Ghosh, Anuran Chakrabarti, Rituparna Shukla, Praphulla Chandra
Published in
Journal of Genetic Engineering & Biotechnology
In the last three decades, researchers have utilized genome engineering to alter the DNA sequence in the living cells of a plethora of organisms, ranging from plants, fishes, mice, to even humans. This has been conventionally achieved by using methodologies such as single nucleotide insertion/deletion in coding sequences, exon(s) deletion, mutation...
Kamali, Elahe Rahbarizadeh, Fatemeh Hojati, Zohreh Frödin, Morten
Published in
BMC Biotechnology
BackgroundThe ability of CRISPR/Cas9 to mutate any desired genomic locus is being increasingly explored in the emerging area of cancer immunotherapy. In this respect, current efforts are mostly focused on the use of autologous (i.e. patient-derived) T cells. The autologous approach, however, has drawbacks in terms of manufacturing time, cost, feasi...
Kim, Jung-Hwan Matsubara, Tsutomu Lee, Jaekwon Fenollar-Ferrer, Cristina Han, Kyungreem Kim, Donghwan Jia, Shang Chang, Christopher J Yang, Heejung Nagano, Tomokazu
...
The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes hepatic toxicity associated with prominent lipid accumulation in humans. Here, the authors report that the lysosomal copper transporter SLC46A3 is induced by TCDD and underlies the hepatic lipid accumulation in mice, potentially via effects on mitochondrial function. SL...
Qiao, Fangfang Law, Henry C.-H. Krieger, Kimiko L. Clement, Emalie J. Xiao, Yi Buckley, Shannon M. Woods, Nicholas T.
Published in
Biology Open
Summary: Knockout of Ctdp1 reveals its essential role in mammalian embryogenesis and regulation of the cell cycle.
Morohashi, Ken-Ichirou Inoue, Miki Baba, Takashi
Published in
Endocrinology and metabolism (Seoul, Korea)
The agenesis of the gonads and adrenal gland in revealed by knockout mouse studies strongly suggested a crucial role for Nr5a1 (SF-1 or Ad4BP) in organ development. In relation to these striking phenotypes, NR5A1/Nr5a1 has the potential to reprogram cells to steroidogenic cells, endow pluripotency, and regulate cell proliferation. However, due to l...
Vavers, Edijs Zvejniece, Baiba Stelfa, Gundega Svalbe, Baiba Vilks, Karlis Kupats, Einars Mezapuke, Rudolfs Lauberte, Lasma Dambrova, Maija Zvejniece, Liga
...
Published in
Neurobiology of disease
There is a growing body of evidence demonstrating the significant involvement of the sigma-1 chaperone protein in the modulation of seizures. Several sigma-1 receptor (Sig1R) ligands have been demonstrated to regulate the seizure threshold in acute and chronic seizure models. However, the mechanism by which Sig1R modulates the excitatory and inhibi...
Zhang, Xue Yang, Yuxi Bu, Xiaoxue Wei, Yuanyuan Lou, Xin
Published in
Heliyon
Major vault protein, Regeneration, Zebrafish, Knockout, Anti-apoptotic effect, Biological sciences, Cell biology, Cell death, Developmental genetics, Regenerative medicine.
Lin, Ziguang Jin, Suoqin Chen, Jefferson Li, Zhuorui Lin, Zhongqi Tang, Li Nie, Qing Andersen, Bogi
The interfollicular epidermis (IFE) forms a water-tight barrier that is often disrupted in inflammatory skin diseases. During homeostasis, the IFE is replenished by stem cells in the basal layer that differentiate as they migrate toward the skin surface. Conventionally, IFE differentiation is thought to be stepwise as reflected in sharp boundaries ...
Hultgren, Nan W Fang, Jennifer S Ziegler, Mary E Ramirez, Ricardo N Phan, Duc TT Hatch, Michaela MS Welch-Reardon, Katrina M Paniagua, Antonio E Kim, Lin S Shon, Nathan N
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Slug (SNAI2), a member of the well-conserved Snail family of transcription factors, has multiple developmental roles, including in epithelial-to-mesenchymal transition (EMT). Here, we show that Slug is critical for the pathological angiogenesis needed to sustain tumor growth, and transiently necessary for normal developmental angiogenesis. We find ...
McQuade, Amanda Kang, You Jung Hasselmann, Jonathan Jairaman, Amit Sotelo, Alexandra Coburn, Morgan Shabestari, Sepideh Kiani Chadarevian, Jean Paul Fote, Gianna Tu, Christina H
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The discovery of TREM2 as a myeloid-specific Alzheimer's disease (AD) risk gene has accelerated research into the role of microglia in AD. While TREM2 mouse models have provided critical insight, the normal and disease-associated functions of TREM2 in human microglia remain unclear. To examine this question, we profile microglia differentiated from...
