Published in Journal of bioscience and bioengineering
Microcarriers provide a high surface-area-to-volume ratio that can realize high yields of cell products, including human mesenchymal stem cells (hMSCs). Here, we report a novel poly(vinyl alcohol) (PVA)-based microcarrier for hMSC expansion in suspension culture. PVA microcarriers were prepared as collagen-coated PVA hydrogels 181 μm in size and a ...
Published in Cell Journal (Yakhteh)
Objective: Umbilical cord blood (UCB) is an accessible and effective alternative source for hematopoietic stem cell (HSC) transplantation. Although the clinical application of UCB transplantation has been increased recently, quantitative limitation of HSCs within a single cord blood unit still remains a major hurdle for UCB transplantation. In this...
Published in Bioactive Materials
• The desired photocurable FN-HAMA microcarriers could mimic the polysaccharide/peptide components of stem niches. • The FN-HAMA microcarriers could enhance the bioactivities and pro-regenerative capabilities of the laded therapeutic ADSCs. • The FN-HAMA microcarriers show great potential in diabetic wound treatment.
Published in Stem cell reviews and reports
Although various reconstruction techniques are available for cartilage defects, the repair effects and conveniences remain to be further improved due to the limited regenerative capacity of cartilaginous tissues and difficulties in seamlessly fulfilling irregularly shaped defects. In the current study, we explored the repair efficacy of stem cell m...
peer reviewed / Microcarriers (MCs, typically 50–200 µm) are promising growth supports for high-throughput cell expansion, with capability to overcome the limitations of surface area availability and nutrient access encountered by cell culture in 2D well plate configurations. Equipping MCs with in-built capability to sense molecular biomarkers is a...
To date, bottlenecks persist concerning deep scientific understanding of how various process parameters will impact the Mesenchymal Stem Cell production. Specifically applied to microcarrier-based expansion processes of WJ-MSC's, very little information is available to characterize the impact of microcarrier concentration on MSC growth and death ra...
Published in Transplant immunology
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors that threaten human health; thus, the establishment of an animal model with clinical features similar to human hepatocellular carcinoma is of important practical significance. Taking advantage of the novel microcarrier-6, human HCC cells were injected into immunocompetent mic...
Published in Journal of Nanobiotechnology
Loss of extracellular matrix (ECM) of cartilage due to oxidative stress injury is one of the main characteristics of osteoarthritis (OA). As a bioactive molecule derived from the traditional Chinese Burdock , arctiin exerts robust antioxidant properties to modulate redox balance. However, the potential therapeutic effects of arctiin on OA and the u...
Published in Cytotechnology
Human umbilical cord mesenchymal stem/stromal cells (hUC-MSCs) have attracted significant research interests in regenerative medicine and cell-based therapies due to their minimally invasive isolation procedure, abundant availability, allogenic nature, improved proliferation capacity and tri-lineage differentiation potential. However, the challenge...
Published in Methods in molecular biology (Clifton, N.J.)
In recent years, the use of hMSCs, which may be isolated from adipose tissue among others, for the treatment of diseases has increased significantly. The cell quantities required for such therapeutic approaches, between 1012 and 1013, have thus far been predominantly produced using commercially available multi-tray systems, such as the Cell Factory...
