Kütt, Jaak Margus, Georg Kask, Lauri Rätsepso, Triinu Soodla, Kärol Bernasconi, Romain Birkedal, Rikke Järv, Priit Laasmaa, Martin Vendelin, Marko
...
Published in
BMC biology
Current solutions for the analysis of Western Blot images lack either transparency and reproducibility or can be tedious to use if one has to ensure the reproducibility of the analysis. Here, we present an open-source gel image analysis program, IOCBIO Gel. It is designed to simplify image analysis and link the analysis results with the metadata de...
Danchana, Kaewta Yamashita, Nayu Umeda, Mika I. Kaneta, Takashi
We demonstrated the fractionation of two amino acids, glutamic acid and histidine, separated via isoelectric focusing (IEF) on filter paper folded and stacked in an origami fashion. Channels for electrophoresis were fabricated as circular zones acquired via wax printing onto the filter paper. An ampholyte solution with amphiphilic samples was depos...
Kamata, Shotaro Ishii, Isao
Published in
Methods in molecular biology (Clifton, N.J.)
Several years have passed since LC (liquid chromatography)-MS (mass spectrometry) became the mainstream for proteomic analysis; however, conventional fluorescence two-dimensional difference gel electrophoresis (2D-DIGE) continues to be an important technology that enables rapid and direct visualization of hundreds to thousands of proteins and their...
Di Luca, Alessio Hamill, Ruth Mullen, Anne Maria Elia, Giuliano
Published in
Methods in molecular biology (Clifton, N.J.)
Two-dimensional difference gel electrophoresis (2D-DIGE) is an acrylamide gel electrophoresis-based technique for protein separation and quantification in complex mixtures. The technique addresses some of the drawbacks of conventional 2D polyacrylamide gel electrophoresis (2D-PAGE), offering improved sensitivity, more limited experimental variation...
Holland, Ashling
Published in
Methods in molecular biology (Clifton, N.J.)
The global analysis of the proteome is an important tool in cell biology. Comparative proteomic evaluations can identify and compare the composition, dynamics, and modifications between different samples. Comparing tissue proteomes under different conditions is crucial for advancing the biomedical field. Fluorescence two-dimensional difference gel ...
Thormann, Wolfgang Mosher, Richard A
Published in
Electrophoresis
The transport of components in liquid media under the influence of an applied electric field can be described with the continuity equation. It represents a nonlinear conservation law that is based upon the balance laws of continuous transport processes and can be solved in time and space numerically. This procedure is referred to as dynamic compute...
Cabrera, Carmen M
Published in
Advances in clinical chemistry
Oligoclonal bands (OCBs) in cerebrospinal fluid (CSF) represent an indicator of IgG and IgM immunoglobulins intrathecal synthesis in the central nervous system (CNS). The techniques and detection methods for their determination have evolved from the beginning to isoelectric focusing on an agarose gel as the gold standard technique and immunodetecti...
Troung, Stanley F Sukhodolets, Maxim V
Published in
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
In bacteria transcription is coupled to translation, and while it is broadly accepted that transcription-translation complexes (TTCs) are formed in growing bacterial cells, the exact spatial organization of these macromolecular assemblies is not known with certainty. Recent studies indicated the formation of orderly cytosolic superstructures in gro...
Farmerie, Lily Rustandi, Richard R Loughney, John W Dawod, Mohamed
Published in
Journal of chromatography. A
This review article describes the significant recent advances in Isoelectric Focusing from the period 2015-2020. The review highlights the principles and common challenges faced in Isoelectric Focusing as well as its applications. This review also details the recent advances in various modes of Isoelectric Focusing in various platforms and future d...
Thormann, Wolfgang Mosher, Richard A
Published in
Electrophoresis
Electrokinetic processes that lead to pH gradient instabilities in carrier ampholyte-based IEF are reviewed. In addition to electroosmosis, there are four of electrophoretic nature, namely (i) the stabilizing phase with the plateau phenomenon, (ii) the gradual isotachophoretic loss of carrier ampholytes at the two column ends in presence of electro...
