Yin, Yajun Hao, Haiyang Xu, Xingbin Shen, Liangcai Wu, Wenjing Zhang, Jin Li, Qiuyan
Published in
Lipids in Health and Disease
BackgroundMelanocortin 3 receptor (MC3R), a rhodopsin-like G protein-coupled receptor, is an important regulator of metabolism. Although MC3R knock-out (KO) mice and rats were generated in earlier studies, the function of MC3R remains elusive. Since pig models have many advantages over rodents in metabolism research, we generated an MC3R-KO pig usi...
Morganti, Josh M. Goulding, Danielle S. Van Eldik, Linda J.
Published in
Journal of Neuroinflammation
Traumatic brain injury (TBI) is a significant cause of morbidity and mortality in the USA and other developed countries worldwide. Following the initial mechanical insult, the brain’s primary innate immune effector, microglia, initiate inflammatory signaling cascades and pathophysiological responses that can lead to chronic neuroinflammation and ne...
Greenwald, William W Chiou, Joshua Yan, Jian Qiu, Yunjiang Dai, Ning Wang, Allen Nariai, Naoki Aylward, Anthony Han, Jee Yun Kadakia, Nikita
...
Genetic variants affecting pancreatic islet enhancers are central to T2D risk, but the gene targets of islet enhancer activity are largely unknown. We generate a high-resolution map of islet chromatin loops using Hi-C assays in three islet samples and use loops to annotate target genes of islet enhancers defined using ATAC-seq and published ChIP-se...
Spinazzi, Marco Radaelli, Enrico Horré, Katrien Arranz, Amaia Gounko, Natalia Agostinis, Patrizia Mendes Maia, Teresa Impens, Francis Morais, Vanessa Lopez-Lluch, Guillermo
...
The mitochondrial intramembrane rhomboid protease PARL has been implicated in diverse functions in vitro, but its physiological role in vivo remains unclear. Here we show that ablation in mouse causes a necrotizing encephalomyelopathy similar to Leigh syndrome, a mitochondrial disease characterized by disrupted energy production. Mice with conditio...
Baechler, Simone Factor, Valentina Dalla Rosa, Ilaria Ravji, A Becker, D Khiati, Salim Miller Jenkins, Lisa M Lang, M Sourbier, Carole Michaels, Stephanie
...
Mitochondrial topoisomerase IB (TOP1MT) is a nuclear-encoded topoisomerase, exclusively localized to mitochondria, which resolves topological stress generated during mtDNA replication and transcription. Here, we report that TOP1MT is overexpressed in cancer tissues and demonstrate that TOP1MT deficiency attenuates tumor growth in human and mouse mo...
Kishimoto, Kenta Washio, Youhei Murakami, Yu Katayama, Takashi Kuroyanagi, Miwa Kato, Keitaro Yoshiura, Yasutoshi Kinoshita, Masato
Published in
Fisheries Science
Genome editing technology is becoming increasingly accepted as a way to improve traits in marine fish aquaculture. In fish, microinjection is a major method for introducing RNA or protein into eggs for genome editing; however, this method has not yet been established in aquaculture fish. We successfully established microinjection methods achieving ...
Zare, Khadijeh Shademan, Milad Ghahramani Seno, Mohammad M. Dehghani, Hesam
Published in
Biological Procedures Online
BackgroundWith the increasing discovery of long noncoding RNAs (lncRNAs), the application of functional techniques that could have very specific, efficient, and robust effects and readouts is necessary. Here, we have applied and analyzed three gene knockout (KO) strategies to ablate the CCAT1 gene in different colorectal adenocarcinoma cell lines. ...
Truong, Phan H. Ciccotosto, Giuseppe D. Cappai, Roberto
Published in
Neurochemical Research
The amyloid precursor protein (APP) is a member of a conserved gene family that includes the amyloid precursor-like proteins 1 (APLP1) and 2 (APLP2). APP and APLP2 share a high degree of similarity, and have overlapping patterns of spatial and temporal expression in the central and peripheral tissues, in particular at the neuromuscular junction. AP...
Howells, Rhian M Craze, Melanie Bowden, Sarah Wallington, Emma J
Published in
BMC Plant Biology
BackgroundThe use of CRISPR/Cas9 systems could prove to be a valuable tool in crop research, providing the ability to fully knockout gene function in complex genomes or to precisely adjust gene function by knockout of individual alleles.ResultsWe compare gene editing in hexaploid wheat (Triticum aestivum) with diploid barley (Hordeum vulgare), usin...
Shehata, Hesham M Khan, Shahzada Chen, Elise Fields, Patrick E Flavell, Richard A Sanjabi, Shomyseh
Identifying novel pathways that promote robust function and longevity of cytotoxic T cells has promising potential for immunotherapeutic strategies to combat cancer and chronic infections. We show that sprouty 1 and 2 (Spry1/2) molecules regulate the survival and function of memory CD8+ T cells. Spry1/2 double-knockout (DKO) ovalbumin (OVA)-specifi...
