The rapid growth of computer power has allowed development of ray tracing software to a point where simulation appears as real as reality, at least at first glance. Very many packages are available, probably in the hundreds, and still in the dozens if we consider only open source software. However, most software, devoted to scene rendering for vide...
Published in Heliyon
Xylia evansii is widely used in traditional medicine to stop bleeding gums and treat wounds. This study was undertaken to assess the wound healing activity and toxicity profile of the stem bark methanol extract of X. evansii (XES). Wound healing activity was determined by the dermal excision model in rats. The free radical scavenging capacity, anti...
Published in STAR protocols
Transcription factors (TFs) play a pivotal role in gene expression, and their DNA binding is the prerequisite to instigating gene transcription. Here, we present a protocol that exploits the proximity ligation assay technique to measure the DNA-binding activities of TFs in situ at the single-cell resolution. We describe steps for immunostaining wit...
This dataset corresponds to the final figures from ‘Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity’ by James Rowe, Mathieu Grangé-Guermente, Marino Exposito-Rodriguez, Rinukshi Wimalasekera, Martin O. Lenz, Kartika N. Shetty, Sean R. Cutler, Alexander M. Jones. Data was collected at Sainsbu...
peer reviewed / Trypanosomiasis, a neglected tropical disease (NTD), challenges communities in sub-Saharan Africa and Latin America. The World Health Organization underscores the need for practical, field-adaptable diagnostics and rapid screening tools to address the negative impact of NTDs. While artificial intelligence has shown promising results...
Polarized and wide-field light microscopy has been studied for many years to develop accurate information-rich images within a focused framework on biophysics biomedicine. Technological advances conceptual understanding have recently led significant results in terms of applications. Simultaneously, developments label-free methods are opening new wi...
Published in STAR protocols
Adherens junctions (AJs) are multi-protein adhesion structures that couple contractile actomyosin networks of epithelial cells within a tissue. Here, we present an epithelial cell spreading assay on E-cadherin-coated glass or polydimethylsiloxane (PDMS) substrates for detailed microscopy-based analysis of cadherin adhesions. We describe steps for p...
Published in Clinics in orthopedic surgery
The application of biportal endoscopic spinal surgery (BESS) in spine surgery is increasing. However, the clinical results of related studies have been inconsistent. In this study, the perioperative and clinical outcomes of two techniques in single-level lumbar decompression surgery were compared using the perspective of a spine surgeon experienced...
Published in Nature
Despite ongoing efforts to prevent the damaging effects of alkali-silica reaction (ASR) in concrete structures, this phenomenon still exists and causes significant degradation. This study aims to investigate the role of various deicing agents in provoking ASR by examining volume expansion, cracks, and microstructure of mortar and concrete containin...
