We describe a structural and functional study of the G protein-coupled apelin receptor, which binds two endogenous peptide ligands, apelin and Elabela/Toddler (ELA), to regulate cardiovascular development and function. Characterisation of naturally occurring apelin receptor variants from the UK Genomics England 100,000 Genomes Project, and AlphaFol...
PRDM16 is a transcription factor with histone methyltransferase activity expressed at the earliest stages of cardiac development. Pathogenic mutations in humans lead to cardiomyopathy, conduction abnormalities, and heart failure. PRDM16 is specifically expressed in ventricular but not atrial cardiomyocytes, and its expression declines postnatally. ...
Publication status: Published / Chronic fetal hypoxia is one of the most common complications of pregnancy and can programme cardiac abnormalities in adult offspring including ventricular remodelling, diastolic dysfunction and sympathetic dominance. However, the underlying mechanisms at the level of the cardiomyocyte are unknown, preventing the ide...
Peer reviewed: True / Acknowledgements: The authors acknowledge support from the National Genomics Infrastructure in Genomics Production Stockholm funded by Science for Life Laboratory, the Knut and Alice Wallenberg Foundation, the Swedish Research Council, and the SNIC/Uppsala Multidisciplinary Center for Advanced Computational Science for assista...
Funder: Nxera (Sosei Heptares) ORBIT Research Grant Cambridge Biomedical Research Centre Biomedical Resources Grant (University of Cambridge, Cardiovascular Theme, RG64226) / We describe a structural and functional study of the G protein-coupled apelin receptor, which binds two endogenous peptide ligands, apelin and Elabela/Toddler (ELA), to regula...
Acknowledgements: The authors would like to thank Olga Sauchanka (Department of Medicine, University of Cambridge) for surgical advice and assistance throughout this study. / The mitochondrial metabolite succinate is a key driver of ischemia/reperfusion injury (IRI). Targeting succinate metabolism by inhibiting succinate dehydrogenase (SDH) upon re...
Regenerative cardiac tissue is a promising field of study with translational potential as a therapeutic option for myocardial repair after injury, however, poor electrical and contractile function has limited translational utility. Emerging research suggests scaffolds that recapitulate the structure of the native myocardium improve physiological fu...
Acknowledgements: The authors would like to acknowledge Marta Potapo for her assistance with the RNA extractions, library preparation and sequencing, as well as Jennifer Tan, Eleanor Williams, Praveen Anand, and Abel Souza for their advice on transcriptomic data analysis. / Structural cardiotoxicity (SCT) presents a high-impact risk that is poorly ...
Myocardial ischemia-reperfusion (IR) injury may result in cardiomyocyte dysfunction. Mitochondria play a critical role in cardiomyocyte recovery after IR injury. The mitochondrial uncoupling protein 3 (UCP3) has been proposed to reduce mitochondrial reactive oxygen species (ROS) production and to facilitate fatty acid oxidation. As both mechanisms ...
Acknowledgements: We thank the Henan Scope Research Institute of Electrophysiology for providing the Nav1.5 CHO cell line and the MappingLab analysis software for optical mapping, and colleagues who assisted in experimental design, data analyses and manuscript proofreading. / We explored physiological effects of the sodium-glucose co-transporter-2 ...
