Kim, Hyun-Soo Jeon, Yoon Jang, Yoon Ok Lee, Ho Shin, Yong Lee, Chang-Woo
Published in
Theranostics
Reversible phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (Pol II) is essential for gene expression control. How altering the phosphorylation of the CTD contributes to gene expression in mammalian systems remains poorly understood. Methods: Primary mouse embryonic fibroblasts, hepatocytes, and embryonic stem cells were isolated...
Huang, Fang Nguyen, Trang Tt Echeverria, Ignacia Ramachandran, Rakesh Cary, Daniele C Paculova, Hana Sali, Andrej Weiss, Arthur Peterlin, Boris Matija Fujinaga, Koh
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Published in
eLife
The positive transcription elongation factor b (P-TEFb) is a critical coactivator for transcription of most cellular and viral genes, including those of HIV. While P-TEFb is regulated by 7SK snRNA in proliferating cells, P-TEFb is absent due to diminished levels of CycT1 in quiescent and terminally differentiated cells, which has remained unexplore...
Nguyen, Duy Buisine, Nicolas Fayol, Olivier Michels, Annemieke A. Bensaude, Olivier Price, David H. Uguen, Patricia
Published in
BMC Molecular and Cell Biology
BackgroundThe 7SK small nuclear RNA (snRNA) found in most metazoans is a key regulator of P-TEFb which in turn regulates RNA polymerase II elongation. Although its primary sequence varies in protostomes, its secondary structure and function are conserved across evolutionary distant taxa.ResultsHere, we describe a novel ncRNA sharing many features c...
Qing, Yingjie Wang, Xiangyuan Wang, Hongzheng Hu, Po Li, Hui Yu, Xiaoxuan Zhu, Mengyuan Wang, Zhanyu Zhu, Yu Xu, Jingyan
...
Published in
Cell Communication and Signaling
BackgroundThe positive transcription elongation factor b (P-TEFb) kinase activity is involved in the process of transcription. Cyclin-dependent kinase 9 (CDK9), a core component of P-TEFb, regulates the process of transcription elongation, which is associated with differentiation and apoptosis in many cancer types. Wogonin, a natural CDK9 inhibitor...
Anshabo, Abel Tesfaye Milne, Robert Wang, Shudong Albrecht, Hugo
Published in
Frontiers in Oncology
Cyclin-dependent kinases (CDKs) are proteins pivotal to a wide range of cellular functions, most importantly cell division and transcription, and their dysregulations have been implicated as prominent drivers of tumorigenesis. Besides the well-established role of cell cycle CDKs in cancer, the involvement of transcriptional CDKs has been confirmed ...
Hasler, Daniele Meister, Gunter Fischer, Utz
Published in
RNA biology
La and La-related proteins (LARPs) are characterized by a common RNA interaction platform termed the La module. This structural hallmark allows LARPs to pervade various aspects of RNA biology. The metazoan LARP7 protein binds to the 7SK RNA as part of a 7SK small nuclear ribonucleoprotein (7SK snRNP), which inhibits the transcriptional activity of ...
Düster, Robert Kaltheuner, Ines H Schmitz, Maximilian Geyer, Matthias
Published in
The Journal of biological chemistry
The concept of liquid-liquid phase separation (LLPS) has emerged as an intriguing mechanism for the organization of membraneless compartments in cells. The alcohol 1,6-hexanediol is widely used as a control to dissolve LLPS assemblies in phase separation studies in diverse fields. However, little is known about potential side effects of 1,6-hexaned...
Feng, Zeming Yang, Zhengrong Gao, Xiang Xue, Yuhua Wang, Xiaohui
Published in
Current HIV research
The latent reservoir of HIV-1 is a major barrier to achieving the eradication of HIV-1/AIDS. One strategy is termed "shock and kill", which aims to awaken the latent HIV-1 using latency reversing agents (LRAs) to replicate and produce HIV-1 particles. Subsequently, the host cells containing HIV-1 can be recognized and eliminated by the immune respo...
Song, Yawei Hu, Gongcheng Jia, Jinping Yao, Mingze Wang, Xiaoshan Lu, Wenliang Hutchins, Andrew P. Chen, Jiekai Ozato, Keiko Yao, Hongjie
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Published in
Frontiers in Molecular Biosciences
The bromodomain-containing protein BRD4 has been thought to transmit epigenetic information across cell divisions by binding to both mitotic chromosomes and interphase chromatin. UV-released BRD4 mediates the recruitment of active P-TEFb to the promoter, which enhances transcriptional elongation. However, the dynamic associations between BRD4 and P...
Fujinaga, Koh Cary, Daniele C.
Published in
Viruses
The final obstacle to achieving a cure to HIV/AIDS is the presence of latent HIV reservoirs scattered throughout the body. Although antiretroviral therapy maintains plasma viral loads below the levels of detection, upon cessation of therapy, the latent reservoir immediately produces infectious progeny viruses. This results in elevated plasma viremi...