Sun, Ping Zhou, Yue Qiu, Tong Peng, Jian
Published in
Analytical and bioanalytical chemistry
Laccase is an enzyme known for its eco-friendly uses in environmental cleanup and biotechnology. However, it has limitations such as low stability, high cost, and complex recycling. So, there is a need for laccase mimics that can effectively imitate its properties. Herein, we created copper formate-lysine nanoparticles (Cuf-Lys) that mimic laccase'...
Son, Ahrum Kim, Hyunsoo Diedrich, Jolene Bamberger, Casimir McClatchy, Daniel Lipton, Stuart Yates, John
Mass spectrometry-based methods can provide a global expression profile and structural readout of proteins in complex systems. Preserving the in vivo conformation of proteins in their innate state is challenging during proteomic experiments. Here, we introduce a whole animal in vivo protein footprinting method using perfusion of reagents to add dim...
Jensen, Steven J Cuthbert, Bonnie J Garza-Sánchez, Fernando Helou, Colette C de Miranda, Rodger Goulding, Celia W Hayes, Christopher S
Advanced glycation end-products (AGE) are a pervasive form of protein damage implicated in the pathogenesis of neurodegenerative disease, atherosclerosis and diabetes mellitus. Glycation is typically mediated by reactive dicarbonyl compounds that accumulate in all cells as toxic byproducts of glucose metabolism. Here, we show that AGE crosslinking ...
Moon, Brianna Zhou, Iris Ning, Yingying Chen, Yin-Ching Le Fur, Mariane Shuvaev, Sergey Akam, Eman Ma, Hua Solsona, Cesar Weigand-Whittier, Jonah
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BACKGROUND: Aging-associated left ventricular dysfunction promotes cardiopulmonary fibrogenic remodeling, Group 2 pulmonary hypertension (PH), and right ventricular failure. At the time of diagnosis, cardiac function has declined, and cardiopulmonary fibrosis has often developed. Here, we sought to develop a molecular positron emission tomography (...
Willnow, Philipp; 168519; Teleman, Aurelio A.;
Histone acetylation regulates gene expression, cell function and cell fate1. Here we study the pattern of histone acetylation in the epithelial tissue of the Drosophila wing disc. H3K18ac, H4K8ac and total lysine acetylation are increased in the outer rim of the disc. This acetylation pattern is controlled by nuclear position, whereby nuclei contin...
Dunkelmann, Daniel L Chin, Jason W
Publication status: Published / Over the past 16 years, genetic code expansion and reprogramming in living organisms has been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs. Here we summarize the discovery of the pyrrolysine system and describe the unique properties of PylRS/tRNAPyl p...
Boytsov, Danila Madej, Gregor M Horn, Georg Blaha, Nadine Köcher, Thomas Sitte, Harald H Siekhaus, Daria Ziegler, Christine Sandtner, Walter Roblek, Marko
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Orphan solute carrier (SLC) represents a group of membrane transporters whose exact functions and substrate specificities are not known. Elucidating the function and regulation of orphan SLC transporters is not only crucial for advancing our knowledge of cellular and molecular biology but can potentially lead to the development of new therapeutic s...
McManus, David Patton, Andrew P Smyllie, Nicola J Chin, Jason W Hastings, Michael H
Publication status: Published / Funder: UK Research and Innovation; doi: http://dx.doi.org/10.13039/100014013 / The biological clock of the suprachiasmatic nucleus (SCN) orchestrates circadian (approximately daily) rhythms of behaviour and physiology that underpin health. SCN cell-autonomous time-keeping revolves around a transcriptional/translatio...
Wang, Zhaoning Ren, Bing
Methylation of histone H3 on the lysine-4 residue (H3K4me) is found throughout the eukaryotic domain, and its initial discovery as a conserved epigenetic mark of active transcription from yeast to mammalian cells has contributed to the histone code hypothesis. However, recent studies have raised questions on whether the different forms of H3K4me pl...
Longin, Hannelore; 148057; Broeckaert, Nand; 105117; Langen, Maarten; 138113; Hari, Roshan; Kramarska, Anna; Oikarinen, Kasper; Hendrix, Hanne; 99296; Lavigne, Rob; 24787; van Noort, Vera; 89822;
SUMMARY: Today, hundreds of post-translational modification (PTM) sites are routinely identified at once, but the comparison of new experimental datasets to already existing ones is hampered by the current inability to search most PTM databases at the protein residue level. We present FLAMS (Find Lysine Acylations and other Modification Sites), a P...
