Carrasco-Navarro, Ulises Vera-Estrella, Rosario Barkla, Bronwyn J. Zúñiga-León, Eduardo Reyes-Vivas, Horacio Fernández, Francisco J. Fierro, Francisco
Published in
Microbial Cell Factories
BackgroundThe heterotrimeric Gα protein Pga1-mediated signaling pathway regulates the entire developmental program in Penicillium chrysogenum, from spore germination to the formation of conidia. In addition it participates in the regulation of penicillin biosynthesis. We aimed to advance the understanding of this key signaling pathway using a prote...
Martín, Juan F.
Published in
Journal of Industrial Microbiology & Biotechnology
Penicillium chrysogenum is an excellent model fungus to study the molecular mechanisms of control of expression of secondary metabolite genes. A key global regulator of the biosynthesis of secondary metabolites is the LaeA protein that interacts with other components of the velvet complex (VelA, VelB, VelC, VosA). These components interact with Lae...
Xu, Xinxin Yang, Jing An, Yang Pan, Yuanyuan Liu, Gang
Published in
Biotechnology Letters
Rab GTPase is required for vesicle–vacuolar fusion during the vacuolar biogenesis in fungi. Rab GTPase-encoding gene, pcvA, was cloned from Penicillium chrysogenum: it contained five introns and its predicted protein contained the conserved Rab GTPase domain involved in GTP-binding and hydrolysis. Over-expression of pcvA significantly stimulated th...
Moreno-Vargas, Liliana Correa-Basurto, Jose Maroun, Rachid C. Fernández, Francisco J.
Published in
Journal of Molecular Modeling
In the last step of penicillin biosynthesis, acyl-CoA:isopenicillin N acyltransferase (IAT) (E.C. 2.3.1.164) catalyzes the conversion of isopenicillin N (IPN) to penicillin G. IAT substitutes the α-aminoadipic acid side chain of IPN by a phenylacetic acid phenolate group (from phenylacetyl-CoA). Having a three-dimensional (3D) structure of IAT help...
García-Estrada, Carlos Vaca, Inmaculada Lamas-Maceiras, Mónica Martín, Juan Francisco
Published in
Applied Microbiology and Biotechnology
Penicillium chrysogenum npe10 (Δpen; lacking the 56.8-kbp amplified region containing the penicillin gene cluster), complemented with one, two, or three penicillin biosynthetic genes, was used for in vivo studies on transport of benzylpenicillin intermediates. 6-Aminopenicillanic acid (6-APA) was taken up efficiently by P. chrysogenum npe10 unlike ...
Spröte, Petra Brakhage, Axel A.
Published in
Archives of Microbiology
The biosynthesis of the β-lactam antibiotic penicillin in Aspergillus nidulans is catalysed by three enzymes that are encoded by the genes acvA, ipnA and aatA. Several studies have indicated that these genes are controlled by a complex regulatory network, including a variety of cis-acting DNA elements and regulatory factors. Until now, however, rel...
Naranjo, Leopoldo Lamas-Maceiras, Mònica Ullán, Ricardo V. Campoy, Sonia Teijeira, Fernando Casqueiro, Javier Martín, Juan F.
Published in
Molecular Genetics and Genomics
The Penicillium chrysogenumoat1 gene, which encodes a class III ω-aminotransferase, was cloned and characterized. This enzyme converts lysine into 2-aminoadipic semialdehyde, and plays an important role in the biosynthesis of 2-aminoadipic acid, a precursor of penicillin and other β-lactam antibiotics. The enzyme is related to ornithine-5-aminotran...
Valmaseda, E. M. Martín de. Campoy, S. Naranjo, L. Casqueiro, J. Martín, J. F.
Published in
Molecular Genetics and Genomics
The biosynthesis and catabolism of lysine in Penicillium chrysogenum is of great interest because these pathways provide 2-aminoadipic acid, a precursor of the tripeptide δ-L-2-aminoadipyl-L-cysteinyl-D-valine that is an intermediate in penicillin biosynthesis. In vivo conversion of labelled L-lysine into two different intermediates was demonstrate...
Howard-Jones, Annaleise R. Rutledge, Peter J. Clifton, Ian J. Adlington, Robert M. Baldwin, Jack E.
Published in
Biochemical and Biophysical Research Communications
