Volpato, Fabiana Lima-Morales, Daiana Wink, Priscila Lamb Willig, Julia de-Paris, Fernanda Ashton-Prolla, Patricia Barth, Afonso Luís
Published in
European Journal of Clinical Microbiology & Infectious Diseases
This study has aimed to evaluate the use pool of samples as a strategy to optimize the diagnostic of SARS-CoV-2 by RT-qPCR. A total of 220 naso/orofaryngeal swab samples were collected and tested using two different protocols of sample pooling. Results from protocol A were identical with the individual results. However, for results from protocol B,...
Figueredo, Lida Jouca de Assis Miranda, Silvana Spíndola de Santos, Lucas Benício Dos Manso, Caroline Gontijo Gonçalves Soares, Valéria Martins Alves, Suely Vater, Maria Cláudia Kritski, Afrânio Lineu Carvalho, Wânia da Silva Pádua, Cristiane Menezes de
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Published in
Revista da Sociedade Brasileira de Medicina Tropical
Rapid and accurate tuberculosis detection is critical for improving patient diagnosis and decreasing tuberculosis transmission. Molecular assays can significantly increase laboratory costs; therefore, the average time and economic impact should be evaluated before implementing a new technology. The aim of this study was to evaluate the cost and ave...
Bonini, Andrea Poma, Noemi Vivaldi, Federico Kirchhain, Arno Salvo, Pietro Bottai, Daria Tavanti, Arianna Di Francesco, Fabio
Published in
Journal of Pharmaceutical and Biomedical Analysis
A main challenge in the development of biosensing devices for the identification and quantification of nucleic acids is to avoid the amplification of the genetic material from the sample by polymerase chain reaction (PCR), which is at present necessary to enhance sensitivity and selectivity of assays. PCR has undoubtedly revolutionized genetic anal...
Rocha, Daniele de Melo, Gisely Cardoso Carneiro, José Marcelo Hipólito Ribeiro, Marisa Ribeiro, Sthefanie de Godoy, Daniela Tupy de Almeida, Anne Cristine Gomes de Andrade, Elisabete Ferreira Abrahim, Cláudia Maria de Moura Fraiji, Nelson Abrahim
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Published in
Malaria Journal
BackgroundMalaria can be transmitted by blood transfusion through donations collected from asymptomatic donors. Transfusion-transmitted malaria (TTM) poses a great risk to blood services worldwide. A good screening tool for Plasmodium spp. detection in blood banks must have a high sensitivity for prevention of TTM. However, in Brazilian blood banks...
de Lucca Maganha, Samara Rita Cardoso, Pedro Henrique Magalhães de Carvalho Balian, Simone de Almeida-Queiroz, Sabrina Ribeiro Fernandes, Andrezza Maria de Sousa, Ricardo Luiz Moro
Published in
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
The aim of this study is to report the occurrence of Lymphocystivirus in Brazilian ornamental fish. From 25 ornamental fish species submitted for molecular diagnosis, only one sample (Pomacanthus imperator) was positive, and its viral nucleotide sequence obtained clustered with sequences of genotype VII. To our knowledge, this is the first report o...
Pillonel, Trestan Scherz, Valentin Jaton, Katia Greub, Gilbert Bertelli, Claire
Published in
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
Figueredo, Lida Jouca de Assis Miranda, Silvana Spíndola de Santos, Lucas Benício dos Manso, Caroline Gontijo Gonçalves Soares, Valéria Martins Alves, Suely Vater, Maria Cláudia Kritski, Afrânio Lineu Carvalho, Wânia da Silva Pádua, Cristiane Menezes de
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Abstract INTRODUCTION: Rapid and accurate tuberculosis detection is critical for improving patient diagnosis and decreasing tuberculosis transmission. Molecular assays can significantly increase laboratory costs; therefore, the average time and economic impact should be evaluated before implementing a new technology. The aim of this study was to ev...
Pellino, Antonio Riello, Erika Nappo, Floriana Brignola, Stefano Murgioni, Sabina Djaballah, Selma Ahcene Lonardi, Sara Zagonel, Vittorina Rugge, Massimo Loupakis, Fotios
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Published in
World journal of gastroenterology
Gastric cancer (GC) represents a leading cause of cancer related morbidity and mortality worldwide accounting for more than 1 million of newly diagnosed cases and thousands of deaths every year. In the last decade, the development of targeted therapies and the optimization of already available chemotherapeutic drugs has expanded the available treat...
Kim, Dong Woo Chun, Hyeong Jin Kim, Jae-Ho Yoon, Hyunjin Yoon, Hyun C.
Published in
Nano Convergence
The detection of foodborne pathogenic microorganisms is an essential issue in molecular diagnostics. Fluorescence-based assays have been widely utilized in molecular diagnostics because of their ability to detect and measure low analyte concentrations. However, conventional fluorescence-based assays require sophisticated optics systems, such as a s...
Jousset, Agnès B Bernabeu, Sandrine Bonnin, Remy A Creton, Elodie Cotellon, Garance Sauvadet, Aimie Naas, Thierry Dortet, Laurent
Published in
International journal of antimicrobial agents
Plasmid-mediated colistin resistance is increasingly described worldwide in Enterobacteriaceae from animal and human isolates. Diffusion of these resistance traits among carbapenem-resistant enterobacterial isolates is of particular concern as colistin has become the last resort antibiotic for treating human infections with these organisms. Therefo...
