Determining the specificities of TALENs, Cas9, and other genome-editing enzymes.
Published in Methods in enzymology
The rapid development of programmable site-specific endonucleases has led to a dramatic increase in genome engineering activities for research and therapeutic purposes. Specific loci of interest in the genomes of a wide range of organisms including mammals can now be modified using zinc-finger nucleases, transcription activator-like effectornucleas...
