The droplet-vitrification method was adapted to Pelargonium apices by optimizing the duration of the loading solution (LS) as well as the plant vitrification solution 2 (PVS2). The excised apices were dehydrated in two steps (20 min in LS and 15 min in PVS2) and then immersed directly in liquid nitrogen (LN). After thawing and unloading in the reco...
The droplet-vitrification method was adapted to Pelargonium apices by optimizing the duration of the loading solution (LS) as well as the plant vitrification solution 2 (PVS2). The excised apices were dehydrated in two steps (20 min in LS and 15 min in PVS2) and then immersed directly in liquid nitrogen (LN). After thawing and unloading in the reco...
In order to guarantee safe, long-term conservation of the National Institute of Horticulture Pelargonium collection, apex cryopreservation studies have been undertaken since 1999. The first results were obtained on apices of P. x peltatum 'Balcon Lilas', using an encapsulation-dehydration process. Various steps of this process (preculture, dehydrat...
Une étude de la résistance à la sécheresse des pélargoniums a été réalisée. Les P.x peltatum sont les plus tolérants alors que les génotypes aromatiques et P.x domesticum sont les plus sensibles. Les P.x hortorum ont une sensibilité variable. L'accumulation de sucres (saccharose et autres de poids mléculaire plus élevé) est caractéristique des géno...