreux efforts pour démontrer la présence du SRAS-CoV-2 dans le sperme des hommes concernés, il n’existe aucune preuve claire. Nous avons mené une étude prospective multicentrique chez des patients adultes ayant un diagnostic confirmé de SRAS-CoV-2, comprenant des patients atteints d’une infection active (groupe actif)...
Fixation and demineralization protocols for bone marrow (BM) across diagnostic laboratories are not standardized. How different protocols affect histomorphology and DNA amplification is incompletely understood. In this study, 2 fixatives and 3 demineralization methods were tested on canine BM samples. Twenty replicate sternal samples obtained withi...
Peer reviewed: True / Publication status: Published / AIM: To validate the diagnostic performance of a custom 96-micro-organism TaqMan PCR card (iCAM) for microbial keratitis (MK) from a single corneal epithelial sample. METHODS: Patients over the age of 18 referred to Cambridge University Hospital with MK were recruited in this single-site prospec...
OBJECTIVE: To document the course of neonatal and short-term outcomes in pregnancies after first trimester CMV (cytomegalovirus) seroconversion and negative amniotic fluid (AF) CMV PCR. METHODS: We included 375 patients with a first-trimester CMV seroconversion and amniocentesis at ≥21 weeks. Termination of pregnancy (TOP) was offered in case anten...
Publication status: Published / Funder: Medical Research Council; doi: http://dx.doi.org/10.13039/501100000265 / Low availability of routine nucleic acid amplification testing (NAAT) during infection outbreaks, especially in less resourced environments, was highlighted by the Covid pandemic. One of the barriers lies with the supply chain and cost o...
Adult female and male Gongylonema nematodes were found in the oesophagus of a free-living roe deer (Capreolus capreolus) in Slovenia during passive health surveillance of wildlife. The genus Gongylonema was determined by light microscopy based on the genus-specific cuticular bosses in the anterior part of the parasite. Molecular methods were used t...
Digital PCR (dPCR) holds immense potential for precisely detecting nucleic acid markers essential for personalized medicine. However, its broader application is hindered by high consumable costs, complex procedures, and restricted multiplexing capabilities. To address these challenges, an all-in-one dPCR system is introduced that eliminates the nee...
Published in Revista Mvz Córdoba
Objective: the objective of the present work was the detection of Leishmania sp. in Rattus rattus tail base skin through polymerase chain reaction (PCR) techniques. Material and Method: We analyzed 45 Rattus rattus skin samples from the urban area of the city of Corrientes in Argentina. The Leishmania spp detection was performed by nested PCR techn...
Introduction: This research work is designed to identify biomolecules from Earthworm Coelomic Fluid (ECF) of Eudrilus Eugeniae (EE) that can inhibit cancer cellproliferation [...]
