Silva, Aniel de Sarom Negrão Silva, Clayton Pereira Barata, Rafael Ribeiro da Silva, Pedro Victor Reis Monteiro, Patrícia Danin Jordão Lamarão, Letícia Burbano, Rommel Mário Rodríguez Nunes, Márcio Roberto Teixeira de Lima, Patrícia Danielle Lima
Published in
Virology Journal
BackgroundHuman pegivirus (HPgV)—formerly known as GBV-C—is a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This st...
Arhab, Yani Bulakhov, Alexander G. Pestova, Tatyana V. Hellen, Christopher U.T.
Published in
Viruses
Members of Picornaviridae and of the Hepacivirus , Pegivirus and Pestivirus genera of Flaviviridae all contain an internal ribosomal entry site (IRES) in the 5′-untranslated region (5′UTR) of their genomes. Each class of IRES has a conserved structure and promotes 5′-end-independent initiation of translation by a different mechanism. Picornavirus 5...
de Albuquerque, Pedro Pereira Lira Furta... Santos, Lucianna H S Antunes, Deborah Caffarena, Ernesto Raul Figueiredo, Andreza Soriano
Published in
Virus research
Infections produced by hepaciviruses have been associated with liver disease in horses. Currently, at least three viruses belonging to the Flaviviridae family are capable of producing a chronic infection in equines: non-primate hepacivirus (NPHV), Theiler's disease-associated virus (TDAV), and equine pegivirus (EPgV). The RNA-dependent RNA polymera...
Forberg, Kenn Rodgers, Mary A Dawson, George J Sauleda, Silvia Olivo, Ana Vallari, Ana Bes, Marta Piron, Maria Cloherty, Gavin A Berg, Michael G
...
Published in
Virology
We applied an NGS based target capture approach to amplify HPgV-2 sequences from metagenomic libraries and enable full genome characterization. Despite expanded geographical sampling, sequence variability remains low, with diversity concentrated in approximately 3.3% of all amino acids. Serial samples from one HPgV-2 positive individual co-infected...
Paim, W P Weber, M N Cibulski, S P da Silva, M S Puhl, D E Budaszewski, R F Varela, A P M Mayer, F Q Canal, C W
Published in
Biologicals : journal of the International Association of Biological Standardization
Horses are often used as blood donors for commercial horse serum (HS) production and to manufacture biologicals. HS is an alternative for fetal bovine serum (FBS) used as a supplement for cell culture and vaccine production. Furthermore, HS is also frequently obtained in order to produce antisera toxins and pathogens. The advent of high-throughput ...
Chen, Fangzhou Knutson, Todd P Braun, Eli Jiang, Yin Rossow, Stephanie Marthaler, Douglas G
Published in
Transboundary and emerging diseases
Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi-quantitative RT-PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota-1/2016 had a 95.5%-96.3% nucleotide identity ...
Vitrenko, Yakov Kostenko, Iryna Kulebyakina, Kateryna Sorochynska, Khrystyna
Published in
Virology Journal
BackgroundHuman pegivirus-1 (HPgV-1) is a member of the Flaviviridae family whose genomic organization and mode of cellular entry is similar to that of hepatitis C virus (HCV). The E2 glycoprotein of HPgV-1 is the principle mediator in the virus-cell interaction and as such harbors most of HPgV-1’s antigenic determinants. HPgV-1 persists in blood c...
Frankel, Matthew Forberg, Kenn Coller, Kelly E Berg, Michael G Hackett, John Jr Cloherty, Gavin Dawson, George J
Published in
Journal of virological methods
Human Pegivirus 2 (HPgV-2) was recently identified in the bloodstream of HCV-infected and multiply transfused individuals. Initial reports show HPgV-2 circulates at a low prevalence in HCV co-infected individuals, necessitating testing of large cohorts of samples to identify infected persons. The identification of additional HPgV-2 cases was facili...
Frankel, Matthew Forberg, Kenn Coller, Kelly E Berg, Michael G Hackett, John Jr Cloherty, Gavin Dawson, George J
Published in
Journal of virological methods
Human Pegivirus 2 (HPgV-2) was recently identified in the bloodstream of HCV-infected and multiply transfused individuals. Initial reports show HPgV-2 circulates at a low prevalence in HCV co-infected individuals, necessitating testing of large cohorts of samples to identify infected persons. The identification of additional HPgV-2 cases was facili...
Bonsall, David Gregory, William F Ip, Camilla L C Donfield, Sharyne Iles, James Ansari, M Azim Piazza, Paolo Trebes, Amy Brown, Anthony Frater, John
...
Published in
Emerging infectious diseases
Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 per...