Morales-Palomo, Sergio Liras, Marta González-Fernández, Cristina Tomás-Pejó, Elia
Published in
Biotechnology for Biofuels and Bioproducts
Background Microbial lipids are found to be an interesting green alternative to expand available oil sources for the chemical industry. Yeasts are considered a promising platform for sustainable lipid production. Remarkably, some oleaginous yeasts have even shown the ability to grow and accumulate lipids using unusual carbon sources derived from or...
Li, Wen Wang, Xiaobin Zhang, Xianbin Gong, Peng Ding, Degang Wang, Ning Wang, Zhifeng
Published in
Lipids in Health and Disease
BackgroundThe high drug resistance and metabolic reprogramming of clear cell renal cell carcinoma (ccRCC) are considered responsible for poor prognosis. In-depth research at multiple levels is urgently warranted to illustrate the lipid composition, distribution, and metabolic pathways of clinical ccRCC specimens.MethodsIn this project, a leading-ed...
Tero, Benjamin W. Fortier, Bethany Soucy, Ashley N. Paquette, Ginger Liaw, Lucy
Published in
Journal of Vascular Research
Quantification of adipocyte size and number is routinely performed for white adipose tissues using existing image analysis software. However, thermogenic adipose tissue has multilocular adipocytes, making it difficult to distinguish adipocyte cell borders and to analyze lipid proportion using existing methods. We developed a simple, standardized me...
Phan, Minh Anh Thu Madigan, Michele C Willcox, Mark Stapleton, Fiona Golebiowski, Blanka
Published in
Experimental eye research
Two spectrophotometric microplate assays with dual staining for either fluorescent Nile red (NR) plus 4,6-diamidino-2-phenylindole (DAPI) or non-fluorescent Oil red O (ORO) plus Crystal violet (CV) were applied and optimised to evaluate the lipid producing capacity of immortalised human meibomian gland epithelial cells (iHMGEC). Cells were treated ...
Seow, Pohchoo Narayanan, Vairavan Romelean, Ronie J Wong, Jeannie Hsiu Ding Win, Myint Tun Chandran, Hari Chinna, Karuthan Rahmat, Kartini Ramli, Norlisah
Published in
Academic radiology
Our study evaluated the capability of magnetic resonance imaging in- and opposed-phase (IOP) derived lipid fraction as a novel prognostic biomarker of survival outcome in glioma. We analyzed 46 histologically proven glioma (WHO grades II-IV) patients using standard 3T magnetic resonance imaging brain tumor protocol and IOP sequence. Lipid fraction ...
Chmielarz, Mikołaj Sampels, Sabine Blomqvist, Johanna Brandenburg, Jule Wende, Frida Sandgren, Mats Passoth, Volkmar
Published in
Biotechnology for Biofuels
BackgroundLipid extraction for quantification of fat content in oleaginous yeasts often requires strong acids and harmful organic solvents; it is laborious and time-consuming. Therefore, in most cases just endpoint measurements of lipid accumulation are performed and kinetics of intracellular lipid accumulation is difficult to follow. To address th...
Patel, Alok Antonopoulou, Io Enman, Josefine Rova, Ulrika Christakopoulos, Paul Matsakas, Leonidas
Published in
BMC Chemical Engineering
Oleaginous microorganisms are among the most promising feedstocks for the production of lipids for biofuels and oleochemicals. Lipids are synthesized in intracellular compartments in the form of lipid droplets. Therefore, their qualitative and quantitative analysis requires an initial pretreatment step that allows their extraction. Lipid extraction...
Koelmel, Jeremy P. Cochran, Jason A. Ulmer, Candice Z. Levy, Allison J. Patterson, Rainey E. Olsen, Berkley C. Yost, Richard A. Bowden, John A. Garrett, Timothy J.
Published in
BMC Bioinformatics
BackgroundLipidomics, the comprehensive measurement of lipids within a biological system or substrate, is an emerging field with significant potential for improving clinical diagnosis and our understanding of health and disease. While lipids diverse biological roles contribute to their clinical utility, the diversity of lipid structure and concentr...
Encarnação, Telma Arranja, Cláudia T. Cova, Tânia F. G. G. Pais, Alberto A. C. C. Campos, Maria G. Sobral, Abílio J. F. N. Burrows, Hugh D.
Published in
Journal of Applied Phycology
A simple reliable method with fast response for lipid detection and quantification is proposed, combining a new highly lipophilic fluorescent probe BODIPY BD-C12 and image analysis to determine the algal lipid content and the lipid production in the microalgae Nannochloropsis sp. Lipid bodies stained with BODIPY BD-C12 have a characteristic multico...
Elmer-Dixon, Margaret M Bowler, Bruce E
Published in
Analytical biochemistry
A novel approach to quantification of cardiolipin and DOPC lipid and vesicle concentration that is rapid and inexpensive is described. Traditional approaches to quantifying vesicle concentration destroy sample and are often time consuming. Using common laboratory equipment and software, lipid vesicles were reliably quantified allowing for immediate...
