Zhu, Jingjing Chen, Rongrong Zhao, Shuqi Zhu, Lixia Li, Xueying Xie, Mixue Ye, Xiujin
Published in
Platelets
The first-line therapy for primary immune thrombocytopenia (ITP) is steroids, but about one-third of patients do not respond to steroids. Recent studies have shown megakaryocyte (MK) growth and development abnormalities and poorly compensated thrombopoiesis. Here, we attempted to determine the impact of MK morphological classification on steroid re...
Valdivia-Mazeyra, Mariel F. Salas, Clara Nieves-Alonso, Jesús M. Martín-Fragueiro, Luz Bárcena, Carmen Muñoz-Hernández, Patricia Villar-Zarra, Karen Martín-López, Javier Ramasco-Rueda, Fernando Fraga, Javier
...
Published in
Virchows Archiv
Pulmonary megakaryocytes participate in the pathogenesis of lung damage, particularly in acute lung injury. Although megakaryocytes are not mentioned as a characteristic histologic finding associated to pulmonary injury, a few studies reveal that their number is increased in diffuse alveolar damage (DAD). In this autopsy study, we have observed a r...
Mallah, Haneen Ball, Somedeb Sekhon, Jasmine Parmar, Kanak Nugent, Kenneth
Published in
Respiratory medicine
Platelets are essential mediators of inflammation and thrombosis. Chronic obstructive pulmonary disease (COPD) is a heterogeneous multisystem disease, causing significant morbidity and mortality worldwide. Recent evidence suggests that the lung is an important organ for platelet biogenesis. Cigarette smoking has been shown to induce platelet aggreg...
Wang, Manman Zhang, Tan Zhang, Xuechun Jiang, Zhou Peng, Min Huang, Zan
Published in
Cell & Bioscience
BackgroundForced polyploidization is an effective strategy for acute megakaryoblastic leukemia (AMKL) therapy and factors controlling polyploidization are potential targets for drug development. Although bone morphology protein 2-inducible kinase (BMP2K) has been implied to be a potential target for fasudil, a potent polyploidy-inducing compound, t...
Psaila, B Wang, G Rodriguez-Meira, A Li, R Heuston, EF Murphy, L Yee, D Hitchcock, IS Sousos, N O'Sullivan, J
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Myelofibrosis is a severe myeloproliferative neoplasm characterized by increased numbers of abnormal bone marrow megakaryocytes that induce fibrosis, destroying the hematopoietic microenvironment. To determine the cellular and molecular basis for aberrant megakaryopoiesis in myelofibrosis, we performed single-cell transcriptome profiling of 135,929...
Dong, Fang Hao, Sha Zhang, Sen Zhu, Caiying Cheng, Hui Yang, Zining Hamey, Fiona K Wang, Xiaofang Gao, Ai Wang, Fengjiao
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How transplanted haematopoietic stem cells (HSCs) reside and behave in a preconditioned host at the early stage has not been studied due to technical limitations. Here, by single-cell RNA-sequencing, we first obtained the transcriptome-based classifications of 28 haematopoietic cell types, and then applied them in conjunction with functional assays...
Woods, Brittany Chen, Wei Chiu, Sophia Marinaccio, Christian Fu, Chunling Gu, Lilly Bulic, Marinka Yang, Qiong Zouak, Anouar Jia, Shengxian
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Published in
Clinical cancer research : an official journal of the American Association for Cancer Research
The myeloproliferative neoplasms (MPN), including polycythemia vera, essential thrombocythemia, and primary myelofibrosis, are characterized by the expansion of the erythroid, megakaryocytic, and granulocytic lineages. A common feature of these disorders is the presence of abnormal megakaryocytes, which have been implicated as causative agents in t...
Maupin, Kevin A Himes, Evan R Plett, Artur P Chua, Hui Lin Singh, Pratibha Ghosh, Joydeep Mohamad, Safa F Abeysekera, Irushi Fisher, Alexa Sampson, Carol
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Published in
Bone
Osteoblast number and activity decreases with aging, contributing to the age-associated decline of bone mass, but the mechanisms underlying changes in osteoblast activity are not well understood. Here, we show that the age-associated bone loss critically depends on impairment of the ability of megakaryocytes (MKs) to support osteoblast proliferatio...
Mahaut Smith, Martyn P Evans, Richard J Vial, Catherine
Published in
Purinergic signalling
A P2X1-eYFP knock-in mouse was generated to study receptor expression and mobility in smooth muscle and blood cells. eYFP was added to the C-terminus of the P2X1R and replaced the native P2X1R. Fluorescence corresponding to P2X1-eYFPR was detected in urinary bladder smooth muscle, platelets and megakaryocytes. ATP-evoked currents from wild type and...
Li, Yunqian Guo, Ran Wang, Lili Li, Shanshan Zhu, Zhixiang Tu, Pengfei
Published in
Biochemical pharmacology
Hematopoietic stem cell transplantation (HSCT) plays an important role in the therapy of hematological malignancies and some nonmalignant diseases. Granulocyte colony-stimulating factor (G-CSF) is generally used to mobilize and collect hematopoietic stem cells from donors and accelerate neutrophil recovery in transplantation recipients. However, le...
