Ansari, Mohammad Azam Taha, Muhammad Uddin, Nizam Rahim, Fazal Jamal, Qazi Mohammad Sajid Alomary, Mohammad N. Alshabrmi, Fahad M. Almatroudi, Ahmad Atwah, Banan Alhindi, Zain
...
Published in
Journal of Saudi Chemical Society
In the present study, Indole-based-oxadiazole (1A-17A) compounds were successfully synthesized. The structures of all synthesized compounds were fully characterized by different sophisticated spectroscopic techniques such 1H NMR, 13C NMR, and HREI-MS. Further, the synthesized compounds were explored to investigate their broad-spectrum antibacterial...
Giacomucci, Sean Alvarez, Laura Rodrigues, Christopher D.A. Cava, Felipe Paradis-Bleau, Catherine
While many mechanisms governing bacterial envelope homeostasis have been identified, others remain poorly understood. To decipher these processes, we previously developed an assay in the Gram-negative model Escherichia coli to identify genes involved in maintenance of envelope integrity. One such gene was ElyC, which was shown to be required for en...
Wang, Lanqi Cheng, Binbin Ju, Qiang Sun, Bryan K.
Published in
Journal of Innate Immunity
Bacterial peptidoglycan (PGN) stimulates toll-like receptor 2 (TLR2) on the surface of keratinocytes (KCs), triggering signaling pathways that promote an innate immune response. However, excessive TLR2 activation can lead to inappropriate inflammation, which contributes to skin conditions such as rosacea. To better treat these conditions, there is ...
Ismalaj, E; De Borggraeve, W; 2838;
Penicillins are a subclass of the beta-lactam antibiotics displaying a marked bactericidal activity because of their bacterial cell wall synthesis inhibition. Since their discovery, they still remain among the most used antibiotics in clinics. However, bacterial resistance development urges continuous synthetic efforts to expand the arsenal of anti...
Dai, Yunfei Pinedo, Victor Tang, Amy Y. Cava, Felipe Geisinger, Edward
The hospital-acquired pathogen Acinetobacter baumannii possesses a complex cell envelope that is key to its multidrug resistance and virulence. The bacterium, however, lacks many canonical enzymes that build the envelope in model organisms. Instead, A. baumannii contains a number of poorly annotated proteins that may allow alternative mechanisms of...
Sassine, Jad Pazos, Manuel Breukink, Eefjan Vollmer, Waldemar
Published in
Cell surface (Amsterdam, Netherlands)
Bacteria encase their cytoplasmic membrane with peptidoglycan (PG) to maintain the shape of the cell and protect it from bursting. The enlargement of the PG layer is facilitated by the coordinated activities of PG synthesising and -cleaving enzymes. In Escherichia coli, the cytoplasmic membrane-bound lytic transglycosylase MltG associates with PG s...
Simpson, Brent W. Nieckarz, Marta Pinedo, Victor McLean, Amanda B. Cava, Felipe Trent, M. Stephen
Gram-negative bacteria resist external stresses due to cell envelope rigidity, which is provided by two membranes and a peptidoglycan layer. The outer membrane (OM) surface contains lipopolysaccharide (LPS; contains O-antigen) or lipooligosaccharide (LOS). LPS/LOS are essential in most Gram-negative bacteria and may contribute to cellular rigidity....
Kim, Chan-Jin Si, Zhangyong Reghu, Sheethal Guo, Zhong Zhang, Kaixi Li, Jianghua Chan-Park, Mary B
Published in
Drug delivery and translational research
We report the first demonstration of the efficient bacteria targeting properties of DNA-based polymeric micelles with high-density DNA corona. Nanoscale polymer micelles derived from DNA-b-polystyrene (DNA-b-PS) efficiently selected most tested Gram-positive strains over Gram-negative strains; single-strand DNAs were 20-fold less selective. We demo...
Alabdali, Yasir Adil Jabbar Oatley, Peter Kirk, Joseph A Fagan, Robert P
Published in
Anaerobe
Sporulation is a complex cell differentiation programme shared by many members of the Firmicutes, the end result of which is a highly resistant, metabolically inert spore that can survive harsh environmental insults. Clostridioides difficile spores are essential for transmission of disease and are also required for recurrent infection. However, the...
Nguyen, Van Spinelli, Silvia Cascales, Éric Roussel, Alain Cambillau, Christian Leone, Philippe
The type VI secretion system (T6SS) is a widespread mechanism of protein delivery into target cells, present in more than a quarter of all sequenced Gram-negative bacteria. The T6SS constitutes an important virulence factor, as it is responsible for targeting effectors in both prokaryotic and eukaryotic cells. The T6SS comprises a tail structure te...
