Published in Journal of chromatography. A
Recent studies have shown that by combining orthogonal, non-affinity chromatography steps, it is possible to rapidly develop efficient purification processes for molecules of interest. Here, we build upon previous work to develop a flexible framework for identifying resins that remove optimally orthogonal sets of impurities for a wide variety of pr...
Published in Journal of chromatography. A
In this study, the thermodynamics of binding of two industrial mAbs to multimodal cation exchange systems was investigated over a range of buffer and salt conditions via a van't Hoff analysis of retention data. Isocratic chromatography was first employed over a range of temperature and salt conditions on three multimodal resins and the retention da...
This study is comprised of two distinct parts, a chromatographic study and a didactic study.Chromatographic studyThe need for higher selectivity in chromatography purification has increased in recent years. Multimodal resins, offering novel selectivity, are a possible solution to this demand. The purpose of this study is to develop new multimodal r...
Published in Chemical Papers
Multimodal chromatography adsorbents are novel materials with promising properties for downstream processing of therapeutic glycoproteins. This work analyzes the properties of a salt-tolerant membrane, Sartobind STIC, for the purification of recombinant human erythropoietin (rhEPO) produced by human embryonic kidney cells. Batch adsorption experime...
International audience
Published in Journal of chromatography. A
The increasing prevalence of low pI non-mAb therapeutics as well as current challenges in mAb-aggregate separations and low recoveries motivate further development in the multimodal anion exchange (MM AEX) space. In this work, linear salt gradient experiments at pH 7 were used to evaluate the retention of model proteins (with pI from 3.4 to 6.8) in...
Published in Journal of chromatography. A
Although a platform process has been established for purification of antibodies, a deep, fundamental understanding of how these molecules interact with chromatography resins has yet to be developed. The increasing prevalence of antibody-related therapeutics and associated purification challenges further motivate research into these molecular level ...
Published in PDA journal of pharmaceutical science and technology
The process capability and potential for various forms of chromatography to remove viruses have been discussed extensively in the literature, including the observed variability in performance of some unit operations such as Protein A and cation exchange (CEX). Some unit operations such as anion exchange (AEX) have shown robustness over a wide range...
Published in Journal of chromatography. A
Multimodal (MM) chromatography provides a powerful means to enhance the selectivity of protein separations by taking advantage of multiple weak interactions that include electrostatic, hydrophobic and van der Waals interactions. In order to increase our understanding of such phenomena, a computationally efficient approach was developed that combine...
Published in Protein expression and purification
Hydrophobic charge-induction chromatography (HCIC) using 4-mercaptoethylpyridine (4-MEP) as the ligand is used to purify antibodies. The 4-MEP resin ligand has high affinity for antibodies, which makes it difficult to optimize the elution conditions. Recent studies showed that arginine is effective at eluting and purifying antibodies using the HCIC...
