Li, Lu Wu, You Zhao, Chenhua Miao, Yiyang Cai, Jing Song, Lingyun Wei, Jing Chakraborty, Tapas Wu, Limin Wang, Deshou
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Published in
The Journal of steroid biochemistry and molecular biology
Sex steroids play critical roles in sex differentiation and gonadal development in teleosts. Steroidogenic acute regulatory protein (StAR), transporting cholesterol (the substrate for steroidogenesis) from the outer mitochondrial membrane to the inner membrane, is the first rate-limiting factor of steroidogenesis. Interestingly, two StAR genes (nam...
Blaze, J Navickas, A Phillips, HL Heissel, S Plaza-Jennings, A Miglani, S Asgharian, H Foo, M Katanski, CD Watkins, CP
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Epitranscriptomic mechanisms linking tRNA function and the brain proteome to cognition and complex behaviors are not well described. Here, we report bi-directional changes in depression-related behaviors after genetic disruption of neuronal tRNA cytosine methylation, including conditional ablation and transgene-derived overexpression of Nsun2 in th...
Giuliani, Virginia Miller, Meredith A Liu, Chiu-Yi Hartono, Stella R Class, Caleb A Bristow, Christopher A Suzuki, Erika Sanz, Lionel A Gao, Guang Gay, Jason P
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Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer that has remained clinically challenging to manage. Here we employ an RNAi-based in vivo functional genomics platform to determine epigenetic vulnerabilities across a panel of patient-derived PDAC models. Through this, we identify protein arginine methyltransferase 1 (PRMT1) as a criti...
Santi, Ana Maria Murta Silva, Paula Alves Santos, Isabella Fernandes Martins Murta, Silvane Maria Fonseca
Published in
Parasites & Vectors
BackgroundSuperoxide dismutase (SOD), a central component of the antioxidant defence system of most organisms, removes excess superoxide anions by converting them to oxygen and hydrogen peroxide. As iron (Fe) SOD is absent in the human host, this enzyme is a promising molecular target for drug development against trypanosomatids.ResultsWe obtained ...
Chen, Wei-Ching To, Minh D Westcott, Peter MK Delrosario, Reyno Kim, Il-Jin Philips, Mark Tran, Quan Bollam, Saumya R Goodarzi, Hani Bayani, Nora
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The commonly mutated human KRAS oncogene encodes two distinct KRAS4A and KRAS4B proteins generated by differential splicing. We demonstrate here that coordinated regulation of both isoforms through control of splicing is essential for development of Kras mutant tumors. The minor KRAS4A isoform is enriched in cancer stem-like cells, where it respond...
Sahoo, Debashis Swanson, Lee Sayed, Ibrahim M Katkar, Gajanan D Ibeawuchi, Stella-Rita Mittal, Yash Pranadinata, Rama F Tindle, Courtney Fuller, Mackenzie Stec, Dominik L
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Modeling human diseases as networks simplify complex multi-cellular processes, helps understand patterns in noisy data that humans cannot find, and thereby improves precision in prediction. Using Inflammatory Bowel Disease (IBD) as an example, here we outline an unbiased AI-assisted approach for target identification and validation. A network was b...
Chen, Peng Zeng, Ji Liu, Zheng Thaker, Hatim Wang, Siyu Tian, Songhai Zhang, Jie Tao, Liang Gutierrez, Craig B Xing, Li
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C. difficile is a major cause of antibiotic-associated gastrointestinal infections. Two C. difficile exotoxins (TcdA and TcdB) are major virulence factors associated with these infections, and chondroitin sulfate proteoglycan 4 (CSPG4) is a potential receptor for TcdB, but its pathophysiological relevance and the molecular details that govern recog...
Hu, Lina Feng, Shuying Liang, Gaofeng Du, Jingxia Li, Aifang Niu, Chunling
Published in
AMB Express
Dunaliella salina (D. salina) has been exploited as a novel expression system for the field of genetic engineering. However, owing to the low or inconsistent expression of target proteins, it has been greatly restricted to practical production of recombinant proteins. Since the accurate gene editing function of clustered regularly interspaced short...
Wang, Ming Sun, ZhaoLin Ding, Fangrong Wang, Haiping Li, Ling Li, Xue Zheng, Xianjin Li, Ning Dai, Yunping Wu, Changxin
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Published in
Cellular and Molecular Life Sciences
Functional elucidation of bovine Y-chromosome genes requires available genome editing technologies. Meanwhile, it has yet to be proven whether the bovine Sry gene is the main or single factor involved in the development of the male phenotype in bovine. Here, we efficiently knocked out four Y-linked genes ( Sry , ZFY , DDX3Y , and EIF2S3Y ) in bovin...
Mullins, Linda Ivy, Jessica Ward, Mairi Tenstad, Olav Wiig, Helge Kitada, Kento Manning, Jon Rakova, Natalia Muller, Dominik Mullins, John
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Published in
Pflugers Archiv
We discovered high Na+ and water content in the skin of newborn Sprague–Dawley rats, which reduced ~ 2.5-fold by 7 days of age, indicating rapid changes in extracellular volume (ECV). Equivalent changes in ECV post birth were also observed in C57Bl/6 J mice, with a fourfold reduction over 7 days, to approximately adult levels. This established the ...
