Published in Glycoconjugate Journal
Plants contain an extended group of lectins differing from each other in their molecular structures, biochemical properties and carbohydrate-binding specificities. The heterogeneous group of plant lectins can be classified in several families based on the primary structure of the lectin domain. All proteins composed of one or more lectin domains, o...
Published in Microchimica Acta
A competitive strategy for glycan determination on cell surface with organic electrochemical transistors (OECTs) has been developed. The carboxylic multi-wall carbon nanotubes were firstly immobilized on the gate interface to cross-link the specific monose with adipic dihydrazide as the linker, which could then competitively recognize horseradish p...
Published in Histochemistry and Cell Biology
Wild-type lectins have distinct types of modular design. As a step to explain the physiological importance of their special status, hypothesis-driven protein engineering is used to generate variants. Concerning adhesion/growth-regulatory galectins, non-covalently associated homodimers are commonly encountered in vertebrates. The homodimeric galecti...
Published in Theriogenology
The high viscosity of Camelidae semen continues to present a major impediment for its application in assisted reproduction technology. The exposure of epididymal spermatozoa (ES) to seminal plasma (SP) may provide an approach to enhance the development of assisted reproductive techniques in these important domestic species. Since the sperm glycocal...
Calreticulin (Calr) is an endoplasmic reticulum (ER) chaperone involved in protein quality control, Ca2+ regulation and other cellular processes. The structure of Calr is unusual, reflecting different functions of the protein: a proline-rich β-hairpin arm and an acidic C-terminal tail protrude from a globular core, composed of a β-sheet sandwich an...
Lectins are ubiquitous proteins that can reversibly and specifically bind to carbohydrates. Carbohydrate-mediated recognition and adhesion are key events in the early steps of the interaction of pathogenic or symbiotic bacteria with a host. For example, Pseudomonas aeruginosa is an opportunistic bacterium that causes lethal lung infection in cystic...
For an equilibrated survival of humans against bacterial infections, immune cells have a well-defined function to distinguish between self and non-self structures. Escherichia coli is a Gram-negative bacterium that reside in the gut microbiota, either as a beneficial or harmful microorganism.The tolerance of pathogenic strains or their clearance is...
Published in Experimental Cell Research
COVID-19 was declared an international public health emergency in January, and a pandemic in March of 2020. There are over 125 million confirmed COVID-19 cases that have caused over 2.7 million deaths worldwide as of March 2021. COVID-19 is caused by the SARS-CoV-2 virus. SARS-CoV-2 presents a surface “spike” protein that binds to the ACE2 receptor...
Published in Journal of Molecular Modeling
The biomolecular recognition of D-mannose-binding lectin from Artocarpus heterophyllus (ArtinM) by Horseradish Peroxidase (HRP) mediated by glycosylation allows their application in a multitude of biological systems. The present work describes the use of molecular dynamics (MD) to assess the Gibbs free energy associated with the formation of a Arti...
Bioinformatics uses mathematical concepts and informatics tools to unravel the knowledge hidden in biological data. When bioinformatics is applied to glycans and glycobiology, it is called glyco-informatics. New technologies allow mass sequencing of new species genomes and of environmental samples metagenomes. But all newly discovered genomes and e...
