Phenolic compounds are an interesting class of natural products because of their proposed contribution to health benefits of foods and beverages and as a bio-source of organic (aromatic) building blocks. Phenolic extracts from natural products are often highly complex and contain compounds covering a broad range in molecular properties. While many ...
On-line comprehensive two-dimensional liquid chromatography is a powerful technique for the separation of highly complex samples. Due to the addition of the second dimension of separation, impressive peak capacities can be obtained within a reasonable analysis time compared to one-dimensional liquid chromatography. In online comprehensive two-dimen...
A systematic evaluation of four different ICP sample introduction systems to be used in the context of metabolite profiling of chlorine-containing pharmaceuticals via HPLC-ICP-MS was carried out using diclofenac and its major metabolite, 4'-hydroxy-diclofenac, as model compounds. The strict requirements for GMP validation of chromatographic methods...
Comprehensive two-dimensional liquid chromatography (LC X LC) allows for substantial gains in theoretical peak capacity in the field of liquid chromatography. However, in practice, theoretical performance is rarely achieved due to a combination of undersampling, orthogonality, and refocusing issues prevalent in many LC X LC applications. This is in...
Mathematical kinetic plot expressions have been established for the correct extrapolation of the kinetic performance measured in a thin-film capillary GC column with fixed length into the performance that can be expected in a longer column used at the same outlet velocity but at either the maximal inlet pressure or at the optimal inlet pressure, i....
Comprehensive two-dimensional liquid chromatography (LC x LC) has significantly improved the separation power of LC for complex samples. Our review opens with theoretical considerations about online LC x LC in order to explain why ultra-high-pressure LC (UHPLC) and more specifically, hightemperature (HT)-UHPLC are very convenient in the second dime...
In this second paper of a two-part series, on-line RPLC x HILIC is compared to on-line RPLC x RPLC through the separation of peptides. Our choices regarding the conditions are discussed. Injection effects and overloading effects are evaluated in both configurations. It is shown that whereas large volumes can be injected in the second dimension in R...