Shi, Ji Tong, Yiping
Published in
Frontiers in Plant Science
Understanding the molecular mechanisms in wheat response to nitrogen (N) fertilizer will help us to breed wheat varieties with improved yield and N use efficiency. Here, we cloned TaLAMP1-3A , -3B , and -3D , which were upregulated in roots and shoots of wheat by low N availability. In a hydroponic culture, lateral root length and N uptake were dec...
Lee, Duk-Shin Kim, Ji-Eun
Published in
International Journal of Molecular Sciences
Recently, we have reported that blockade/deletion of P2X7 receptor (P2X7R), an ATP-gated ion channel, exacerbates heat shock protein 25 (HSP25)-mediated astroglial autophagy (clasmatodendrosis) following kainic acid (KA) injection. In P2X7R knockout (KO) mice, prolonged astroglial HSP25 induction exerts 5′ adenosine monophosphate-activated protein ...
Girsch, James H Jackson, Wallen Carpenter, John E Moninger, Thomas O Jarosinski, Keith W Grose, Charles
Published in
Journal of virology
The literature on the egress of different herpesviruses after secondary envelopment is contradictory. In this report, we investigated varicella-zoster virus (VZV) egress in a cell line from a child with Pompe disease, a glycogen storage disease caused by a defect in the enzyme required for glycogen digestion. In Pompe cells, both the late autophagy...
Chukwudozie, Onyeka S.
Published in
Access Microbiology
Lassa virus (LASV) is a single-stranded RNA virus that has plagued the Sub-Saharan part of Africa, precisely Nigeria where various pathogenic strains with varied genomic isoforms have been identified. The human lysosomal-associated membrane protein 1 (LAMP1) is alternately required for the micropinocytosis of LASV. Therefore, it is of interest to u...
Heda, Ghanshyam D Shrestha, Lisa Thapa, Sagarina Ghimire, Shreya Raut, Diptika
Published in
BioTechniques
Protein samples electroblotted onto nitrocellulose membranes and quenched with a mixture of blocking agents produced a strong signal for cystic fibrosis transmembrane-conductance regulator (CFTR), a high-molecular-weight protein, in western blotting. Optimized conditions for CFTR were then extended to medium- and low-molecular-weight proteins (LAMP...
Baba, Kosuke Kuwada, Sara Nakao, Ayaka Li, Xuebing Okuda, Naoaki Nishida, Ai Mitsuda, Satoshi Fukuoka, Natsuki Kakeya, Hideaki Kataoka, Takao
...
Published in
Histochemistry and cell biology
Lysosomal-associated membrane protein 1 (LAMP1) mainly localizes to lysosomes and late endosomes. We herein investigated the intracellular localization of lysosomal membrane proteins in five mammalian cultured cell lines. Rat LAMP1 fused to enhanced green fluorescent protein (EGFP) mostly accumulated at a particular cytoplasmic area and barely co-l...
Panda, Prashanta Kumar Patra, Srimanta Naik, Prajna Paramita Praharaj, Prakash Priyadarshi Mukhopadhyay, Subhadip Meher, Biswa Ranjan Gupta, Piyush Kumar Verma, Rama S Maiti, Tapas K Bhutia, Sujit K
...
Published in
Journal of cellular physiology
Therapy-induced senescence in cancer cells is an irreversible antiproliferative state, which inhibits tumor growth and is therefore a potent anti-neoplastic mechanism. In this study, low doses of Abrus agglutinin (AGG)-induced senescence through autophagy in prostate carcinoma cells (PC3) and inhibited proliferation. The inhibition of autophagy wit...
Kyriakou, Kalia W. Lederer, Carsten Kleanthous, Marina Drousiotou, Anthi Malekkou, Anna
Published in
International Journal of Molecular Sciences
The ASAH1 gene encodes acid ceramidase (AC), an enzyme that is implicated in the metabolism of ceramide (Cer). Mutations in the ASAH1 gene cause two different disorders, Farber disease (FD), a rare lysosomal storage disorder, and a rare form of spinal muscular atrophy combined with progressive myoclonic epilepsy (SMA-PME). In the absence of human i...
Tingting, Chen Shizhou, Yang Songfa, Zhang Junfen, Xu Weiguo, Lu Xiaodong, Cheng Xing, Xie
Published in
Cancer medicine
Although the role of high-risk human papillomavirus (HPV) E6 and E7 in cellular malignant transformation has been elucidated, the function of both genes in cellular homeostasis is still unknown. Autophagy functions in maintenance of cellular homeostasis play a key role in the initiation and development of cancer and infectious disease. Cervical can...
Wang, Xiaoping Li, Chun Wang, Qiyan Li, Weili Guo, Dongqing Zhang, Xuefeng Shao, Mingyan Chen, Xu Ma, Lin Zhang, Qian
...
Published in
Cancers
Clinical use of the anti-cancer drug doxorubicin (DOX) is largely limited due to its severe cardiotoxicity. Dysregulation of autophagy is implicated in DOX-induced cardiotoxicity (DIC). Prior studies have indicated that Beclin1 and lysosomal-associated membrane proteins-1 (LAMP1) are critical mediators of autophagy. In this work, by assessing autop...